Environmental Earth Sciences

, Volume 73, Issue 4, pp 1473–1477 | Cite as

Dissolved and gaseous inorganic carbon sequestration using a close system cell-free ureolytic calcification process

Original Article
First Online:
Received:
Accepted:

Abstract

Ureolysis mediated increase of culture medium pH, dissolved inorganic carbon (DIC) by Bacillus pasteurii and subsequent utilization of the cell-free broth on calcification mediated carbon (CO2) capture was investigated. In an ureolytic process with an initial biomass of 80 mg/l, about 55 % of the original urea (100 mM) in the nutrient broth-NaCl medium was hydrolyzed over 24 h; this increased DIC to 1,145 mg/l and pH to 9.1. The gas phase CO2 capture experiment, which used the culture medium supernatant in a closed system (liquid phase to headspace volume ratio of 1:3) containing 28.45 % v/v headspace CO2 (g), was triggered by injecting 50 mM CaCl2. The calcification process decreased both the headspace CO2 and the liquid phase DIC to 18.48 % v/v and 942 mg/l, respectively, through precipitation of total 27.41 mM CaCO3. Although only 21.8 % of the DIC input from ureolysis was actually captured as solid carbonate, solubility trapping (as soluble carbonate in DIC) effectively regulated CO2 release into the atmosphere.

Keywords

Bacillus pasteurii Carbon dioxide capture Dissolved inorganic carbon Solubility trapping Ureolytic calcification 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgments

This research was financially supported by NRF project of development of biomimetic technology for acid soil rehabilitation using biocalcification process and partially by INHA University Research Grant.

References

  1. APHA (2005) Standard methods for the examination of water & wastewater. American Public Health Association (APHA)Google Scholar
  2. Assayag N, Rivé K, Ader M et al (2006) Improved method for isotopic and quantitative analysis of dissolved inorganic carbon in natural water samples. Rapid Commun Mass Spectrom 20:2243–2251. doi: 10.1002/rcm.2585 CrossRefGoogle Scholar
  3. Becker V, Myrttinen A, Blum P et al (2011) Predicting δ13CDIC dynamics in CCS: a scheme based on a review of inorganic carbon chemistry under elevated pressures and temperatures. Int J Greenh Gas Control 5:1250–1258. doi: 10.1016/j.ijggc.2011.05.001 CrossRefGoogle Scholar
  4. Chou C-W, Seagren EA, Aydilek AH, Lai M (2011) Biocalcification of sand through ureolysis. J Geotech Geoenviron Eng 137:1179–1189. doi: 10.1061/(ASCE)GT.1943-5606.0000532 CrossRefGoogle Scholar
  5. De Vrind-de Jong EW, de Vrind JPM (1997) Algal deposition of carbonates and silicates. Rev Mineral Geochem 35:267–307Google Scholar
  6. Dittrich M, Müller B, Mavrocordatos D, Wehrli B (2003) Induced calcite precipitation by cyanobacterium Synechococcus. Acta Hydrochim Hydrobiol 31:162–169. doi: 10.1002/aheh.200300486 CrossRefGoogle Scholar
  7. Dittrich M, Kurz P, Wehrli B (2004) The Role of autotrophic Picocyanobacteria in calcite precipitation in an oligotrophic lake. Geomicrobiol J 21:45–53. doi: 10.1080/01490450490253455 CrossRefGoogle Scholar
  8. Doney SC, Fabry VJ, Feely RA, Kleypas JA (2009) Ocean acidification: the other CO2 problem. Ann Rev Mar Sci 1:169–192. doi: 10.1146/annurev.marine.010908.163834 CrossRefGoogle Scholar
  9. Ferris FG, Phoenix V, Fujita Y, Smith RW (2004) Kinetics of calcite precipitation induced by ureolytic bacteria at 10 to 20 °C in artificial groundwater. Geochim Cosmochim Acta 68:1701–1710CrossRefGoogle Scholar
  10. Fujita Y, Ferris FG, Lawson RD et al (2000) Calcium carbonate precipitation by ureolytic subsurface bacteria. Geomicrobiol J 17:305–318. doi: 10.1080/782198884 CrossRefGoogle Scholar
  11. Hammes F, Verstraete W (2002) Key roles of pH and calcium metabolism in microbial carbonate precipitation. Rev Environ Sci Biotechnol 1:3–7. doi: 10.1023/A:1015135629155 CrossRefGoogle Scholar
  12. Langdon C, Broecker WS, Hammond DE et al (2003) Effect of elevated CO2 on the community metabolism of an experimental coral reef. Global Biogeochem Cycles 17:1011. doi: 10.1029/2002GB001941 CrossRefGoogle Scholar
  13. Lee BD, Apel WA, Walton MR (2004) Screening of cyanobacterial species for calcification. Biotechnol Prog 20:1345–1351. doi: 10.1021/bp0343561 CrossRefGoogle Scholar
  14. Lim M, Han G-C, Ahn J-W, You K-S (2010) Environmental remediation and conversion of carbon dioxide (CO2) into useful green products by accelerated carbonation technology. Int J Environ Res Public Health 7:203–228. doi: 10.3390/ijerph7010203 CrossRefGoogle Scholar
  15. Mahanty B, Kim S, Kim CG (2014) Dissolved inorganic carbon inventory and CO2 sequestration in ureolytic biocalcification process with Bacillus pasteurii. Geomicrobiol J 31:145–151. doi: 10.1080/01490451.2013.819051 CrossRefGoogle Scholar
  16. Mitchell AC, Ferris FG (2005) The coprecipitation of Sr into calcite precipitates induced by bacterial ureolysis in artificial groundwater: temperature and kinetic dependence. Geochim Cosmochim Acta 69:4199–4210CrossRefGoogle Scholar
  17. Mitchell AC, Dideriksen K, Spangler LH et al (2010) Microbially enhanced carbon capture and storage by mineral-trapping and solubility-trapping. Environ Sci Technol 44:5270–5276. doi: 10.1021/es903270w CrossRefGoogle Scholar
  18. Sarda D, Choonia HS, Sarode DD, Lele SS (2009) Biocalcification by Bacillus pasteurii urease: a novel application. J Ind Microbiol Biotechnol 36:1111–1115. doi: 10.1007/s10295-009-0581-4 CrossRefGoogle Scholar
  19. Solomon S, Qin D, Manning M et al. (2007) Climate change 2007: the physical science basis: contribution of working group I to the fourth assessment report of the intergovernmental panel on climate change, p 996Google Scholar
  20. Tyagi C, Tomar LK, Singh H (2009) Surface modification of cellulose filter paper by glycidyl methacrylate grafting for biomolecule immobilization: influence of grafting parameters and urease immobilization. J Appl Polym Sci 111:1381–1390. doi: 10.1002/app.28933 CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of Environmental EngineeringINHA UniversityIncheonRepublic of Korea

Personalised recommendations