, Volume 5, Issue 3, pp 135–139 | Cite as

Green Synthesis of Copper Oxide Nanoparticles Using Aloe vera Leaf Extract and Its Antibacterial Activity Against Fish Bacterial Pathogens

  • P. P. N. Vijay Kumar
  • U. Shameem
  • Pratap Kollu
  • R. L. Kalyani
  • S. V. N. Pammi


The present study reports biologically oriented process for green synthesis of CuO nanoparticles by using eco-friendly and non-toxic Aloe vera leaf extract. Powder X-ray diffraction and transmission electron microscope analysis revealed that synthesized CuO nanoparticles are in monoclinic phase with average particle size of 20 nm. The antibacterial activity of green synthesized CuO nanoparticles was tested against three bacterial fish pathogens “viz:” Aeromonas hydrophila, Pseudomonas fluorescens and Flavobacterium branchiophilum, which are responsible for causing severe infectious diseases in fishes. CuO NPs exhibits enhanced antibacterial activity against all the fish pathogens even at lower concentrations, i.e. above 20 μg/mL.


Green synthesis Fish pathogens Aloe vera Leaf extract Antibacterial activity 



We are thankful to the DST-PURSE Programme, Advanced Analytical Laboratory, Andhra University, for the financial assistance and for their support in carrying out the research work regarding SEM-EDX and XRD analysis. The authors are thankful to CRNTS, IIT Bombay for the TEM characterization.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • P. P. N. Vijay Kumar
    • 1
    • 3
  • U. Shameem
    • 1
  • Pratap Kollu
    • 2
  • R. L. Kalyani
    • 4
  • S. V. N. Pammi
    • 3
  1. 1.Department of Zoology, College of Science and TechnologyAndhra UniversityVisakhapatnamIndia
  2. 2.DST-INSPIRE Faculty, Department of Metallurgical Engineering & Materials ScienceIndian Institute of Technology BombayMumbaiIndia
  3. 3.Advanced Analytical Laboratory, DST-PURSE ProgrammeAndhra UniversityVisakhapatnamIndia
  4. 4.Sri Vishnu College of Pharmacy, VishnupurBhimavaramIndia

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