Comparative, Histological and Histomorphometric Analysis of Three Anorganic Bovine Xenogenous Bone Substitutes: Bio-Oss, Bone-Fill and Gen-Ox Anorganic
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Anorganic bovine xenogenous grafts show the best performance as bone substitutes in implantodontics. Bio-Oss is the world’s most widely used and investigated anorganic bone substitute. This article compares two anorganic bovine bone substitutes (Bone-Fill and Gen-Ox anorganic) with Bio-Oss.
Materials and Methods
Eight New Zealand rabbits were implanted with 4 titanium cylinders randomly filled with Bio-Oss, Bone-Fill, Gen-Ox anorganic or a blood clot. Four animals were sacrificed after 8 weeks; 12 weeks later, the remaining four were sacrificed. The contents of the cylinders were removed, cut and stained with HE before they were evaluated with an optical microscope. The samples were submitted to histomorphometry for analysis.
The bone formation with Bio-Oss at 8 weeks was 8.43 mm2; at 12 weeks, it was 9.32 mm2. The bone formation with Bone-Fill at 8 weeks was 7.24 mm2; at 12 weeks, it was 9.01 mm2. The bone formation with Gen-Ox anorganic at 8 weeks was 2.78 mm2; at 12 weeks, it was 3.02 mm2. The bone formation with the blood clot at 8 weeks was 0.65 mm2; at 12 weeks, it was 0.63 mm2.
Following this model, Bone-Fill was comparable to Bio-Oss and superior to Gen-Ox and blood clot.
KeywordsOsseointegrated implants Bone reconstruction Anorganic bovine xenogenous graft
The authors are grateful to Sistema de Implantes Nacional represented by Ms Neide Lenharo and Dr. Ariel Lenharo for donating the titanium cylinders and screws used to conduct the experiments. The authors also thank Dr. Nelson Villa (Department of Histology, School of Dentistry, University of Santo Amaro/SP) and Dr. Andre Pelegrine for surgical assistance. The authors report no conflicts of interest related to this study.
- 7.Slott EC, Lundgren D, Burgos PM (2003) Placement of autogeneic bone chips or bovine bone mineral in guided bone augmentation: a rabbit skull study. Int J Oral Maxillofac Implants 18:795–805Google Scholar
- 9.Urban IA, Nagursky H, Lozada JL (2011) Horizontal ridge augmentation with a resorbable membrane and particulated autogenous bone with or without anorganic bovine bone-derived mineral: a prospective case series in 22 patients. Int J Oral Implants 26:404–414Google Scholar
- 16.Palmieri A, Pezzetti F, Brunelli G, Martinelli M, Muzio L, Scarano A, Scapoli L, Arlotti M, Guerzoni L, Carinci F (2010) Anorganic bovine bone (Bio-Oss) regulates mi RNA of osteoblast-like cells. Int J Periodontics Restor Dent 30:83–87Google Scholar
- 17.Piatelli M, Favero GA, Scarano A, Orsini G, Piatelli A (1999) Bone reactions to anorganic bovine bone (Bio-Oss) used in sinus augmentation procedures: a histologic long-term report of 20 cases in humans. Int J. Oral Maxillofac Implants 14:835–840Google Scholar
- 21.Ezirganlı S, Polat S, Barıs E, Tatar I, Celik HH (2013) Comparative investigation of the effects of different materials used with a titanium barrier on new bone formation. Clin Oral Impl Res 24(312):319Google Scholar
- 27.Frost HM (1963) Bone remodeling dynamics. Charles C Thomas Publisher, SpringfieldGoogle Scholar