Journal of Maxillofacial and Oral Surgery

, Volume 13, Issue 4, pp 464–470 | Cite as

Comparative, Histological and Histomorphometric Analysis of Three Anorganic Bovine Xenogenous Bone Substitutes: Bio-Oss, Bone-Fill and Gen-Ox Anorganic

  • Rafael ManfroEmail author
  • Fabiano Silva Fonseca
  • Marcelo Carlos Bortoluzzi
  • Wilson Roberto Sendyk
Research Paper



Anorganic bovine xenogenous grafts show the best performance as bone substitutes in implantodontics. Bio-Oss is the world’s most widely used and investigated anorganic bone substitute. This article compares two anorganic bovine bone substitutes (Bone-Fill and Gen-Ox anorganic) with Bio-Oss.

Materials and Methods

Eight New Zealand rabbits were implanted with 4 titanium cylinders randomly filled with Bio-Oss, Bone-Fill, Gen-Ox anorganic or a blood clot. Four animals were sacrificed after 8 weeks; 12 weeks later, the remaining four were sacrificed. The contents of the cylinders were removed, cut and stained with HE before they were evaluated with an optical microscope. The samples were submitted to histomorphometry for analysis.


The bone formation with Bio-Oss at 8 weeks was 8.43 mm2; at 12 weeks, it was 9.32 mm2. The bone formation with Bone-Fill at 8 weeks was 7.24 mm2; at 12 weeks, it was 9.01 mm2. The bone formation with Gen-Ox anorganic at 8 weeks was 2.78 mm2; at 12 weeks, it was 3.02 mm2. The bone formation with the blood clot at 8 weeks was 0.65 mm2; at 12 weeks, it was 0.63 mm2.


Following this model, Bone-Fill was comparable to Bio-Oss and superior to Gen-Ox and blood clot.


Osseointegrated implants Bone reconstruction Anorganic bovine xenogenous graft 



The authors are grateful to Sistema de Implantes Nacional represented by Ms Neide Lenharo and Dr. Ariel Lenharo for donating the titanium cylinders and screws used to conduct the experiments. The authors also thank Dr. Nelson Villa (Department of Histology, School of Dentistry, University of Santo Amaro/SP) and Dr. Andre Pelegrine for surgical assistance. The authors report no conflicts of interest related to this study.


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Copyright information

© Association of Oral and Maxillofacial Surgeons of India 2013

Authors and Affiliations

  • Rafael Manfro
    • 1
    Email author
  • Fabiano Silva Fonseca
    • 2
  • Marcelo Carlos Bortoluzzi
    • 3
  • Wilson Roberto Sendyk
    • 4
  1. 1.Department of Dental ImplantologySOEBRASFlorianópolisBrazil
  2. 2.VitóriaBrazil
  3. 3.Department of Implantodontic Maxillofacial SurgeryUNOESCJoaçabaBrazil
  4. 4.UNISASanto AmaroBrazil

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