, Volume 1, Issue 4, pp 123–127 | Cite as

Tropoelastin Switch and Modulated Endothelial Cell Binding to PTFE

  • Daniel V. Bax
  • Siyuan John Liu
  • David R. McKenzie
  • Marcela M. M. Bilek
  • Anthony S. WeissEmail author

To the Editor:

Polytetrafluoroethylene (PTFE) is routinely modified and used for the fabrication of vascular conduits. In its untreated form, it displays low cell-binding activity which combined with persistent thrombogenicity limits its utility. Full endothelial cell coverage of the blood-contacting surface would present a more natural lumen interface and is likely reduce the thrombogenicity of vascular grafts. Thrombosis is the major cause of short-term failure of heart valves, coronary stents, and small-diameter synthetic grafts. The ECM protein tropoelastin, the precursor of elastin, has a role in signaling and regulating luminal endothelial cells in the arterial wall. Additionally, elastin possesses low thrombogenicity and is being assessed for its potential as a vascular conduit component [1]. Here, we explore the potential of coating polymers with tropoelastin to control endothelial cell interactions with the eventual goal of bestowing low thrombogenicity. There is a paucity of...


PTFE Heparan Sulfate Vascular Graft Human Dermal Fibroblast Endothelial Cell Interaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We gratefully acknowledge project funding from the Australian Research Council and the National Health & Medical Research Council. We thank Cochlear Ltd. and SpineCell Pty Ltd for financial and in-kind support. We thank Daniel Smyth for assistance and Yan Yee Poon, Rodrigo Vazquez-Lombardi, and Sarah Martinez for the technical assistance.


  1. 1.
    Waterhouse, A., Wise, S. G., Ng, M. K., & Weiss, A. S. (2011). Elastin as a nonthrombogenic biomaterial. Tissue Engineering. Part B, Reviews, 17(2), 93–99.CrossRefGoogle Scholar
  2. 2.
    Tzoneva, R., Faucheux, N., & Groth, T. (2007). Wettability of substrata controls cell-substrate and cell-cell adhesions. Biochimica et Biophysica Acta, 1770(11), 1538–1547.Google Scholar
  3. 3.
    Garcia, A. J. (2005). Get a grip: integrins in cell-biomaterial interactions. Biomaterials, 26(36), 7525–7529.CrossRefGoogle Scholar
  4. 4.
    Bacakova, L., Filova, E., Rypacek, F., Svorcik, V., & Stary, V. (2004). Cell adhesion on artificial materials for tissue engineering. Physiological Research, 53(Suppl 1), S35–45.Google Scholar
  5. 5.
    Vermeer, A. W., Bremer, M. G., & Norde, W. (1998). Structural changes of IgG induced by heat treatment and by adsorption onto a hydrophobic teflon surface studied by circular dichroism spectroscopy. Biochimica et Biophysica Acta, 1425(1), 1–12.Google Scholar
  6. 6.
    Mollmann, S. H., et al. (2006). Interfacial adsorption of insulin conformational changes and reversibility of adsorption. European Journal of Pharmaceutical Sciences, 27(2–3), 194–204.CrossRefGoogle Scholar
  7. 7.
    Sethuraman, A., Vedantham, G., Imoto, T., Przybycien, T., & Belfort, G. (2004). Protein unfolding at interfaces: slow dynamics of alpha-helix to beta-sheet transition. Proteins, 56(4), 669–678.CrossRefGoogle Scholar
  8. 8.
    Zardeneta, G., Mukai, H., Marker, V., & Milam, S. B. (1996). Protein interactions with particulate Teflon: implications for the foreign body response. Journal of Oral and Maxillofacial Surgery, 54(7), 873–878.CrossRefGoogle Scholar
  9. 9.
    Balasubramanian, V., Grusin, N. K., Bucher, R. W., Turitto, V. T., & Slack, S. M. (1999). Residence-time dependent changes in fibrinogen adsorbed to polymeric biomaterials. Journal of Biomedical Materials Research, 44(3), 253–260.CrossRefGoogle Scholar
  10. 10.
    Bilek, M. M. M., & McKenzie, D. R. (2010). Plasma modified surfaces for covalent immobilization of functional biomolecules in the absence of chemical linkers: towards better biosensors and a new generation of medical implants. Biophysical Reviews, 2, 55–65.CrossRefGoogle Scholar
  11. 11.
    Bax, D. V., et al. (2011). Binding of the cell adhesive protein tropoelastin to PTFE through plasma immersion ion implantation treatment. Biomaterials, 32, 5100–5011.CrossRefGoogle Scholar
  12. 12.
    Bax DV, McKenzie DR, Bilek MMM, Weiss AS (2011) Directed cell attachment by tropoelastin on masked plasma immersion ion implantation treated PTFE. Biomaterials accepted manuscript.Google Scholar
  13. 13.
    Wilson, B. D., et al. (2011). Novel approach for endothelializing vascular devices: understanding and exploiting elastin-endothelial interactions. Annals of Biomedical Engineering, 39(1), 337–346.CrossRefGoogle Scholar
  14. 14.
    Bax, D. V., Rodgers, U. R., Bilek, M. M. M., & Weiss, A. S. (2009). Cell adhesion to tropoelastin is mediated via the C-terminal GRKRK motif and integrin alpha(V)beta(3). Journal of Biological Chemistry, 284(42), 28616–28623.CrossRefGoogle Scholar
  15. 15.
    Akhtar, K., et al. (2011). Oxidative modifications of the C-terminal domain of tropoelastin prevent cell binding. Journal of Biological Chemistry, 286(15), 13574–13582.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Daniel V. Bax
    • 1
    • 2
  • Siyuan John Liu
    • 3
  • David R. McKenzie
    • 1
  • Marcela M. M. Bilek
    • 1
  • Anthony S. Weiss
    • 2
    Email author
  1. 1.Applied and Plasma Physics, School of PhysicsUniversity of SydneySydneyAustralia
  2. 2.School of Molecular BioscienceUniversity of SydneySydneyAustralia
  3. 3.School of Medicine, University of California San FranciscoSan FranciscoUSA

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