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BioNanoScience

, Volume 8, Issue 3, pp 868–883 | Cite as

A Review on 3D Printable Techniques for Tissue Engineering

  • Sharda Gupta
  • Akalabya Bissoyi
  • Arindam BitEmail author
Article

Abstract

A rapid revolution in the medical health care sector is expected due to the use of medical products fabricated from 3D printer technology. Several researches have been carried on the innovative achievements of biomaterials using 3D printing. This advancement in biomaterials leads to an increasing trend in the use of 3D printer even in medical research. During 3D printing, the materials are transformed into desired shape with the application of heat, laser, or other energies. Hence, as per the mechanical property, materials react towards the external energy to define the design capability. 3D printing technology has the ability to convert the two-dimensional image obtained from non-invasive scanning imaging of human body, i.e., computed tomography (CT) or magnetic resonance imaging (MRI) scan into a 3D digital model of any shape. The combined uses of biomaterials and 3D printing technologies have made significant improvement in the field of tissue engineering. Due to shortage of donor organs and also lack of facility to preserve them leads to the demand of artificial tissues. In this review paper, we firstly introduce about the various types of conventional and recent 3D printing technologies, biomaterials, and additives which changes its physical properties. And finally, summarize the applications of 3D printing technique in medical applications. There is the possibility to increase resolution and print single cell layer using piezoelectric transducer at the printer head in FDM-based printing technique which is described as the proposed technique in the paper.

Keywords

3D printing Bio-materials Microfluidics Tissue engineering 3D cell printing 

Notes

Acknowledgements

Authors are grateful to the National Institute of Technology, Raipur (CG), India, for providing the necessary facilities for this work.

Funding Information

This study was supported by a grant from the Department of Science and Technology (YSS/2015/000618 and ECR/2017/001115) New Delhi, India.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Williams, C. B. (2015). The status, challenges, and future of additive manufacturing in engineering. Computer Design, 69, 65–89.Google Scholar
  2. 2.
    Christ, S., Schnabel, M., Vorndran, E., Groll, J., & Gbureck, U. (2015). Fiber reinforcement during 3D printing. Materials Letters, 139, 165–168.Google Scholar
  3. 3.
    Schubert, C., Van Langeveld, M. C., and Donoso, L. A. (2013). Innovations in 3D printing: a 3D overview from optics to organs. pp. 3–6.Google Scholar
  4. 4.
    Gholipourmalekabadi, M., Zhao, S., Harrison, B. S., Mozafari, M., & Seifalian, A. M. (2016). Oxygen-generating biomaterials: a new, viable paradigm for tissue engineering? Trends in Biotechnology, 34(12), 1010–1021.Google Scholar
  5. 5.
    Yung, S. and Factors, I. T. (2002). The design of scaffolds for use in tissue engineering. Part I. Traditional factors.Google Scholar
  6. 6.
    Chen, F. and Liu, X. (2015). Ac ce pt e cr ip t. Progress in Polymer Science.Google Scholar
  7. 7.
    Murdock, M. H. and Badylak, S. F. (2017). Biomaterials-based in situ tissue engineering. Current Opinion in Biomedical Engineering.Google Scholar
  8. 8.
    Santoro, M., Shah, S. R., Walker, J. L., and Mikos, A. G. (2016). Poly (lactic acid) nano fi brous scaffolds for tissue engineering. Advanced Drug Delivery Reviews.Google Scholar
  9. 9.
    Haddad, T., Noel, S., and De Crescenzo, G. (2016). Fabrication and surface modification of poly lactic acid ( PLA ) scaffolds with epidermal growth factor for neural tissue engineering. 6(1):1–12.Google Scholar
  10. 10.
    Karp, J. M., Shoichet, M. S., and Davies, J. E. (2002). Bone formation on two-dimensional poly ( DL-lactide- co-glycolide ) (PLGA) films and three-dimensional PLGA tissue engineering scaffolds in vitro.Google Scholar
  11. 11.
    Holy, C. E., Cheng, C., Davies, J. E., and Shoichet, M. S. (2001). Optimizing the sterilization of PLGA scaffolds for use in tissue engineering. 22:25–31.Google Scholar
  12. 12.
    Sahoo, S., Toh, S. L., and Goh, J. C. H. (2017). A bFGF-releasing Silk/PLGA-based biohybrid scaffold for ligament/tendon tissue engineering using biomaterials ... 31(11):2990–2998.Google Scholar
  13. 13.
    “Scaffold development using selective laser sintering of polyetheretherketone–hydroxyapatite biocomposite blends,” no. September 2003, 2017.Google Scholar
  14. 14.
    Rosenzweig, D., Carelli, E., Steffen, T., Jarzem, P., & Haglund, L. (2015). 3D-printed ABS and PLA scaffolds for cartilage and nucleus pulposus tissue regeneration. International Journal of Molecular Sciences, 16(7), 15118–15135.Google Scholar
  15. 15.
    Rau, J. V., Antoniac, I., Cama, G., Komlev, V. S., and Ravaglioli, A. (2016). Bioactive materials for bone tissue engineering. vol. 2016.Google Scholar
  16. 16.
    Di, A., Sittinger, M., and Risbud, M. V. (2005). Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. 26:5983–5990.Google Scholar
  17. 17.
    Engineering, T., Akzonobel, R. K., and Francisco, S. (2001). Evaluation of nanostructured composite collagen—chitosan matrices for tissue evaluation of nanostructured composite.Google Scholar
  18. 18.
    Williams, J. M. et al. (2005). Bone tissue engineering using polycaprolactone scaffolds fabricated via selective laser sintering. 26:4817–4827.Google Scholar
  19. 19.
    You, W., Siu, Z. Æ., Lee, H., and Wang, Æ. M. (2008). Selective laser sintering of porous tissue engineering scaffolds from poly ( L-lactide)/carbonated hydroxyapatite nanocomposite microspheres.pp. 2535–2540.Google Scholar
  20. 20.
    Ingrowth, B., Cooke, M. N., Fisher, J. P., Dean, D., Rimnac, C., and Mikos, A. G. (2015). Use of stereolithography to manufacture critical-sized 3D biodegradable scaffolds for bone ingrowth.Google Scholar
  21. 21.
    Q. U. T. D. Repository (2009). This is the accepted version of this journal article: Melchels , Ferry P . W . and Feijen , Jan and Grijpma , Dirk A poly ( D , L-lactide ) resin for the preparation of tissue engineering scaffolds by stereolithography. 30.Google Scholar
  22. 22.
    R. Liska, “Processing of 45S5 Bioglass (R) by lithography-based additive manufacturing,” no. April 2014, 2012.Google Scholar
  23. 23.
    W. Zhu, X. Ma, M. Gou, D. Mei, K. Zhang, and S. Chen, “Science direct 3D printing of functional biomaterials for tissue engineering,” Current Opinion in Biotechnology, vol. 40, pp. 103–112, 2016.Google Scholar
  24. 24.
    Hutmacher, D. W. (2000). Scaffolds in tissue engineering bone and cartilage. 21:2529–2543.Google Scholar
  25. 25.
    Sittinger, M., Hutmacher, D. W., Sittinger, M., and Risbud, M. V. (2004). Scaffold-based tissue engineering: rationale for computer-aided design and solid free-form fabrication systems.Google Scholar
  26. 26.
    Yeong, W., Chua, C., Leong, K., and Chandrasekaran, M. (2005). Trends in rapid prototyping in tissue engineering: challenges and potential. 22:643–652.Google Scholar
  27. 27.
    Tan, K. (2002). Fused deposition modeling of novel scaffold architectures for tissue engineering applications.Google Scholar
  28. 28.
    Cukierman, E., Pankov, R., Stevens, D. R., and Yamada, K. M. (2001). Taking cell-matrix adhesions to the third dimension. 294:1708–1712.Google Scholar
  29. 29.
    Duan, B. and Lu, W. W. (2010). Three-dimensional nanocomposite scaffolds fabricated via selective laser sintering for bone tissue engineering.Google Scholar
  30. 30.
    Yi, H., Lee, H., and Cho, D. (2017). 3D printing of organs-on-chips.Google Scholar
  31. 31.
    Heidari Kani, M., Chan, E. C., Young, R. C., Butler, T., Smith, R., and Paul, J. W. (2016). 3D cell culturing and possibilities for myometrial tissue engineering. Annals of Biomedical Engineering, 1–12.Google Scholar
  32. 32.
    Vadivelu, R. K., Kamble, H., Shiddiky, M. J. A., and Nguyen, N. (2017). Microfluidic technology for the generation of cell spheroids and their applications. pp. 1–23.Google Scholar
  33. 33.
    Li et al. (2014). NIH public access. 4(12):1509–1525.Google Scholar
  34. 34.
    Albrecht, L. D., Sawyer, S. W., & Soman, P. (2016). Developing 3D scaffolds in the field of tissue engineering to treat complex bone defects. 3D Printing and Additive Manufacturing, 3(2), 106–112.Google Scholar
  35. 35.
    Lu, T., Li, Y., & Chen, T. (2013). Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering. International Journal of Nanomedicine, 8, 337–350.Google Scholar
  36. 36.
    A. Cells, M. Sciences, M. Sciences, M. Sciences, and M. Sciences (2016). Biodegradable and biocompatible polymers for tissue engineering application: a reviewGoogle Scholar
  37. 37.
    Bajaj, P., Schweller, R. M., Khademhosseini, A., West, J. L., and Bashir, R. (2014). 3D biofabrication strategies for tissue engineering and regenerative medicine. Maydica.Google Scholar
  38. 38.
    Scheithauer, U., Schwarzer, E., and Moritz, T. (2015). Thermoplastic 3D printing—an additive manufacturing method for producing dense ceramics. 31:26–31.Google Scholar
  39. 39.
    Qiu, J., & Wei, J. (2015). Additive manufacturing of carbon fiber reinforced thermoplastic composites using fused deposition modeling. Composites Part B, 80, A7–A15.Google Scholar
  40. 40.
    Bikas, H., Stavropoulos, P., and Chryssolouris, G. (2016). Additive manufacturing methods and modelling approaches: a critical review. pp. 389–405.Google Scholar
  41. 41.
    Ji, S. and Guvendiren, M. (2017). Recent advances in bioink design for 3D bioprinting of tissues and organs. 5:1–8.Google Scholar
  42. 42.
    Domingos, M., et al. (2013). The first systematic analysis of 3D rapid prototyped poly(ε-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and in vitro hMSC viability. Biofabrication, 5(4), 45004.Google Scholar
  43. 43.
    Boere, K. W. M., et al. (2015). Biofabrication of reinforced 3D-scaffolds using two-component hydrogels. Journal of Materials Chemistry B, 3(46), 9067–9078.Google Scholar
  44. 44.
    Muerza-Cascante, M. L., Haylock, D., Hutmacher, D. W., & Dalton, P. D. (2015). Melt electrospinning and its technologization in tissue engineering. Tissue Engineering. Part B, Reviews, 21(2), 187–202.Google Scholar
  45. 45.
    Wang, H., Vijayavenkataraman, S., Wu, Y., Shu, Z., and Sun, J. (2016). Investigation of process parameters of electrohydro-dynamic jetting for 3D printed PCL fibrous scaffolds with complex geometries. p. 1–9.Google Scholar
  46. 46.
    Wei, C., & Dong, J. (2014). Hybrid hierarchical fabrication of three-dimensional scaffolds. Journal of Manufacturing Processes, 16(2), 257–263.Google Scholar
  47. 47.
    Cao, T., Ho, K.-H., & Teoh, S.-H. (2003). Scaffold design and in vitro study of osteochondral coculture in a three-dimensional porous polycaprolactone scaffold fabricated by fused deposition modeling. Tissue Engineering, 9(Suppl 1), S103–S112.Google Scholar
  48. 48.
    Rezayat, H., Zhou, W., Siriruk, A., Penumadu, D., & Babu, S. S. (2015). Structure–mechanical property relationship in fused deposition modelling. Materials Science and Technology, 31(8), 895–903.Google Scholar
  49. 49.
    Malinauskas, M., et al. (2014). 3D microporous scaffolds manufactured via combination of fused filament fabrication and direct laser writing ablation. Micromachines, 5(4), 839–858.Google Scholar
  50. 50.
    Tuan Rahim, T. N. A., Abdullah, A. M., Md Akil, H., Mohamad, D., & Rajion, Z. A. (2015). Preparation and characterization of a newly developed polyamide composite utilising an affordable 3D printer. Journal of Reinforced Plastics and Composites, 34(19), 1628–1638.Google Scholar
  51. 51.
    Qin, Z., Compton, B. G., Lewis, J. A., & Buehler, M. J. (2015). Structural optimization of 3D-printed synthetic spider webs for high strength. Nature Communications, 6, 8038.Google Scholar
  52. 52.
    E. L. Melgoza, G. Vallicrosa, L. Serenó, J. Ciurana, and C. A. Rodríguez, Rapid tooling using 3D printing system for manufacturing of customized tracheal stent,” Rapid Prototyping Journal, vol. 20, no. 1, pp. 2–12, 2013.Google Scholar
  53. 53.
    Vaezi, M., & Yang, S. (2015). A novel bioactive PEEK/HA composite with controlled 3D interconnected HA network. Journal of Bioprinting, 1(1), 66–76.Google Scholar
  54. 54.
    Vaezi, M., & Yang, S. (2015). Extrusion-based additive manufacturing of PEEK for biomedical applications. Virtual and Physical Prototyping, 10(3), 123–135.Google Scholar
  55. 55.
    Wu, W. Z., Geng, P., Zhao, J., Zhang, Y., Rosen, D. W., & Zhang, H. B. (2014). Manufacture and thermal deformation analysis of semicrystalline polymer polyether ether ketone by 3D printing. Materials Research Innovations, 18(sup5), S5–12–S5–16.Google Scholar
  56. 56.
    Wu, W., Geng, P., Li, G., Zhao, D., Zhang, H., and Zhao, J. (2015). Influence of layer thickness and raster angle on the mechanical properties of 3D-printed PEEK and a comparative mechanical study between PEEK and ABS. pp. 5834–5846.Google Scholar
  57. 57.
    Zhao, Y., et al. (2014). Three-dimensional printing of Hela cells for cervical tumor model in vitro. Biofabrication, 6(3), 35001.Google Scholar
  58. 58.
    Zhang, X., Zhu, Z., Ni, Z., Xiang, N., & Yi, H. (2017). Inexpensive, rapid fabrication of polymer-film microfluidic autoregulatory valve for disposable microfluidics. Biomedical Microdevices, 19(2), 21.Google Scholar
  59. 59.
    Jia, J., et al. (2014). Engineering alginate as bioink for bioprinting. Acta Biomaterialia, 10(10), 4323–4331.Google Scholar
  60. 60.
    Ouyang, L., Yao, R., Chen, X., Na, J., & Sun, W. (2015). 3D printing of HEK 293FT cell-laden hydrogel into macroporous constructs with high cell viability and normal biological functions. Biofabrication, 7(1), 15010.Google Scholar
  61. 61.
    Ozbolat, I. T., Chen, H., & Yu, Y. (2014). Robotics and computer-integrated manufacturing development of ‘multi-arm bioprinter’ for hybrid biofabrication of tissue engineering constructs. Robotics and Computer-Integrated Manufacturing, 30(3), 295–304.Google Scholar
  62. 62.
    Zhang, Y., Yu, Y., Chen, H., & Ozbolat, I. T. (2013). Characterization of printable cellular micro-fluidic channels for tissue engineering. Biofabrication, 5(2), 25004.Google Scholar
  63. 63.
    Christensen, K., Xu, C., Chai, W., Zhang, Z., Fu, J., & Huang, Y. (2015). Freeform inkjet printing of cellular structures with bifurcations. Biotechnology and Bioengineering, 112(5), 1047–1055.Google Scholar
  64. 64.
    Liu, C. Z., Xia, Z. D., Han, Z. W., Hulley, P. A., Triffitt, J. T., & Czernuszka, J. T. (2008). Novel 3D collagen scaffolds fabricated by indirect printing technique for tissue engineering. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 85(2), 519–528.Google Scholar
  65. 65.
    Duarte Campos, D. F., et al. (2015). The stiffness and structure of three-dimensional printed hydrogels direct the differentiation of mesenchymal stromal cells toward adipogenic and osteogenic lineages. Tissue Engineering. Part A, 21(3–4), 740–756.Google Scholar
  66. 66.
    Ng, W. L., Yeong, W. Y., & Naing, M. W. (2016). Polyelectrolyte gelatin-chitosan hydrogel optimized for 3D bioprinting in skin tissue engineering. International Journal of Bioprinting, 2(1), 53–62.Google Scholar
  67. 67.
    Gong, H., Agustin, J., Wootton, D., & Zhou, J. G. (2014). Biomimetic design and fabrication of porous chitosan-gelatin liver scaffolds with hierarchical channel network. Journal of Materials Science. Materials in Medicine, 25(1), 113–120.Google Scholar
  68. 68.
    Shao, Y., Chaussy, D., Grosseau, P., & Beneventi, D. (2015). Use of microfibrillated cellulose/lignosulfonate blends as carbon precursors: impact of hydrogel rheology on 3D printing. Industrial and Engineering Chemistry Research, 54(43), 10575–10582.Google Scholar
  69. 69.
    Seitz, H., Rieder, W., Irsen, S., Leukers, B., & Tille, C. (2005). Three-dimensional printing of porous ceramic scaffolds for bone tissue engineering. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 74(2), 782–788.Google Scholar
  70. 70.
    Shao, H., et al. (2015). 3D printing magnesium-doped wollastonite/β-TCP bioceramics scaffolds with high strength and adjustable degradation. Journal of the European Ceramic Society, 1–9.Google Scholar
  71. 71.
    Wang, X., et al. (2017). A 3D-printed scaffold with MoS2 nanosheets for tumor therapy and tissue regeneration. NPG Asia Materials, 9(4), e376.Google Scholar
  72. 72.
    Schacht, K., Jüngst, T., Schweinlin, M., Ewald, A., Groll, J., & Scheibel, T. (2015). Biofabrication of cell-loaded 3D spider silk constructs. Angewandte Chemie, International Edition, 54(9), 2816–2820.Google Scholar
  73. 73.
    Melchels, F. P. W., Dhert, W. J. a., Hutmacher, D. W., & Malda, J. (2014). Development and characterisation of a new bioink for additive tissue manufacturing. Journal of Materials Chemistry B, 2, 2282.Google Scholar
  74. 74.
    Bertassoni, L. E., et al. (2014). Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels. Biofabrication, 6(2), 24105.Google Scholar
  75. 75.
    Adamkiewicz, M., & Rubinsky, B. (2015). Cryogenic 3D printing for tissue engineering. Cryobiology, 71(3), 518–521.Google Scholar
  76. 76.
    Glatzel, S., Hezwani, M., Kitson, P. J., Gromski, P. S., Schürer, S., & Cronin, L. (2016). A portable 3D printer system for the diagnosis and treatment of multidrug-resistant bacteria. Chem, 1(3), 494–504.Google Scholar
  77. 77.
    Habib, F. N., Nikzad, M., Masood, S. H., & Saifullah, A. B. M. (2016). Design and development of scaffolds for tissue engineering using three-dimensional printing for bio-based applications. 3D Printing and Additive Manufacturing, 3(2), 119–127.Google Scholar
  78. 78.
    Urrios, A., et al. (2016). 3D-printing of transparent bio-microfluidic devices in PEG-DA. Lab on a Chip, 16(12), 2287–2294.Google Scholar
  79. 79.
    Tasoglu, S., & Demirci, U. (2013). Bioprinting for stem cell research. Trends in Biotechnology, 31(1), 10–19.Google Scholar
  80. 80.
    “Biosurface engineering through ink jet printing,” no. September, 2009.Google Scholar
  81. 81.
    Nakamura, M. et al. (2005). Biocompatible Inkjet printing technique for designed seeding of individual living cells. 11(11):1658–1666.Google Scholar
  82. 82.
    Lotz, M., Cui, X., Boland, T., Lima, D. D. D., and Lotz, M. K. (2012). Thermal inkjet printing in tissue engineering and regenerative medicine Thermal Inkjet Printing in Tissue Engineering and Regenerative Medicine.Google Scholar
  83. 83.
    R. Article (2013). Chemical Society Reviews.Google Scholar
  84. 84.
    Murphy, S. V., & Atala, A. (2014). Review 3D bioprinting of tissues and organs. Nature Publishing Group, 32(8), 773–785.Google Scholar
  85. 85.
    Ning, L. and Chen, X. (2017). A brief review of extrusion-based tissue scaffold bio-printing. pp. 1–16.Google Scholar
  86. 86.
    Pati, F., Jang, J., Lee, J. W., and Cho, D. (2015). Extrusion bioprinting.Google Scholar
  87. 87.
    Kutikov, A. B., Gurijala, A., and Song, J. (2014). Rapid prototyping amphiphilic polymer/hydroxyapatite composite scaffolds with hydration-induced self-fixation behavior. Tissue Engineering. Part C, Methods, 1–13.Google Scholar
  88. 88.
    Wüst, S., Godla, M. E., Müller, R., & Hofmann, S. (2014). Tunable hydrogel composite with two-step processing in combination with innovative hardware upgrade for cell-based three-dimensional bioprinting. Acta Biomaterialia, 10(2), 630–640.Google Scholar
  89. 89.
    J. Chiang, M. Lehmicke, D. Dcosta, X. Hu, F. Lin, and W. Sun, Rapid prototyping assisted design and development of inter-vertebral implants. Magnetic Resonance Imaging, no. 1, pp. 527–538.Google Scholar
  90. 90.
    Hong, S., et al. (2013). Cellular behavior in micropatterned hydrogels by bioprinting system depended on the cell types and cellular interaction. Journal of Bioscience and Bioengineering, 116(2), 224–230.Google Scholar
  91. 91.
    Kao, C.-T., Lin, C.-C., Chen, Y.-W., Yeh, C.-H., Fang, H.-Y., & Shie, M.-Y. (2015). Poly(dopamine) coating of 3D printed poly(lactic acid) scaffolds for bone tissue engineering. Materials Science and Engineering: C, 56, 165–173.Google Scholar
  92. 92.
    Serra, T., Planell, J. A., & Navarro, M. (2013). High-resolution PLA-based composite scaffolds via 3-D printing technology. Acta Biomaterialia, 9(3), 5521–5530.Google Scholar
  93. 93.
    Mohanty, S., et al. (2016). Fabrication of scalable tissue engineering scaffolds with dual-pore microarchitecture by combining 3D printing and particle leaching. Materials Science and Engineering: C, 61, 180–189.Google Scholar
  94. 94.
    Neill, P. F. O., Ben Azouz, A., Vázquez, M., Liu, J., Marczak, S., and Slouka, Z. (2014). Advances in three-dimensional rapid prototyping of microfluidic devices for biological applications. pp. 1–22.Google Scholar
  95. 95.
    Gross, B., Lockwood, S. Y., and Spence, D. M. (2016) Recent advances in analytical chemistry by 3D printing. Analytical Chemistry, p. Acs.Analchem.6b04344.Google Scholar
  96. 96.
    Clare, A. T., Chalker, P., Sutcliffe, C. J., Sutcliffe, C. J., and Tsopanos, Æ. S. (2008). Selective laser melting of high aspect ratio 3D nickel–titanium structures for MEMS applications for MEMS applications.Google Scholar
  97. 97.
    Duarte, L. C., Chagas, C. L. S., Ribeiro, L. E. B., and Coltro, W. K. T. (2017). 3D printing of microfluidic devices with embedded sensing electrodes for generating and measuring the size of microdroplets based on contactless conductivity detection. Sensors and Actuators B: Chemical.Google Scholar
  98. 98.
    Postiglione, G., Alberini, M., Leigh, S., Levi, M., and Turri, S. (2017). Effect of 3D-printed microvascular network design on the self-healing behavior of cross-linked polymers. ACS Applied Materials & Interfaces, p. Acsami.7b01830.Google Scholar
  99. 99.
    Road, M., Eh, E., M. R. C. S. Centre, R. Medicine, and L. France. Derived ECM scaffold for liver tissue engineering: a novel drug induced hybrid electrospun PCL-cell, pp. 1–38.Google Scholar
  100. 100.
    Bissoyi, A., Bit, A., Singh, B. K., Singh, A. K., Patra, P. K. Enhanced cryopreservation of MSCs in microfluidic bioreactor by regulated shear flow. Scientific Reports 6:35416.Google Scholar
  101. 101.
    Bissoyi, A., Kumar, A., Rizvanov, A. A., Nesmelov, A., Gusev, O., Patra, P. K., and Bit, A. Recent advances and future direction in lyophilisation and desiccation of mesenchymal stem cells. Stem Cells International 2016:3604203, 9 pages.Google Scholar
  102. 102.
    Bit, A., Bissoyi, A., Sinha, S. K., Patra, P. K., Saha, S. The inhibition of bio-film formation by graphene-modified stainless steel and titanium alloy for the treatment of periprosthetic infection: a comparative study. Biomedical Engineering Conference (SBEC), 2016 32nd Southern.  https://doi.org/10.1109/SBEC.2016.75.
  103. 103.
    Bissoyi, A., Singh, A. K., Pattanayak, S. K., Bit, A., Sinha, S. K., Patel, A., Jain, V., Patra, P. K.. Understanding molecular mechanism of improved proliferation and osteogenic potential of human mesenchymal stem cells grown on polyelectrolyte complex derived from non-mulberry silk fibroin and chitosan. Biomedical Materials, Accepted Manuscript online 30 August 2017.Google Scholar
  104. 104.
    Bit, A., Kumar, A., Singh, A. K., Rizvanov, A. A., Kiassov, A. P., Patra, P. K., Kumar, M., & Bissoyi, A. (2017). Crosstalk between substrates and rho-associated kinase inhibitors in cryopreservation of tissue-engineered constructs. Stem Cells International, 2017, 1380304 9 pages.Google Scholar
  105. 105.
    Singh, A. K., Jha, A., Bit, A., Kiassov, A. P., Rizvanov, A. A., Ojha, A., Bhoi, P., Patra, P. K., Kumar, A., Bissoyi, A. Selaginella bryopteris aqueous extract improves stability and function of cryopreserved human mesenchymal stem cells. Oxidative Medicine and Cellular Longevity, 2017:8530656, 10 pages.Google Scholar
  106. 106.
    Nie, W., et al. (2017). Three-dimensional porous scaffold by self-assembly of reduced graphene oxide and nano-hydroxyapatite composites for bone tissue engineering. Carbon New York, 116, 325–337.Google Scholar

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Authors and Affiliations

  1. 1.National Institute of TechnologyRaipurIndia

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