Electrocatalysis

, Volume 4, Issue 1, pp 17–23 | Cite as

Electrochemical Detection of Low-Molecular-Mass Biothiols in Biological Fluids at Carbon Spheres-Modified Glassy Carbon Electrodes

  • Guozhi Wu
  • Jianqing Zhou
  • Xueyue Jiang
  • Xinying Guo
  • Feng Gao
Article
First Online:

Abstract

The excellent electrocatalytic activity of a new kind of microstructured carbon material, carbon hollow spheres (CS), toward the oxidation of low-molecular-mass biological thiols including cysteine, homocysteine, and glutathione has been investigated. Comparing with conventional bare glassy carbon (GC) electrodes, an overpotential of 0.40 V is reduced at CS-modified GC electrode with electrooxidation starting at 0.0 V (vs. Ag/AgCl). Based on the electrocatalytic activity of CS, a direct electrochemical method for the determination of low-molecular-mass biothiols is proposed. The proposed method was applied to the determination of biothiols in human plasma and urine with satisfactory results.

Keywords

Electrocatalytic oxidation Low-molecular-mass biothiols Carbon hollow spheres Biological fluids 
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Notes

Acknowledgments

F. Gao is grateful for the financial support from the Natural Science Foundation of China (grant nos. 21175002) and the Anhui Provincial Natural Science Foundation for Distinguished Youth (no. 1108085J09) for the financial support.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Guozhi Wu
    • 1
    • 2
  • Jianqing Zhou
    • 3
  • Xueyue Jiang
    • 4
  • Xinying Guo
    • 1
  • Feng Gao
    • 1
  1. 1.Key Laboratory of Chemo/Biosensing, College of Chemistry and Materials ScienceAnhui Normal UniversityWuhuChina
  2. 2.Department of Chemistry and Food ScienceChizhou CollegeChizhouChina
  3. 3.Anhui Medical CollegeHefeiChina
  4. 4.College of Chemistry and Chemical EngineeringFuyang Teachers CollegeFuyangChina

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