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A Novel Branched Copolymer-Containing Anticancer Drug for Targeted Therapy: In Vitro Research

  • A. Yurchenko
  • N. Nikitina
  • V. Sokolova
  • S. Prylutska
  • Yu. KuzivEmail author
  • P. Virych
  • V. Chumachenko
  • N. Kutsevol
  • S. Ponomarenko
  • Yu. Prylutskyy
  • M. Epple
Article

Abstract

The viability of cancer cell lines (human transformed cervix epithelial cells, HeLa, and osteoblastic cell line from a C57BL/6 mouse calvaria, MC3T3, as models for cancer cells) was studied using MTT and live/dead assays after incubation with a branched copolymer dextran-graft polyacrylamide in anionic form (D-g-PAAan) as nanocarrier for drugs, doxorubicin (Dox), cisplatin (Cis), as well as their D-g-PAAan+Dox and D-g-PAAan+Cis mixtures, as a function of the concentration. Fourier transform infrared spectroscopy clearly indicates the complex formation of Cis and Dox with the D-g-PAAan branched copolymer. The size distribution of particles in aqueous solution and its stability were determined by dynamic light scattering. The in vitro uptake of studied particles into cancer cells was demonstrated by confocal laser scanning microscopy. It was found that D-g-PAAan+Dox particles in contrast to Dox alone showed higher toxicity towards cancer cells. This indicates the possibility of further preclinical studies of the water-soluble D-g-PAAan+Dox particles on animal tumor models in vivo as a promising anticancer agent.

Keywords

Doxorubicin Cisplatin Branched copolymer HeLa and MC3T3 cells Cytotoxicity 

Notes

Acknowledgments

We thank the Imaging Centre Campus Essen (ICCE) for access to the CLSM.

Funding Information

A. Yurchenko and N. Nikitina are grateful to Deutscher Akademischer Austauschdienst (DAAD) for financial support within the Leonhard-Euler program. This work was also partly supported by the Bilaterial Ukrainian-Belarusian Scientific Project.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Research Involving Humans and Animals Statement

None

Informed Consent

None

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.ESC “Institute of Biology and MedicineTaras Shevchenko National University of KyivKyivUkraine
  2. 2.Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE)University of Duisburg-EssenEssenGermany
  3. 3.Faculty of ChemistryTaras Shevchenko National University of KyivEssenUkraine

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