Advertisement

Indian Journal of Gastroenterology

, Volume 29, Issue 5, pp 201–204 | Cite as

Prevalence of extended spectrum beta lactamases in Salmonella species isolated from patients with acute gastroenteritis

  • Babu UmaEmail author
  • Kesani Prabhakar
  • Sadaiyappan Rajendran
  • Yelavarthi Lakshmi Sarayu
Short Report

Abstract

Introduction and aims

Extended-spectrum β-lactamases (ESBLs) continue to be a major problem world wide, conferring resistance to the expanded-spectrum cephalosporins. They are increasingly associated with genus Salmonella. This study aimed to determine the prevalence of ESBLs in Salmonella isolates in our region by phenotypic methods.

Methods

One thousand stool samples of acute gastroenteritis patients were screened and 42 strains of Salmonella (19 S. typhimurium, 14 S. enteritidis, 5 S. typhi, 3 S. paratyphi B 3 and 1 S. infantis) were detected.

Results

In the 3rd generation cephalosporin resistant Salmonella stains, ESBL was detected by double disc synergy test in ten isolates and by phenotypic confirmatory test in eight isolates of Salmonella. Drug resistance to various antibiotics was noted both among ESBL producers and non-ESBL producers. Resistance to chloramphenicol, nalidixic acid, norfloxacin and ciprofloxacin was noted in ESBL producers (n = 4, 3, 8 and 8 of eight cases respectively) and in non-ESBL producers (n = 10, 9, 16 and 13 of 34 cases, respectively). Quinolone resistance was significantly higher in ESBL producers while imipenem and cotrimoxazole resistance was significantly higher in non-ESBL producers. ESBL positive Salmonella isolates were found to be sensitive to amikacin and amoxyclav.

Conclusion

The proper and regular monitoring of drug resistance ESBL production among Salmonella strains were necessary for the effective therapeutic outcome.

Keywords

Beta lactamases Diarrhea Multidrug resistance 

Notes

Acknowledgement

The authors thank Dr. Kavitha, for kind help and support in collecting the clinical specimens and to the Central Research Institute, Kasauli, India for serotyping of the samples.

References

  1. 1.
    Bradford PA. Extended-spectrum beta-lactamases in the 21st century: characterization, epidemiology and detection of this important resistance threat. Clin Microbiol Rev. 2001;14:933–51.CrossRefPubMedGoogle Scholar
  2. 2.
    Kocagoz S, Budak F, Gur D. Evaluation of a chlorogenic medium for rapid detection of extended spectrum beta-lactamases producing Salmonella spp. Indian J Med Res. 2006;124:443–6.PubMedGoogle Scholar
  3. 3.
    Collee JG, Miles RS, Watt B. Tests for identification of bacteria. In: Collee JG, Fraser AG, Marmion BP, Simmons A, Eds. Mackie & McCartney Practical Medical Microbiology, 14th Ed, New York, Churchill Livingstone, 1996. pp. 131–49.Google Scholar
  4. 4.
    Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. CLSI approved standards M-100-S16 (M7). Wayne: Clinical and Laboratory Standards Institute; 2006.Google Scholar
  5. 5.
    Bauer AW, Kirby WMN, Sherries JL. Antibiotics susceptibility testing by standard disc method. Am J Clin Pathol. 1996;106:45–6.Google Scholar
  6. 6.
    Jarlier V, Nicolas MH, Fournier G, Philippon A. Extended broad-spectrum beta-lactamases conferring transferable resistance to newer beta-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns. Rev Infect Dis. 1988;10:867–78.PubMedGoogle Scholar
  7. 7.
    Rodriues C, Joshi P, Jani SH, Alphonse M, Radhakrishnan R, Mehta A. Detection of β-lactamases in nosocomial gram negative clinical isolates. Indian J Med Microbiol. 2004;22:247–50.Google Scholar
  8. 8.
    Taneja N, Mohan B, Khurana S, Sharma M. Antimicrobial resistance in selected bacterial enteropathogens in north India. Indian J Med Res. 2004;120:39–43.PubMedGoogle Scholar
  9. 9.
    Bhat P, Macaden R. Outbreaks of gastroenteritis due to multidrug resistant Salmonella typhimurium phage type 66/122/ T in Bangalore. Indian J Med Res. 1983;78:458–8.Google Scholar
  10. 10.
    Winokur PL, Canton R, Casellas JM, Legakis N. Variations in the prevalence of strains expressing an extended-spectrum β-lactamase phenotype and characterization of isolates from Europe, the Americas, and the Western Pacific region. Clin Infect Dis. 2001;32:94–103.CrossRefGoogle Scholar
  11. 11.
    Vahaboglu H, Fuzi S, Cetin S, et al. Characterization of extended beta-lactamase producing strains of Salmonella enterica serovar typhimurium with diverse resistance phenotypes. J Clin Microbiol. 2000;39:791–3.CrossRefGoogle Scholar
  12. 12.
    Mathur P, Kapil A, Das B, Dhawan B. Prevalence of extended spectrum beta lactamase producing gram negative bacteria in a tertiary care hospital. Indian J Med Res. 2002;115:153–7.PubMedGoogle Scholar
  13. 13.
    Katsanis GP, Spargo J, Ferraro MJ, Sutton L, Jacoby GA. Detection of Klebsiella pneumoniae and Escherichia coli strains producing extended-spectrum beta-lactamases. J Clin Microbiol. 1994;32:691–6.PubMedGoogle Scholar

Copyright information

© Indian Society of Gastroenterology 2010

Authors and Affiliations

  • Babu Uma
    • 1
    Email author
  • Kesani Prabhakar
    • 1
  • Sadaiyappan Rajendran
    • 1
  • Yelavarthi Lakshmi Sarayu
    • 1
  1. 1.Division of MicrobiologyRajah Muthiah Medical College and HospitalAnnamalainagarTamil NaduIndia

Personalised recommendations