Biosynthesis, Characterization and Structural Properties of a Novel Kind of Ag/ZnO Nanocomposites In Order to Increase Its Biocompatibility Across Human A549 Cell Line

  • Saeed JafariradEmail author
  • Parya M. Taghizadeh
  • Baharak Divband


Recently, Ag/ZnO nanocomposite (Ag/ZnONC) has been known as one of effective antimicrobial agents. However, its interesting aspects could cause several health effects. This problem can be rectified by capping its surface via the addition of a biomolecule which form a “bio-capped layer” around the particles and prevent them from interacting human cells. In this study, a new fabrication of bio-capped Ag/ZnONCs in various amounts of silver has been reported. Stable and spherical bio-capped Ag/ZnONCs were produced using extract which acts as a reducing and capping agent. The physicochemical, properties of the fabricated NCs was characterized by UV-Vis, XRD, DLS, zeta potential, SEM, XRD, EDX, and FTIR, antioxidant activity. In addition, the mechanism of fabrication has been suggested. Our method of fabrication could be an eco-friendly technique and considered as an alternative to other techniques such as electro- and photo-chemical, chemical, and physical techniques. Our results show that the as-synthesized Ag/ZnONCs possess antioxidant activity and the secondary metabolites of the extract adsorbed on the NC reacts with DPPH. Moreover, the cytotoxicity of bio-capped Ag/ZnONCs (0.15% of Ag) to treat cell lines was evaluated by using cell viability test of human A549 cell line. The obtained results demonstrated that concentrations of up to 0.1 mg/mL of bio-capped Ag/ZnONCs induced no significant harmful to the cells. Hence, the present study proved that the fabricated NCs have the potential to use in industrial and pharmacological applications.


Cytotoxicity analysis Human A549 cell line Ag/ZnO nanocomposites Biocompatibility 


Funding Information

This project is financially supported by the University of Tabriz (grant no. 3381).

Compliance with ethical standards

Conflict of interest


Research involving humans and animals statement


Informed consent


Funding statement



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Authors and Affiliations

  1. 1.Research Institute for Fundamental Sciences (RIFS)University of TabrizTabrizIran
  2. 2.Research Center for Pharmaceutical NanotechnologyTabriz University of Medical SciencesTabrizIran
  3. 3.Inorganic Chemistry Department, Faculty of ChemistryUniversity of TabrizTabrizIran

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