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Is Mycobacterium avium subsp. paratuberculosis, the cause of Johne’s disease in animals, a good candidate for Crohn’s disease in man?

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Abstract

Mycobacterium avium subspecies paratuberculosis (MAP) causes Johne’s disease or paratuberculosis, a gastro intestinal inflammatory condition in ruminants and other animals, which is similar to Crohn’s disease (CD) that occurs in man. The role of MAP in the causation of CD has been under intense investigation in the last few decades. This review summarizes the status of MAP in animals and the food chain and its association with CD in man.

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References

  1. Collins MT. Diagnosis of paratuberculosis. Vet Clinics North Am Food Ani Prac 1996;12:357–371.

    CAS  Google Scholar 

  2. Chiodini RJ, Kruiningen HJ, Van Thayer WJ Jr, Coutu J. Spheroplastic phase of mycobacteria isolated from patients with Crohn’s disease. J Clin Microbiol 1986;24:357–363.

    CAS  PubMed  Google Scholar 

  3. Rhoades ER, Ullrich HJ. How to establish a lasting relationship with your host: Lessons learned from Mycobacterium spp. Immunol Cell Biol 2000;78:301–310.

    Article  CAS  PubMed  Google Scholar 

  4. Sohal JS, Singh SV, Singh PK, Singh AV. On the evolution of ‘Indian Bison type’ strains of Mycobacterium avium subsp paratuberculosis. Microbiol Res 2010;165:163–171.

    Article  CAS  PubMed  Google Scholar 

  5. Singh SV, Sohal JS, Singh PK, Singh AV. Genotype Profiles of Mycobacterium avium subspecies paratuberculosis isolates recovered from animals, commercial milk and human beings in North India. Int J infect Dis 2009;13:e221–e227.

    Article  CAS  PubMed  Google Scholar 

  6. Ayele YW, Machackova M, Pavlik I. The transmission and impact of paratuberculosis infection in domestic and wild ruminants. Veterinarni Medicina 2001;46:205–224.

    Google Scholar 

  7. Tiwari A, VanLeeuwen JA, McKenna SLB, Keefe GP, Barkema HW. Johne’s disease in Canada. Can Vet J 2006;47:874–882.

    PubMed  Google Scholar 

  8. Singh SV, Singh AV, Singh R, et al. Sero-prevalence of Johne’s disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis ‘Bison type’ genotype of goat origin. Comp Immunol Microbiol Infect Dis 2008;31:419–433.

    Article  CAS  PubMed  Google Scholar 

  9. Gao A, Mutharia L, Chen S, Rahn K, Odumeru J. Effect of pasteurization on survival of Mycobacterium paratuberculosis in milk. J Dairy Sci 2002;85:3198–3205.

    Article  CAS  PubMed  Google Scholar 

  10. Slana I, Paolicchi F, Janstova B, Navratilova P, Pavlik I. Detection methods for Mycobacterium avium subsp. paratuberculosis in milk and milk products: a review. Veterinarni Medicina 2008;6:283–306.

    Google Scholar 

  11. Shankar H, Singh SV, Singh PK, Singh AV, Sohal JS, Greenstein RJ. Presence characterization and genotype profiles of Mycobacterium avium subspecies paratuberculosis from unpasteurized individual and pooled milk, commercial pasteurized milk and milk products in India by culture, ELISA, PCR and PCR-REA methods. Int J Infect Dis 2009;14:e121–e126.

    Article  PubMed  Google Scholar 

  12. Manning EJB, Collins MT. Mycobacterium avium subsp. paratuberculosis: pathogenesis and diagnosis. Rev Sci Tech Off Int Epizoot 2001;20:133–150.

    CAS  Google Scholar 

  13. Mishina D, Katsel P, Brown ST, Gilberts EC, Greenstein RJ. On the etiology of Crohn’s disease. Proc Natl Acad Sci USA 1996;93:9816–9820.

    Article  CAS  PubMed  Google Scholar 

  14. Naser SA, Ghobrial G, Romero C, Valentine JF. Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease. Lancet 2004;364:1039–1044.

    Article  PubMed  Google Scholar 

  15. Naser SA, Schwartz D, Shafran I. Isolation of Mycobacterium avium subsp paratuberculosis from breast milk of Crohn’s disease patients. Am J Gastroenterol 2000;95:1094–1095.

    Article  CAS  PubMed  Google Scholar 

  16. Greenstein RJ. Is Crohn’s disease caused by a mycobacterium? Comparisons with leprosy, tuberculosis, and Johne’s disease. Lancet Infect Dis 2003;3:507–514.

    Article  PubMed  Google Scholar 

  17. Zur LS, Goethe R, Darji A, Valentin-Weigand P, Weiss S. Activation of macrophages and interference with CD4+ T-cell stimulation by Mycobacterium avium subspecies paratuberculosis and Mycobacterium avium subspecies avium. Immunology 2003;108:62–69.

    Article  Google Scholar 

  18. Clancy R, Ren Z, Turton J, Pang G, Wettstein A. Molecular evidence for Mycobacterium avium subspecies paratuberculosis (MAP) in Crohn’s disease correlates with enhanced TNF-α secretion. Dig Liver Dis 2007;39:445–451.

    Article  CAS  PubMed  Google Scholar 

  19. Geboes K, Collins S. Structural abnormalities of the nervous system in Crohn’s disease and ulcerative colitis. Neurogastroenterol Motil 2002;10:189–192.

    Article  Google Scholar 

  20. Scanu AM, Bull TJ, Cannas S, et al. Mycobacterium avium subspecies paratuberculosis infection in cases of irritable bowel syndrome and comparison with Crohn’s disease and Johne’s disease: common neural and immune pathogenicities. J Clin Microbiol 2007;45:3883–3890.

    Article  CAS  PubMed  Google Scholar 

  21. Singh PK, Singh SV, Singh AV, Sohal JS. Variability in susceptibility of different Indian goat breeds with respect to natural and experimental infection of Mycobacterium avium subspecies paratuberculosis. Indian J Small Rumin 2009;15:35–43.

    Google Scholar 

  22. Singh AV, Singh SV, Makharia GK, Singh PK, Sohal JS. Presence and characterization of Mycobacterium avium subspecies paratuberculosis from clinical and suspected cases of Crohn’s disease and in healthy human population in India. Int J Inf Dis 2008;12:190–197.

    Article  CAS  Google Scholar 

  23. Sechi LA, Scanu AM, Molicotti P, et al. Detection and isolation of Mycobacterium avium subspecies paratuberculosis from intestinal mucosal biopsies of patients with and without Crohn’s disease in Sardinia. Am J Gastroenterol 2005;100:1529–1536.

    Article  PubMed  Google Scholar 

  24. Bull TJ, McMinn EJ, Sidi-Boumedine K, et al. Detection and verification of Mycobacterium avium subspecies paratuberculosis in fresh ileocolonic mucosal biopsy specimens from individual with and without Crohn’s disease. J Clin Microbial 2003;41:2915–2923.

    Article  CAS  Google Scholar 

  25. Schwartz D, Shafran I, Romero C, et al. Use of short-term culture for identification of Mycobacterium avium subsp. paratuberculosis in tissue from Crohn’s disease patients. Clin Microbiol Infect 2000;6:303–307.

    Article  CAS  PubMed  Google Scholar 

  26. Collins MT, Lisby G, Moser C, et al. Results of multiple tests for Mycobacterium avium subspecies paratuberculosis in patients with inflammatory bowel disease and in control. J Clin Microbial 2000;38:4373–4381.

    CAS  Google Scholar 

  27. Clarkston WK, Presti ME, Petersen PF, et al. Role of Mycobacterium paratuberculosis in Crohn’s disease: a prospective, controlled study using polymerase chain reaction. Dis Colon Rectum 1998;41:195–199.

    Article  CAS  PubMed  Google Scholar 

  28. Kreuzpaintner G, Kirschner P, Wallnert A, et al. Mycobacteria of Runyon groups I, II and IV do not play an aetiological role in Crohn’s disease. Eur J Gastroenterol Hepatol 1995;7:1177–1182.

    Article  CAS  PubMed  Google Scholar 

  29. Ryan P, Bennett MW, Aarons S, et al. PCR detection of Mycobacterium paratuberculosis in Crohn’s disease granulomas isolated by laser capture micro-dissection. Gut 2002;51:665–670.

    Article  CAS  PubMed  Google Scholar 

  30. Fujita H, Eishi Y, Ishige I, et al. Quantitative analysis of bacterial DNA from Mycobacteria spp., Bacteroides vulgatus, and Escherichia coli in tissue samples from patients with inflammatory bowel diseases. J Gastroenterol 2002;37:509–516.

    Article  CAS  PubMed  Google Scholar 

  31. Cellier C, De Beenhouwer H, Berger A, et al. Mycobacterium paratuberculosis and Mycobacterium avium subsp. silvaticum DNA can not be detected by PCR in Crohn’s disease tissue. Gastroenterol Clin Biol 1998;22:675–678.

    CAS  PubMed  Google Scholar 

  32. Hulten K, El-Zimaity HM, Karttunen TJ, et al. Detection of Mycobacterium avium subspecies paratuberculosis in Crohn’s diseased tissues by in situ hybridization. Am J Gastroenterol 2001;96:1529–1535.

    Article  CAS  PubMed  Google Scholar 

  33. Butcher PD, McFadden JJ, Hermon-Taylor J. Investigation of mycobacteria in Crohn’s disease tissue by southern blotting and DNA hybridization with cloned mycobacterial genomic DNA probes from a Crohn’s disease isolated mycobacteria. Gut 1988;29:1222–1228.

    Article  CAS  PubMed  Google Scholar 

  34. Sechi LA, Mura M, Tanda F, et al. Identification of Mycobacterium avium subsp. paratuberculosis in biopsy specimens from patients with Crohn’s disease identified by in situ hybridization. J Clin Microbiol 2001;39:4514–4517.

    Article  CAS  PubMed  Google Scholar 

  35. Naser SA, Hulten K, Shafran I, Graham DY, El-Zaatari FAK. Specific seroreactivity of Crohn’s disease patients against p35 and p36 antigens of Mycobacterium avium subsp. paratuberculosis. Vet Microbiol 2000;77:497–504.

    Article  CAS  PubMed  Google Scholar 

  36. Nakase H, Nishio A, Tamaki H, et al. Specific antibodies against recombinant protein of insertion element 900 of Mycobacterium avium subspecies paratuberculosis in Japanese patients with Crohn’s disease. Inflamm Bowel Dis 2 2006;12:62–69.

    Article  Google Scholar 

  37. Thomas GA, Swift GL, Green J, et al. Controlled trial of anti-tuberculous chemotherapy in Crohn’s disease: a five year follow up study. Gut 1998;42:497–500.

    Article  CAS  PubMed  Google Scholar 

  38. Selby W, Pavli P, Crotty B, et al. Two-year combination antibiotic therapy with clarithromycin, rifabutin, and clofazimine for Crohn’s Disease. Gastroenterology 2007;132:2313–2319.

    Article  CAS  PubMed  Google Scholar 

  39. Greenstein RJ, Su L, Haroutunian V, Shahidi A, Brown ST. On the action of methotrexate and 6-mercaptopurine on M. avium subspecies paratuberculosis. PLoS ONE 2007;2:e161.

    Article  PubMed  Google Scholar 

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Singh, A.V., Singh, S.V., Singh, P.K. et al. Is Mycobacterium avium subsp. paratuberculosis, the cause of Johne’s disease in animals, a good candidate for Crohn’s disease in man?. Indian J Gastroenterol 29, 53–58 (2010). https://doi.org/10.1007/s12664-010-0019-y

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