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BioNanoScience

, Volume 2, Issue 4, pp 185–195 | Cite as

Multiwalled Carbon Nanotubes for Amperometric Array-Based Biosensors

  • Irene Taurino
  • Giovanni De Micheli
  • Sandro Carrara
Article

Abstract

For diagnostic and therapeutic purposes an accurate determination of multiple metabolites is often required. Amperometric devices are attractive tools to quantify biological compounds due to the direct conversion of a biochemical event to a current. This review addresses recent developments in the use of multiwalled carbon nanotubes to enhance detection capability of amperometric array-based biosensors. More specifically, the principal techniques for multiwalled carbon nanotube incorporation onto microelectrode arrays are described. In these types of devices, each electrode is responsible for sensing one metabolite. The specificity is often given by an enzyme since most biomolecules are not electroactive compounds. Common strategies for the protein immobilization onto multiwalled carbon nanotubes are also presented. After the discussion of nanotube/biomolecule integration onto electrode surfaces, three results are shown. The first one regards the influence on the biodetection signal of differently oriented multiwalled carbon nanotubes. Secondly, a demonstration of enhanced biodetection parameters by using multiwalled carbon nanotubes is given. Finally, a comparative study of three enzymes used to detect the same metabolite and adsorbed onto multiwalled carbon nanotubes is also reported.

Keywords

Multiwalled carbon nanotubes Metabolite detection Amperometric array-based biosensors Enzyme immobilization Nanostructure integration 

Notes

Acknowledgements

The authors would like to thank Alberto Tagliaferro e Mauro Giorcelli for the MWCNTs fabrications and Linda Thöny-Meyer for providing NLox and wtLox. The research is supported by the i-IronIC project. The i-IronIC project is financed with a grant from the Swiss Nano-Tera.ch initiative and evaluated by the Swiss National Science Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Irene Taurino
    • 1
  • Giovanni De Micheli
    • 1
  • Sandro Carrara
    • 1
  1. 1.Laboratory of Integrated SystemsEPFL - École Polytechnique Fédérale de LausanneLausanneSwitzerland

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