Is Impregnation of Xenograft with Caffeine Effective on Bone Healing Rate in Mandibular Defects? A Pilot Histological Animal Study
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Introduction and Aim
The aim of this study was to evaluate the effect of two concentrations of caffeine (1.5% and 3%) powder added to Bio-Oss xenograft on bone healing rate of iatrogenic mandibular defects in dogs.
Materials and Method
The researchers implemented a pilot study on ten male adult mongrel dogs. Two 4-mm circular critical-sized defects were trephined on each side of the mandibular body (a total of 4 defects for each dog). One of the defects remained empty as a control group. The other three defects in each case were randomly filled with 1.5% or 3% caffeinated Bio-Oss or pure Bio-Oss. The mandible specimens were sent for histological and histomorphometric assessments, 4 months postoperatively. Our predictor variable was the type of bone substitute. The study outcomes were new bone formation, angiogenesis, and fibrosis. The p value was set at 0.05 using SPSS 16.
The histological assessment showed that the administration of 1.5% caffeinated Bio-Oss to mandibular defects caused more angiogenesis and more new bone formation as well as less fibrosis compared to the other groups (p < 0.05).
This study suggested that the application of 1.5% caffeinated Bio-Oss in bone defects of dogs resulted in the higher new bone formation. However, further clinical trials are needed to support its relevancy.
KeywordsCaffeine Bio-Oss Xenograft New bone formation
The authors would like to appreciate the continued support of the Research Council of Mashhad University of Medical Sciences. We also thank the cooperation of Dr. Taghi Ghiasi (general pathologist) and Dr. Amin Rahpeyma (oral and maxillofacial surgeon) in this research.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflicts of interest.
All procedures performed in this animal study were in accordance with the ethical standards of our institute. In addition, all applicable international, national, and institutional guidelines for the care and use of animals were followed.
- 1.Khojasteh A, Fahimipour F, Jafarian M, Sharifi D, Jahangir S, Khayyatan F et al (2017) Bone engineering in dog mandible: coculturing mesenchymal stem cells with endothelial progenitor cells in a composite scaffold containing vascular endothelial growth factor. J Biomed Mater Res B Appl Biomater 105(7):1767–1777CrossRefGoogle Scholar
- 2.Jafarian M, Eslaminejad MB, Khojasteh A, Abbas FM, Dehghan MM, Hassanizadeh R et al (2008) Marrow-derived mesenchymal stem cells-directed bone regeneration in the dog mandible: a comparison between biphasic calcium phosphate and natural bone mineral. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105(5):e14–e24CrossRefGoogle Scholar
- 3.Khojasteh A, Dashti SG, Dehghan MM, Behnia H, Abbasnia P, Morad G (2014) The osteoregenerative effects of platelet-derived growth factor BB cotransplanted with mesenchymal stem cells, loaded on freeze-dried mineral bone block: a pilot study in dog mandible. J Biomed Mater Res B Appl Biomater 102(8):1771–1778CrossRefGoogle Scholar
- 5.Singh J, Takhar RK, Bhatia A, Goel A (2016) Bone graft materials: dental aspects. J Nov Res Healthc Nurs 3:99–103Google Scholar
- 15.Lucaciu O, Gheban D, Soriţau O, Băciuţ M, Câmpian RS, Băciuţ G (2015) Comparative assessment of bone regeneration by histometry and a histological scoring system/Evaluarea comparativă a regenerării osoase utilizând histometria şi un scor de vindecare histologică. Romanian Rev Lab Med 23(1):31–45Google Scholar
- 25.Bonardi JP, dos Santos Pereira R, Lima FB, Faverani LP, Griza GL, Okamoto R et al (2018) Prospective and randomized evaluation of ChronOS and Bio-Oss in human maxillary sinuses: histomorphometric and immunohistochemical assignment for Runx 2, vascular endothelial growth factor, and osteocalcin. J Oral Maxillofac Surg 76(2):325–335CrossRefGoogle Scholar