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Indian Journal of Gastroenterology

, Volume 38, Issue 2, pp 110–116 | Cite as

Genotype distribution in relation to viral load in a large cohort of Indian patients with chronic hepatitis C virus infection: A retrospective analysis

  • Balkumar Reddy Panyala
  • Rathindra Mohan MukherjeeEmail author
  • Himaja Devarakonda
  • Sivasathish Tadivaka
  • Nagaraja Rao Padaki
  • Mithun Sharma
  • Nageshwar Reddy Duvvuru
Original Article
  • 83 Downloads

Abstract

Introduction

Hepatitis C virus (HCV) displays high genetic diversity, characterized by regional variations in the prevalence of genotype posing challenges to the development of vaccines and definitive treatment. Very few reports exist on the distribution and frequency change of HCV genotypes in India. In the present retrospective study, we aimed to understand the distribution pattern of HCV genotypes and viral load among HCV-infected patients attending the Asian Institute of Gastroenterology, Hyderabad, India, a tertiary care hospital.

Methods

Patients referred to the Hepatology Department from January 2009 to December 2015 were screened for this study. Eight hundred and sixty-two chronic HCV patients were included in this study. Genotyping was performed using type-specific probe-based hybridization assay and viral load was estimated by real-time polymerase chain reaction.

Results

Out of 862 patients, genotype 1 was detected predominantly in 392 (45.5%), followed by genotype 3 in 344 (39.9%) patients; genotypes 4, 6, and 2 were detected in 115 (13.3%), 8 (0.9%), and 3 (0.3%) patients, respectively. The number of patients having genotype 1 increased in frequency while genotype 3 became less from the year 2009 to 2015. Patients having genotype 1 had significantly (p < 0.0001) higher viral load compared with the patients infected with other genotypes.

Conclusion

Our study results demonstrate a change in HCV genotypic distribution pattern from genotypes 3 to 1 during the span of 7 years in patients referred to our hospital. In the light of the reported difference in the pathogenic potential of various HCV genotypes, detection of HCV genotype appears to be still essential for better patient management.

Keywords

HCV genotyping Hepatitis C virus Viral RNA 

Notes

Compliance with ethical standards

Conflict of interest

BRP, RMM, HD, ST, NRP, MS, and NRD declares that there are no conflicts of interest. The study protocol was approved by the Institutional Review Board.

Ethics statement

The authors declare that the study was performed in a manner conforming to the Helsinki declaration of 1975, as revised in 2000 and 2008 concerning human and animal rights, and the authors followed the policy concerning informed consent as shown on Springer.com.

Disclaimer

The authors are solely responsible for the data and the content of the paper. In no way, the Honorary Editor-in-Chief, Editorial Board Members, or the printer/publishers are responsible for the results/findings and content of this article.

References

  1. 1.
    Lauer GM, Walker BD. Hepatitis C virus infection. N Engl J Med. 2001;345:41–52.CrossRefGoogle Scholar
  2. 2.
    Cooke GS, Lemoine M, Thursz M, et al. Viral hepatitis and the global burden of disease: a need to regroup. J Viral Hepat. 2013;20:600–1.CrossRefGoogle Scholar
  3. 3.
    Mohd HK, Groeger J, Flaxman AD, Wiersma ST. Global epidemiology of hepatitis C virus infection: new estimates of age specifc antibody to HCV seroprevalence. Hepatology. 2013;57:1333–42.CrossRefGoogle Scholar
  4. 4.
    Khaja MN, Munpally SK, Hussain MM, Habeebullah CM. Hepatitis C virus: the Indian scenario. Curr Sci. 2002;83:219–24.Google Scholar
  5. 5.
    Bostan N, Mahmood T. An overview about hepatitis C: a devastating virus. Crit Rev Microbiol. 2010;36:91–133.CrossRefGoogle Scholar
  6. 6.
    Timm J, Roggendorf M. Sequence diversity of hepatitis C virus: implications for immune control and therapy. World J Gastroenterol. 2007;13:4808–17.CrossRefGoogle Scholar
  7. 7.
    Jane PM, Isla H, Abraham F, et al. Global distribution and prevalence of hepatitis C virus genotypes. Hepatology. 2015;61:77–87.CrossRefGoogle Scholar
  8. 8.
    Jacobson IM, Gordon C, Kowdley KV, et al. Sofosbuvir for hepatitis C genotype 2 or 3 in patients without treatment options. N Engl J Med. 2013;368:1867–77.CrossRefGoogle Scholar
  9. 9.
    Lawitz E, Mangia A, Wyles D, et al. Sofosbuvir for previously untreated chronic hepatitis C infection. N Engl J Med. 2013;368:1878–87.CrossRefGoogle Scholar
  10. 10.
    Wedemeyer H. Towards interferon-free treatment for all HCV genotypes. Lancet. 2015;385:2443–5.CrossRefGoogle Scholar
  11. 11.
    Garcia-Montalvo BM, Galguera-Colorado PL. Distribution of hepatitis C virus genotypes, risk factors and liver disease in patients from Yucatan, Mexico. Ann Hepatol. 2008;7:345–9.CrossRefGoogle Scholar
  12. 12.
    Verma V, Chakravarti A, Kar P. Genotypic characterization of hepatitis C virus and its significance in patients with chronic liver disease from northern India. Diagn Microbiol Infect Dis. 2008;61:408–14.CrossRefGoogle Scholar
  13. 13.
    Chakravarti A, Verma V. Distribution of hepatitis genotypes in β-thalassaemic patients from North India. Trans Med. 2006;16:433–8.CrossRefGoogle Scholar
  14. 14.
    Panigrahi AK, Roca J, Acharya SK, Jameel S, Panda SK. Genotype determination of hepatitis C virus from northern India: identification of new subtypes. J Med Virol. 1996;48:191–8.CrossRefGoogle Scholar
  15. 15.
    Valliammai T, Thyagarajan SP, Zuckerman AJ, Harrisomn TJ. Diversity of genotypes of hepatitis C virus in southern India. J Gen Virol. 1995;76:711–6.CrossRefGoogle Scholar
  16. 16.
    Chowdhury A, Santra A, Chaudhuri S, et al. Hepatitis C virus in the general population: a community-based study in West Bengal, India. Hepatology. 2003;37:802–9.CrossRefGoogle Scholar
  17. 17.
    Raghuraman S, Shaji RV, Sridharan G, et al. Distribution of the different genotypes of HCV among patients attending a tertiary care hospital in south India. J Clin Virol. 2003;26:61–9.CrossRefGoogle Scholar
  18. 18.
    Ghany MG, Strader DB, Thomas DL, Seeff LB, American Association for the Study of Liver Diseases. Diagnosis, management, and treatment of hepatitis C: an update. Hepatology. 2009;49:1335–74.CrossRefGoogle Scholar
  19. 19.
    Manns MP, McHutchison JG, Gordon SC, et al. Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial. Lancet. 2001;358:958–65.CrossRefGoogle Scholar
  20. 20.
    Gupta V, Kumar A, Sharma P, et al. Sustained virological response rates to antiviral therapy in genotype 1 and 3 chronic hepatitis C patients: a study from north Inida. J Clin Exp Hepatol. 2014;4:287–92.CrossRefGoogle Scholar
  21. 21.
    Puri P, Anand AC, Saraswat VA, et al. Consensus statement of HCV task force of the Indian National Association for the Study of the Liver (INASL). Part I: status report of HCV infection in India. J Clin Exp Hepatol. 2014;4:106–16.CrossRefGoogle Scholar
  22. 22.
    Mir F, Kahveci AS, Ibdah JA, Tahan V. Sofosbuvir/velpatasvir regimen promises an effective pan-genotypic hepatitis C virus cure. Drug Des Devel Ther. 2017;11:497–502.CrossRefGoogle Scholar
  23. 23.
    Christdas J, Sivakumar J, David J, Daniel HD, Raghuraman S, Abraham P. Genotypes of hepatitis C virus in the Indian sub-continent: a decade long experience from a tertiary care hospital in South India. Indian J Med Microbiol. 2013;31:349–53.CrossRefGoogle Scholar
  24. 24.
    Chowdhury A, Santra A, Chaudhuri S, et al. Hepatitis C virus infection in the general population: a community-based study in West Bengal, India. Hepatology. 2003;37:802–9.CrossRefGoogle Scholar
  25. 25.
    Kumar JP, Puttamaregowda H. Distribution of hepatitis C virus genotypes in patients with chronic hepatitis C infection in Karnataka, South India. Int J Adv Med. 2016;3:905–7.CrossRefGoogle Scholar
  26. 26.
    Gretch D, Corey L, Wilson J, et al. Assessment of hepatitis C virus RNA levels by quantitative competitive RNA polymerase chain reaction: high titer viraemia correlates with advanced stage of disease. J Infect Dis. 1994;169:1219–25.CrossRefGoogle Scholar
  27. 27.
    Hagiwara H, Hayashi N, Mita E, et al. Quantitation of hepatitis C virus RNA in serum of asymptomatic blood donors and patients with type C chronic liver disease. Hepatology. 1993;17:545–50.CrossRefGoogle Scholar
  28. 28.
    Chakravarti A, Dogra G, Verma V, Srivastava AP. Distribution pattern of HCV genotypes and its association with viral load. Indian J Med Res. 2011;133:326–31.Google Scholar
  29. 29.
    Kanwal F, Kramer JR, Ilyas J, Duan Z, El-Sarang HB. HCV genotype 3 is associated with an increased risk of cirrhosis and hepatocellular cancer in a national sample of U. S Veterans with HCV. Hepatology. 2014;60:98–105.CrossRefGoogle Scholar
  30. 30.
    McMahon BJ, Bruden D, Townshend-Bulson L, et al. Infection with hepatitis C virus genotype 3 is an independent risk factor for end-stage liver disease, hepatocellular carcinoma and liver-related death. Clin Gastroenterol Hepatol. 2017;15:431–7.CrossRefGoogle Scholar

Copyright information

© Indian Society of Gastroenterology 2019

Authors and Affiliations

  • Balkumar Reddy Panyala
    • 1
  • Rathindra Mohan Mukherjee
    • 1
    Email author
  • Himaja Devarakonda
    • 1
  • Sivasathish Tadivaka
    • 1
  • Nagaraja Rao Padaki
    • 2
  • Mithun Sharma
    • 2
  • Nageshwar Reddy Duvvuru
    • 2
  1. 1.Asian Healthcare FoundationHyderabadIndia
  2. 2.Asian Institute of GastroenterologyHyderabadIndia

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