Journal of Maxillofacial and Oral Surgery

, Volume 14, Issue 4, pp 907–913 | Cite as

Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study

  • Soh Nishimoto
  • Kazutoshi Fujita
  • Yohei Sotsuka
  • Masato Kinoshita
  • Toshihiro Fujiwara
  • Kenichiro Kawai
  • Masao Kakibuchi
Research Paper



The aim of this study was to compare growth factor amount contained in platelet rich fibrin (PRF) and compare with that in platelet rich plasma (PRP), and in whole blood. And also to investigate distribution of growth factors and cellular components in PRF.

Materials and Methods

PRF and PRP were obtained from the same sample of peripheral blood. Extraction of proteins were done with lysis buffer, accompanied by freeze and thaw procedures. Concentration of two representative growth factors in platelets: platelet derived growth factor (PDGF) and transforming growth factor beta (TGF-β), were measured with enzyme-linked immunosorbent assay (ELISA). PRF was cut into three parts: (top, middle and bottom), and growth factor concentration was measured respectively. Paraffin embedded section of PRF was observed with Giemsa stain. Immuno-histochemical analysis with anti-PDGF and anti-TGF-β antibodies was also conducted.


The growth factor levels in PRF was higher than in peripheral blood and comparable to those in PRP. Growth factor levels in bottom part of PRF was much higher than in top and middle part. Microscopically, platelets and mono-nucleated cells were concentrated just above the yellow–red interface. Poly-nucleated cells were concentrated below the interface.


The growth factors were surely concentrated in PRF. This result can support basis of good clinical outcomes. For effective application of PRF, the knowledge that growth factors and cells are not equally distributed in PRF should be utilized.


Platelet rich fibrin Growth factor Mono-nucleated cells ELISA Immuno-histochemistry 



No funding was received for the preparation of this article. The authors have no financial interest in any of the products or devices mentioned in the article.


  1. 1.
    Ross R (1979) Platelets: cell proliferation and atherosclerosis. Metabolism 28:410–414CrossRefPubMedGoogle Scholar
  2. 2.
    Assoian RK, Komoriya A, Meyers CA et al (1983) Transforming growth factor-beta in human platelets. Identification of a major storage site, purification, and characterization. J Biol Chem 258:7155–7160PubMedGoogle Scholar
  3. 3.
    Marx RE, Carlson ER, Eichstaedt RM et al (1998) Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 85:638–646CrossRefPubMedGoogle Scholar
  4. 4.
    Eppley BL, Woodell JE, Higgins J (2004) Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing. Plast Reconstr Surg 114:1502–1508CrossRefPubMedGoogle Scholar
  5. 5.
    Wiltfang J, Kloss FR, Kessler P et al (2004) Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. An animal experiment. Clin Oral Implants Res 15:187–193CrossRefPubMedGoogle Scholar
  6. 6.
    Crovetti G, Martinelli G, Issi M et al (2004) Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 30:145–151. doi: 10.1016/j.transci.2004.01.004 CrossRefPubMedGoogle Scholar
  7. 7.
    Oyama T, Nishimoto S, Tsugawa T, Shimizu F (2004) Efficacy of platelet-rich plasma in alveolar bone grafting. J Oral Maxillofac Surg 62:555–558CrossRefPubMedGoogle Scholar
  8. 8.
    Choukroun J, Adda F, Schoeffler CVA (2001) Une opportunité en paro-implantologie: le PRF. Implantodontie 42:55–62Google Scholar
  9. 9.
    Dohan DM, Choukroun J, Diss A et al (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e37–e44. doi: 10.1016/j.tripleo.2005.07.008 CrossRefPubMedGoogle Scholar
  10. 10.
    Saluja H, Dehane V, Mahindra U (2011) Platelet-Rich fibrin: a second generation platelet concentrate and a new friend of oral and maxillofacial surgeons. Ann Maxillofac Surg 1:53–57. doi: 10.4103/2231-0746.83158 PubMedCentralCrossRefPubMedGoogle Scholar
  11. 11.
    Sharma A, Pradeep AR (2011) Treatment of 3-wall intrabony defects in patients with chronic periodontitis with autologous platelet-rich fibrin: a randomized controlled clinical trial. J Periodontol 82:1705–1712. doi: 10.1902/jop.2011.110075 CrossRefPubMedGoogle Scholar
  12. 12.
    Ruga E, Gallesio C, Boffano P (2011) Platelet-rich fibrin and piezoelectric surgery: a safe technique for the prevention of periodontal complications in third molar surgery. J Craniofac Surg 22:1951–1955. doi: 10.1097/SCS.0b013e31822ea76b CrossRefPubMedGoogle Scholar
  13. 13.
    Chignon-Sicard B, Georgiou CA, Fontas E et al (2012) Efficacy of leukocyte- and platelet-rich fibrin in wound healing: a randomized controlled clinical trial. Plast Reconstr Surg 130:819e–829e. doi: 10.1097/PRS.0b013e31826d1711 CrossRefPubMedGoogle Scholar
  14. 14.
    Singh A, Kohli M, Gupta N (2012) Platelet rich fibrin: a novel approach for osseous regeneration. J Maxillofac Oral Surg 11:430–434. doi: 10.1007/s12663-012-0351-0 PubMedCentralCrossRefPubMedGoogle Scholar
  15. 15.
    Girish Rao S, Bhat P, Nagesh KS et al (2013) Bone regeneration in extraction sockets with autologous platelet rich fibrin gel. J Maxillofac Oral Surg 12:11–16. doi: 10.1007/s12663-012-0370-x PubMedCentralCrossRefPubMedGoogle Scholar
  16. 16.
    Dohan DM, Choukroun J, Diss A et al (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101:e45–e50. doi: 10.1016/j.tripleo.2005.07.009 CrossRefPubMedGoogle Scholar
  17. 17.
    Nishimoto S, Oyama T, Matsuda K (2007) Simultaneous concentration of platelets and marrow cells: a simple and useful technique to obtain source cells and growth factors for regenerative medicine. Wound Repair Regen 15:156–162. doi: 10.1111/j.1524-475X.2006.00196.x CrossRefPubMedGoogle Scholar
  18. 18.
    Pluemsakunthai W, Kuroda S (2013) A basic analysis of platelet-rich fibrin: distribution and release of platelet-derived growth factor-BB. Inflammation and Regeneration. doi:  10.2492/inflammregen.33.164
  19. 19.
    Margolis J (1957) Initiation of blood coagulation by glass and related surfaces. J Physiol 137:95–109PubMedCentralCrossRefPubMedGoogle Scholar
  20. 20.
    Ratnoff OD, Rosenblum JM (1958) Role of Hageman factor in the initiation of clotting by glass; evidence that glass frees Hageman factor from inhibition. Am J Med 25:160–168CrossRefPubMedGoogle Scholar
  21. 21.
    Wilner GD, Nossel HL, LeRoy EC (1968) Activation of Hageman factor by collagen. J Clin Invest 47:2608–2615. doi: 10.1172/JCI105943 PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© The Association of Oral and Maxillofacial Surgeons of India 2015

Authors and Affiliations

  • Soh Nishimoto
    • 1
  • Kazutoshi Fujita
    • 1
  • Yohei Sotsuka
    • 1
  • Masato Kinoshita
    • 1
  • Toshihiro Fujiwara
    • 1
  • Kenichiro Kawai
    • 1
  • Masao Kakibuchi
    • 1
  1. 1.Department of Plastic SurgeryHyogo College of MedicineNishinomiyaJapan

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