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BioNanoScience

, Volume 3, Issue 1, pp 21–29 | Cite as

Tripeptide Self-Assembled Hydrogels: Soft Nanomaterials for Biological Applications

  • Silvia MarchesanEmail author
  • Lynne Waddington
  • Christopher D. Easton
  • Firdawosia Kushkaki
  • Keith M. McLean
  • John S. Forsythe
  • Patrick G. Hartley
Article

Abstract

Short peptide self-assembled hydrogels are a promising class of soft nanomaterials for drug delivery and regenerative medicine. Here we describe the gelation of tripeptides, consisting of d and l hydrophobic amino acids, in buffer, following a pH switch to 7.4 (i.e., physiological pH). Interestingly, tripeptide analogues consisting of l-only amino acids fail to form a gel under the same conditions. The nanostructure of these self-assembling peptides is investigated by a number of techniques (atomic force microscopy, transmission electron microscopy, and confocal light microscopy) to unveil their architectures. In addition, these self-assembled soft nanomaterials can be potentially used as vehicles to deliver bioactive molecules. For instance, we describe the incorporation into the gel of rhodamine dye as a model compound in a two-step procedure: firstly, the dye is dissolved in the gel precursor solution; secondly, dilution to a final pH of 7.4 triggers self-assembly and gelation of the system. We analyse the effect of dye incorporation within either precursor solution on the secondary structure, nanoarchitecture, and rheological properties of the resulting peptide materials. We also present data concerning dye release kinetics. Dye release is achieved within 48 h, and no burst release is observed. We anticipate that these systems will find biological applications for the delivery of bioactive compounds.

Keywords

Peptide self-assembly Hydrogel Release Biomaterials 

Notes

Acknowledgments

The authors acknowledge the facilities of Monash Micro Imaging, Monash University, Australia, and in particular Stephen Firth, Dr. Judy Callaghan and Dr. Alex Fulcher for their scientific and technical assistance. The authors also acknowledge the CSIRO-Monash University Collaborative Research Support Scheme (CRSS) for funding.

Supplementary material

12668_2012_74_MOESM1_ESM.doc (15.6 mb)
ESM 1 DOC 15.5 mb
ESM 2

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Silvia Marchesan
    • 1
    Email author
  • Lynne Waddington
    • 1
  • Christopher D. Easton
    • 1
  • Firdawosia Kushkaki
    • 2
  • Keith M. McLean
    • 1
  • John S. Forsythe
    • 3
  • Patrick G. Hartley
    • 1
  1. 1.CSIRO Materials Science and EngineeringClaytonAustralia
  2. 2.Department of ChemistryLa Trobe UniversityBundooraAustralia
  3. 3.Department of Materials EngineeringMonash UniversityClaytonAustralia

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