Advertisement

BioNanoScience

, Volume 6, Issue 4, pp 523–527 | Cite as

Impairments to the Giant Depolarizing Potentials After the Third Trimester Equivalent Ethanol Exposure in the Neonatal Rat

  • Andrey Zakharov
  • Nailya Lotfullina
  • Roustem Khazipov
Article

Abstract

Ethanol exerts multiple adverse effects in the developing hippocampus resulting in the life-long neurological and behavioral deficits. However, the early disturbances in the hippocampal network function after exposure to ethanol remain largely unknown. Here, we examined the properties of the neonatal CA3 hippocampal network-driven giant depolarizing potentials using extracellular recordings of the local field potential and multiple units from the hippocampal slices prepared from the 5–11-day-old rats treated by ethanol in vivo (6 g/kg, intraperitoneally), 12 h before the slice preparation. Activity in hippocampal slices from the ethanol-treated animals displayed several abnormalities including a threefold increase in overall neuronal firing and profound, by nearly sevenfold, reduction of synchronization of CA3 units in giant depolarizing potentials. Thus, alterations in the hippocampal network function emerge shortly after the ethanol exposure and manifest in the enhanced excitation and severe impairments to the giant depolarizing potentials.

Keywords

Alcoholism Fetal alcohol syndrome Hippocampus Giant depolarizing potentials Neonate 

Notes

Acknowledgments

This work was supported by INSERM (LIA to RK), the Program of Competitive Growth of Kazan Federal University and the subsidy allocated to Kazan Federal University for the state assignment in the sphere of scientific activities.

Compliance with Ethical Standards

This work has been carried out in accordance with EU Directive 2010/63/EU for animal experiments and all the protocols were approved by INSERM (N007.08.01) and KSMU (N9-2013).

References

  1. 1.
    Ikonomidou, C., Bittigau, P., Ishimaru, M. J., Wozniak, D. F., Koch, C., Genz, K., et al. (2000). Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome. Science, 287, 1056–1060.CrossRefGoogle Scholar
  2. 2.
    Olney, John W. (2014) Focus on apoptosis to decipher how alcohol and many other drugs disrupt brain development. Frontiers in Pediatrics, 2(81), 1–3.Google Scholar
  3. 3.
    Krawczyk, M., Ramani, M., Dian, J., Florez, C. M., Mylvaganam, S., Brien, J., et al. (2016). Hippocampal hyperexcitability in fetal alcohol spectrum disorder: pathological sharp waves and excitatory/inhibitory synaptic imbalance. Experimental Neurology, 280, 70–79.CrossRefGoogle Scholar
  4. 4.
    Ben-Ari, Y., Cherubini, E., Corradetti, R., Gaïarsa, J.-L. (1989). Giant synaptic potentials in immature rat CA3 hippocampal neurones. Journal of Physiology (London), 416, 303–325.CrossRefGoogle Scholar
  5. 5.
    Khazipov, R., Leinekugel, X., Khalilov, I., Gaïarsa, J.-L., Ben-Ari, Y. (1997). Synchronization of GABAergic interneuronal network in CA3 subfield of neonatal rat hippocampal slices. Journal of Physiology (London), 498, 763–772.CrossRefGoogle Scholar
  6. 6.
    Khazipov, R., Khalilov, I., Tyzio, R., Morozova, E., Ben Ari, Y., Holmes, G. L. (2004). Developmental changes in GABAergic actions and seizure susceptibility in the rat hippocampus. European Journal of Neuroscience, 19, 590–600.CrossRefGoogle Scholar
  7. 7.
    Khalilov, I., Minlebaev, M., Mukhtarov, M., Khazipov, R. (2015). Dynamic changes from depolarizing to hyperpolarizing GABAergic actions during giant depolarizing potentials in the neonatal rat hippocampus. Journal of Neuroscience, 35, 12635–12642.CrossRefGoogle Scholar
  8. 8.
    Everett, J. C., Licon-Munoz, Y., Valenzuela, C. F. (2012). Effects of third trimester-equivalent ethanol exposure on Cl(-) co-transporter expression, network activity, and GABAergic transmission in the CA3 hippocampal region of neonatal rats. Alcohol, 46, 595–601.CrossRefGoogle Scholar
  9. 9.
    Galindo, R., & Valenzuela, C. F. (2006). Immature hippocampal neuronal networks do not develop tolerance to the excitatory actions of ethanol. Alcohol, 40, 111–118.CrossRefGoogle Scholar
  10. 10.
    Tsintsadze, V., Minlebaev, M., Suchkov, D., Cunningham, M. O., and Khazipov, R. (2015) Ontogeny of kainate-induced gamma oscillations in the rat CA3 hippocampus in vitro. Frontiers in Cellular Neuroscience, 9(195), 1–15.Google Scholar
  11. 11.
    Khalilov, I., Dzhala, V., Ben-Ari, Y., Khazipov, R. (1999). Dual role of GABA in the neonatal rat hippocampus. Developmental Neuroscience, 21, 310–319.CrossRefGoogle Scholar
  12. 12.
    Valeeva, G., Abdullin, A., Tyzio, R., Skorinkin, A., Nikolski, E., Ben-Ari, Y., and Khazipov, R. (2010) Temporal coding at the immature depolarizing GABAergic synapse. Frontiers in Cellular Neuroscience, 4(17), 1–12.Google Scholar
  13. 13.
    Lebedeva, J., Zakharov, A., Ogievetsky, E., Minlebaeva, A., Kurbanov, R., Gerasimova, E., Sitdikova, G., and Khazipov, R. (2015) Inhibition of cortical activity and apoptosis caused by ethanol in neonatal rats in vivo. Cereb Cortex (epub ahead print).Google Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Andrey Zakharov
    • 1
    • 2
  • Nailya Lotfullina
    • 1
    • 3
    • 4
  • Roustem Khazipov
    • 1
    • 3
    • 4
  1. 1.Laboratory of NeurobiologyKazan Federal UniversityKazanRussia
  2. 2.Department of PhysiologyKazan State Medical UniversityKazanRussia
  3. 3.INMED - INSERM U901MarseilleFrance
  4. 4.Aix-Marseille UniversityMarseilleFrance

Personalised recommendations