, Volume 9, Issue 4, pp 773–777 | Cite as

Effect of Some Charged Polymers on the Activity of Pancreatic Porcine Lipase

  • Sergei Yu. ZaitsevEmail author
  • Anastasia A. Savina
  • Lilia S. Garnashevich
  • Marina S. Tsarkova
  • Ilia S. Zaitsev


Lipases play an important role in numerous metabolic reactions in all living species and may be activated at interfaces by some biopolymers in vivo, but inhibited—in vitro. The effect of two chitosan samples with different molecular weights on the activity of lipase from porcine pancreas (LPP) in triacetin hydrolysis was investigated. An equimolar amount or excess of chitosan activates LPP by a factor of 1.2 to 1.6. In order to support these data, a negatively charged polyelectrolyte—sodium polystyrene sulfonate (PSS)—was used instead of chitosan. A small increase in LPP activity was found also at an equimolar amount or excess of PSS. This unexpected effect can be explained as “interfacial activation” of LPP with opening of the hydrophobic channel for triacylglycerol hydrolysis. The observed activation effect is important for fundamental biochemistry of human and animal digestion models, as well as for further lipase applications.

Graphical abstract

Possible molecular interactions between chitosan and lipase from porcine pancreas


Lipase Chitosan Enzyme immobilization Activity Triacetin 


Funding Information

This work was financially supported by the Russian Foundation for Basic Research (RFBR project 19-03-00717).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Chemistry DepartmentFederal State Budgetary Educational Institution of Higher Education “Moscow State Academy of Veterinary Medicine and Biotechnology - MVA after K.I. Skryabin”MoscowRussia
  2. 2.Institute of Bioorganic Chemistry RASMoscowRussia

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