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Journal of Maxillofacial and Oral Surgery

, Volume 14, Issue 4, pp 907–913 | Cite as

Growth Factor Measurement and Histological Analysis in Platelet Rich Fibrin: A Pilot Study

  • Soh Nishimoto
  • Kazutoshi Fujita
  • Yohei Sotsuka
  • Masato Kinoshita
  • Toshihiro Fujiwara
  • Kenichiro Kawai
  • Masao Kakibuchi
Research Paper

Abstract

Objective

The aim of this study was to compare growth factor amount contained in platelet rich fibrin (PRF) and compare with that in platelet rich plasma (PRP), and in whole blood. And also to investigate distribution of growth factors and cellular components in PRF.

Materials and Methods

PRF and PRP were obtained from the same sample of peripheral blood. Extraction of proteins were done with lysis buffer, accompanied by freeze and thaw procedures. Concentration of two representative growth factors in platelets: platelet derived growth factor (PDGF) and transforming growth factor beta (TGF-β), were measured with enzyme-linked immunosorbent assay (ELISA). PRF was cut into three parts: (top, middle and bottom), and growth factor concentration was measured respectively. Paraffin embedded section of PRF was observed with Giemsa stain. Immuno-histochemical analysis with anti-PDGF and anti-TGF-β antibodies was also conducted.

Results

The growth factor levels in PRF was higher than in peripheral blood and comparable to those in PRP. Growth factor levels in bottom part of PRF was much higher than in top and middle part. Microscopically, platelets and mono-nucleated cells were concentrated just above the yellow–red interface. Poly-nucleated cells were concentrated below the interface.

Conclusion

The growth factors were surely concentrated in PRF. This result can support basis of good clinical outcomes. For effective application of PRF, the knowledge that growth factors and cells are not equally distributed in PRF should be utilized.

Keywords

Platelet rich fibrin Growth factor Mono-nucleated cells ELISA Immuno-histochemistry 

Notes

Acknowledgments

No funding was received for the preparation of this article. The authors have no financial interest in any of the products or devices mentioned in the article.

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Copyright information

© The Association of Oral and Maxillofacial Surgeons of India 2015

Authors and Affiliations

  • Soh Nishimoto
    • 1
  • Kazutoshi Fujita
    • 1
  • Yohei Sotsuka
    • 1
  • Masato Kinoshita
    • 1
  • Toshihiro Fujiwara
    • 1
  • Kenichiro Kawai
    • 1
  • Masao Kakibuchi
    • 1
  1. 1.Department of Plastic SurgeryHyogo College of MedicineNishinomiyaJapan

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