, Volume 8, Issue 3, pp 930–934 | Cite as

Antibiotic Susceptibility Assessment of Helicobacter pylori Isolates by Disk-Diffusion Method

  • A. R. Akhtereeva
  • L. G. Morozova
  • R. A. Faizullina
  • K. A. Ivanovskaya
  • O. K. Pozdeev
  • I. Kh. Valeeva
  • S. R. AbdulkhakovEmail author


Helicobacter pylori (H. pylori) infection is tightly associated with gastrointestinal disorders such as chronic gastritis, peptic ulcer, gastric MALToma, and gastric cancer. Decreased antibiotic susceptibility in H. pylori is a worldwide problem. Our objective was to determine in vitro antimicrobial susceptibility of H. pylori isolates obtained from gastric mucosa biopsies of children with H. pylori-associated gastroduodenal diseases using disk-diffusion method. A total 76 biopsy specimens were studied; antibiotic susceptibility was assessed in case of 30 children in whom H. pylori was revealed by bacteriology. The maximum resistance of H. pylori isolates was revealed to clarithromycin with nine resistant isolates (30.0%). The rate of resistance to metronidazole, amoxicillin, furazolidone, tetracycline, and levofloxacin was 23.3, 33.3, 16.7, 25.0, and 16.7%, respectively. Multidrug resistance was detected in 20.0% of H. pylori strains. The high prevalence of resistance to antibiotics used in eradication therapy is becoming a problem which needs eradication therapy regimen use based on regional H. pylori resistance rates.


Helicobacter pylori Children Antibiotic susceptibility Disk-diffusion method 


Funding Information

This study was supported by the Russian Government Program of Competitive Growth of Kazan Federal University.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Kazan State Medical UniversityKazanRussia
  2. 2.Institute of Fundamental Medicine and BiologyKazan (Volga Region) Federal UniversityKazanRussia
  3. 3.Kazan State Medical AcademyKazanRussia
  4. 4.Republican Clinical Infectious Diseases Hospital named after A.F. AgafonovKazanRussia

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