Tropoelastin Switch and Modulated Endothelial Cell Binding to PTFE
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To the Editor:
Polytetrafluoroethylene (PTFE) is routinely modified and used for the fabrication of vascular conduits. In its untreated form, it displays low cell-binding activity which combined with persistent thrombogenicity limits its utility. Full endothelial cell coverage of the blood-contacting surface would present a more natural lumen interface and is likely reduce the thrombogenicity of vascular grafts. Thrombosis is the major cause of short-term failure of heart valves, coronary stents, and small-diameter synthetic grafts. The ECM protein tropoelastin, the precursor of elastin, has a role in signaling and regulating luminal endothelial cells in the arterial wall. Additionally, elastin possesses low thrombogenicity and is being assessed for its potential as a vascular conduit component . Here, we explore the potential of coating polymers with tropoelastin to control endothelial cell interactions with the eventual goal of bestowing low thrombogenicity. There is a paucity of...
KeywordsPTFE Heparan Sulfate Vascular Graft Human Dermal Fibroblast Endothelial Cell Interaction
We gratefully acknowledge project funding from the Australian Research Council and the National Health & Medical Research Council. We thank Cochlear Ltd. and SpineCell Pty Ltd for financial and in-kind support. We thank Daniel Smyth for assistance and Yan Yee Poon, Rodrigo Vazquez-Lombardi, and Sarah Martinez for the technical assistance.
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