Development of a Colloidal Gold-Based Nanobioprobe for the Detection of Glycated Albumin
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A simple, inexpensive, and quick colorimetric immunoassay for the determination of diabetic biomarker (glycated albumin) using antibody labeled gold nanoparticles is reported. The sensitivity of the assay on the basis of absorbance measurements is excellent with a detection limit of 1 μg/ml with 40 nm sized gold nanoparticles. The assay is free of washing cycle and result can be read in minutes. Also, the method requires very low amount of the sample as compared to the conventional immunossays. Therefore, this method can be used for the simple, low cost, and rapid detection of diabetes marker, glycated albumin.
KeywordsImmunoassay Gold nanoparticles Glycated albumin Diabetes
This work is supported by INSPIRE Faculty award [IFA12-CH-52] by the Department of Science and Technology (DST), New Delhi, India, to Dr. Nishima Wangoo. Some experiments were performed in the EPIC and NIFTI facility of NUANCE center at Northwestern University, which is supported by NSF-NSEC, NSF-MRSEC, Keck Foundation, the State of Illinois, and Northwestern University. The authors would like to thank Dr. Gajendra Shekhawat, Dr Soo Hyun Tark, and Prof. Vinayak Dravid from Northwestern University for the same. Scientific discussions and expert guidance from Dr. C Raman Suri from IMTECH, Chandigarh, India, are also duly and highly acknowledged.
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