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Journal of Maxillofacial and Oral Surgery

, Volume 19, Issue 1, pp 85–92 | Cite as

Is Impregnation of Xenograft with Caffeine Effective on Bone Healing Rate in Mandibular Defects? A Pilot Histological Animal Study

  • Sahand SamieiradEmail author
  • Vajiheh Mianbandi
  • Hamideh Salari Sedigh
  • Majid Hosseini-Abrishami
  • Farid Shiezadeh
  • Hossein Bagheri
  • Elahe Tohidi
  • Nasrollah Saghravanian
Original Article
  • 20 Downloads

Abstract

Introduction and Aim

The aim of this study was to evaluate the effect of two concentrations of caffeine (1.5% and 3%) powder added to Bio-Oss xenograft on bone healing rate of iatrogenic mandibular defects in dogs.

Materials and Method

The researchers implemented a pilot study on ten male adult mongrel dogs. Two 4-mm circular critical-sized defects were trephined on each side of the mandibular body (a total of 4 defects for each dog). One of the defects remained empty as a control group. The other three defects in each case were randomly filled with 1.5% or 3% caffeinated Bio-Oss or pure Bio-Oss. The mandible specimens were sent for histological and histomorphometric assessments, 4 months postoperatively. Our predictor variable was the type of bone substitute. The study outcomes were new bone formation, angiogenesis, and fibrosis. The p value was set at 0.05 using SPSS 16.

Results

The histological assessment showed that the administration of 1.5% caffeinated Bio-Oss to mandibular defects caused more angiogenesis and more new bone formation as well as less fibrosis compared to the other groups (p < 0.05).

Conclusion

This study suggested that the application of 1.5% caffeinated Bio-Oss in bone defects of dogs resulted in the higher new bone formation. However, further clinical trials are needed to support its relevancy.

Keywords

Caffeine Bio-Oss Xenograft New bone formation 

Notes

Acknowledgement

The authors would like to appreciate the continued support of the Research Council of Mashhad University of Medical Sciences. We also thank the cooperation of Dr. Taghi Ghiasi (general pathologist) and Dr. Amin Rahpeyma (oral and maxillofacial surgeon) in this research.

Funding

None.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Ethical Approval

All procedures performed in this animal study were in accordance with the ethical standards of our institute. In addition, all applicable international, national, and institutional guidelines for the care and use of animals were followed.

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Copyright information

© The Association of Oral and Maxillofacial Surgeons of India 2019

Authors and Affiliations

  • Sahand Samieirad
    • 1
    • 2
    Email author
  • Vajiheh Mianbandi
    • 2
  • Hamideh Salari Sedigh
    • 3
  • Majid Hosseini-Abrishami
    • 1
    • 2
  • Farid Shiezadeh
    • 1
  • Hossein Bagheri
    • 4
  • Elahe Tohidi
    • 5
  • Nasrollah Saghravanian
    • 1
  1. 1.Oral and Maxillofacial Diseases Research CenterMashhad University of Medical SciencesMashhadIran
  2. 2.Oral and Maxillofacial Surgery Department, Mashhad Dental SchoolMashhad University of Medical SciencesMashhadIran
  3. 3.Department of Clinical Sciences, Faculty of Veterinary MedicineFerdowsi University of MashhadMashhadIran
  4. 4.Dental Materials Research Center, Faculty of DentistryMashhad University of Medical SciencesMashhadIran
  5. 5.Oral and Maxillofacial RadiologistMashhadIran

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