Environmental Earth Sciences

, Volume 74, Issue 6, pp 5211–5219 | Cite as

Enhanced in situ bioremediation of groundwater contaminated by petroleum hydrocarbons at the location of the Nitex textiles, Serbia

  • Nenad MarićEmail author
  • Mila Ilić
  • Srđan Miletić
  • Gordana Gojgić-Cvijović
  • Vladimir Beškoski
  • Miroslav M. Vrvić
  • Petar Papić
Original Article


This study provides insight into the enhanced in situ bioremediation applied for remediation of groundwater contaminated by petroleum hydrocarbons. Activities prior to the application of this remediation approach included removal of the source of groundwater contamination—an underground storage tank and contaminated sediments—from the unsaturated zone. The hydraulic feasibility of this remediation approach was proved by hydraulic conductivity characterization of the site. Enhanced in situ bioremediation was performed by a combination of biostimulation and bioaugmentation within the closed bipolar system (one extraction and two injection wells). Biostimulation was conducted by addition of nutrients, and stimulation of oxidation processes by injection of H2O2, into the aquifer. Bioaugmentation/re-inoculation was achieved by injection of a zymogenous consortium of hydrocarbon degrading microorganisms isolated from the contaminated groundwater. The average extraction capacity was 0.5 l/s, with an average injection capacity of 0.25 l/s per well. The efficiency of the remediation approach was measured by changes in the content of total petroleum hydrocarbon (TPH), total chemoorganoheterotrophic (TC) and hydrocarbon degrading (HD) microorganisms. After biostimulation and bioaugmentation, the number of TC and HD microorganisms started to increase. This was followed by a decrease of TPH concentration in the groundwater from an initial 6.8–0.5 mg/l at the end of the bioremediation. The applied remediation approach was highly efficient and very effective in reducing TPH to acceptable levels. Together, these facts provide strong evidence of its potential for remediation of groundwater contaminated by petroleum hydrocarbons.


Groundwater Petroleum hydrocarbons Bioremediation In situ 



This study was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia under Grant No. 43004.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Nenad Marić
    • 1
    Email author
  • Mila Ilić
    • 3
  • Srđan Miletić
    • 3
  • Gordana Gojgić-Cvijović
    • 3
  • Vladimir Beškoski
    • 2
  • Miroslav M. Vrvić
    • 2
  • Petar Papić
    • 1
  1. 1.Faculty of Mining and GeologyUniversity of BelgradeBelgradeSerbia
  2. 2.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia
  3. 3.Center of Chemistry, IChTMUniversity of BelgradeBelgradeSerbia

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