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BioNanoScience

, Volume 9, Issue 1, pp 44–47 | Cite as

Heterologous Expression of Histidine Acid Phytase from Pantoea sp. 3.5.1 in Yarrowia lipolytica

  • Aliya D. SuleimanovaEmail author
  • Daria S. Troshagina
  • Catherine Madzak
  • Margarita R. Sharipova
Article
  • 16 Downloads

Abstract

The dimorphic yeasts Yarrowia lipolytica are used as effective expression system and are characterized by a high level of production of heterologous protein. In this work, we aimed to clone and express Pantoea sp. 3.5.1 agpP phytase gene in Yarrowia lipolytica. Genetic constructs containing the native phytase gene (agpP) under the control of the strong hybrid promoter hp4d and the signal peptide of the alkaline extracellular protease XPR2 gene, as well as the optimized gene (agpP-opt) of phytase under the control of two signal peptides—bacterial and yeast were obtained. Recombinant Y. lipolytica strains with integrated bacterial phytase genes were obtained and expression of phytase was analyzed.

Keywords

Phytase Cloning Heterologous expression Yarrowia 

Notes

Acknowledgments

This work was performed in accordance with the Russian Government Program of Competitive Growth of the Kazan Federal University and supported by the Russian Foundation for Basic Research (project no. 16-34-60191).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Kazan (Volga region) Federal UniversityKazanRussia
  2. 2.Université Paris-SaclayThiverval-GrignonFrance

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