Effect of Phenolic Acids Derived from Rice Straw on Botrytis cinerea and Infection on Tomato

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Phenolic compounds are widely used in different research fields, such as pesticides, medicines, and food. In this study, phenolic acids (PAs) were extracted from rice straw and were found to exhibit a strong inhibitory effect on Botrytis cinerea. B. cinerea mycelial growth and spore generation decreased by 86.18% and 69.10%, respectively, following 0.2 g/L phenolic acid treatment. Confocal microscopic images demonstrated that phenolic acids changed the morphology of B. cinerea. The addition of phenolic acids to B. cinerea-infected tomato leaves increased PAL (phenylalaninammo-nialyase) and PPO (polyphenol oxidase) activities, and decreased POD (peroxidases) and CAT (catalase) activities in the leaves, indicating that phenolic acids enhanced the tolerance of tomato leaves to B. cinerea by reducing oxidative stress. Chlorophyll fluorescence imaging revealed that phenolic acids could alleviate the destruction of the photosynthetic system of B. cinerea-infected leaves. These results provide new insight into the use of phenolic acids from rice straw, through which a complete green cycle of ecological production can be established.

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  1. 1.

    Yu, W., Zhao, R., Sheng, J., Shen, L.: SlERF2 is associated with methyl jasmonate-mdeiated defense response against Botrytis cinerea in tomato fruit. J. Agric. Food Chem. 66(38), 9923–9932 (2018)

  2. 2.

    Prins, T.W., Tudzynski, P., von Tiedemann, A., Tudzynski, B., Ten Have, A., Hansen, M.E., Van Kan, J.A.L.: Infection strategies of Botrytis cinerea and related necrotrophic pathogens. Fungal Pathology, pp. 33–64. Springer, Netherlands (2000)

  3. 3.

    Fillinger, S., Elad, Y.: A plant hosts of Botrytis spp. Botrytis—the fungus, the pathogen and its management in agricultural systems. pp. 413–486. Springer International Publishing, Cham (2016)

  4. 4.

    Cristescu, S.M., De Martinis, D., te Lintel Hekkert, S., Parker, D.H., Harren, F.J.M.: Ethyleneproduction by Botrytis cinerea in vitro and in tomatoes. Appl. Environ. Microbiol. 68(11), 5342–5350 (2002)

  5. 5.

    Zhang, Y., Yang, X., Liu, Q., Qiu, D., Zhang, Y., Zeng, H., Yuan, J., Mao, J.: Purification of novel protein elicitor from Botrytis cinerea that induces disease resistance and drought tolerance in plants. Microbiol. Res. 165(2), 142–151 (2010)

  6. 6.

    Puangpronpitag, D., Sittiwet, C.: Antimicrobial properties of cinnamomum verum aqueous extracts. Asian J. Biol. Sci. 2(2), 49–53 (2009)

  7. 7.

    Agatemor, C.: Antimicrobial activity of aqueous and ethanol extracts of nine Nigerian spices against four food borne bacteria. Electron. J. Environ. Agric. Food Chem. 10(3), 77–80 (2009)

  8. 8.

    de Rodríguez, D.J., Hernández-Castillo, D., Angulo-Sánchez, J.L., et al.: Antifungal activity in vitro of Flourensia spp. extracts on Alternaria sp. Rhizoctonia solani, and Fusarium oxysporum. Ind. Crops Prod. 25(2), 111–116 (2007)

  9. 9.

    Flores-Moctezuma, H., García Licona, R., Sandoval García, G., Zilch Domínguez, S., Bermúdez Torres, K., Bravo-Luna, L., Martínez Martínez, G., Carvajal-Moreno, M., Montes Belmont, R., Cruz Cruz, V.: Antifungal properties in higher plants. Retrospective analyses and investigations. Rev. Mex. de Fitopatología 18, 125–131 (2000)

  10. 10.

    Mendez, M., Rodríguez, R., Ruiz, J., Morales-Adame, D., Castillo, F., Hernández-Castillo, F.D., Aguilar, C.N.: Antibacterial activity of plant extracts obtained with alternative organics solvents against food-borne pathogen bacteria. Ind. Crops Prod. 37(1), 445–450 (2012)

  11. 11.

    Wang, L., Hu, W., Deng, J., Liu, X., Zhou, J., Li, X.: Antibacterial activity of Litsea cubeba essential oil and its mechanism against Botrytis cinerea. RSC Adv. 9, 28987–28995 (2019)

  12. 12.

    Axelsson, L., Franzn, M., Ostwald, M., Berndes, G., Lakshmi, G., Ravindranath, N.H.: Perspective: Jatropha cultivation in Southern India: assessing farmers’ experiences. Biofuels Bioprod. Biorefin. 6(3), 246–256 (2012)

  13. 13.

    Lee, K.M., Kalyani, D., Tiwari, M.K., Kim, T.S., Dhiman, S.S., Lee, J.K., Kim, I.W.: Enhanced enzymatic hydrolysis of rice straw by removal of phenolic compounds using a novel laccase from yeast Yarrowia lipolytica. Bioresour. Technol. 123(4), 636–645 (2012)

  14. 14.

    Yilmaz, V.A., Brandolini, A., Hidalgo, A.: Phenolic acids and antioxidant activity of wild, feral and domesticated diploid wheats. J. Cereal Sci. 64, 168–175 (2015)

  15. 15.

    Black, R.L.B., Dix, N.J.: Spore germination and germ hyphal growth of microfungi from litter and soil in the presence of ferulic acid. Trans. Br. Mycol. Soc. 66(2), 305–311 (1976)

  16. 16.

    Ohi, M., Kitamura, T., Hata, S.: Stimulation by caffeic acid, coumalic acid, and corilagin of the germination of resting spores of the clubroot pathogen Plasmodiophora brassicae. J. Agric. Chem. Soc. Jpn. 67(1), 170–173 (2014)

  17. 17.

    Xue, Y., Wang, X., Chen, X., Hu, J., Gao, M.-T., Li, J.: Effects of different cellulases on the release of phenolic acids from rice straw during saccharification. Bioresour. Technol. 234, 208–216 (2017)

  18. 18.

    Zheng, W., Zheng, Q., Xue, Y., Hu, J., Gao, M.-T.: Influence of rice straw polyphenols on cellulase production by Trichoderma reesei. J. Biosci. Bioeng. 123(6), 731–738 (2017)

  19. 19.

    Wang, X., Tsang, Y.F., Li, Y., Ma, X., Cui, S., Zhang, T.-A., Hu, J., Gao, M.-T.: Inhibitory effects of phenolic compounds of rice straw formed by saccharification during ethanol fermentation by Pichia stipitis. Bioresour. Technol. 244, 1059–1067 (2017)

  20. 20.

    Chen, X., Xue, Y., Hu, J., Tsang, Y.F., Gao, M.-T.: Release of polyphenols is the major factor influencing the bioconversion of rice straw to lactic acid. Appl. Biochem. Biotechnol. 183(3), 685–698 (2017)

  21. 21.

    Zheng, W., Chen, X., Xue, Y., Hu, J., Gao, M.-T., Tsang, Y.F.: The influence of soluble polysaccharides derived from rice straw upon cellulase production by Trichoderma reesei. Process Biochem. 61, 130–136 (2017)

  22. 22.

    Chen, X., Wang, X., Xue, Y., Zhang, T.-A., Hu, J., Tsang, Y.F., Gao, M.-T.: Tapping the bioactivity potential of residual stream from its pretreatments may be a green strategy for low-cost bioconversion of rice straw. Appl. Biochem. Biotechnol. 186(3), 507–524 (2018)

  23. 23.

    Chen, Q., Li, T., Gui, M., Liu, S., Zheng, M., Ni, J.: Effects of ZnO nanoparticles on aerobic denitrification by strain Pseudomonas stutzeri PCN-1. Bioresour. Technol. 239, 21–27 (2017)

  24. 24.

    Zhang, X., Hao, L., Hong, K., Yi, Y.: Growth, dendrobine content and photosynthetic characteristics of Dendrobium nobile under different solar irradiances. Plant Omics 7(6), 461–467 (2014)

  25. 25.

    Chen, X., Wang, X., Xue, Y., Zhang, T.A., Li, Y., Hu, J., Tsang, Y.F., Zhang, H., Gao, M.T.: Influence of rice straw-derived dissolved organic matter on lactic acid fermentation by Rhizopus oryzae. J. Biosci. Bioeng. 125(6), 703–709 (2018)

  26. 26.

    Perrin D.D., Watt, A.E.: Complex formation of zinc and cadmium with glutathione. BBA 230(1), 96–104 (1971)

  27. 27.

    Singhal, G.M, Das, N.B., Sharma, R.P.: ChemInform abstract: reaction of nitroalkenes with iodotrimethylsilane: a new method for the conversion of vinyl nitro steroids to keto steroids. Chem. Inform. 21(14), 1470–1471 (1990)

  28. 28.

    Morales, J., Mendoza, L., Cotoras, M.: Alteration of oxidative phosphorylation as a possible mechanism of the antifungal action of p-coumaric acid against Botrytis cinerea. J. Appl. Microbiol. 123(4), 969–976 (2017)

  29. 29.

    Geny, L., Darrieumerlou, A., Doneche, B.: Conjugated polyamines and hydroxycinnamic acids in grape berries during Botrytis cinerea disease development differences between ‘noble rot’ and ‘grey mould’. Aust. J. Grape Wine Res. 9(2), 102–106 (2003)

  30. 30.

    Dandan, X., Yizhen, D., Pinggen, X., Qi, W., Zide, J., Lingwang, G.: In vitro and in vivo effectiveness of phenolic compounds for the control of postharvest gray mold of table grapes. Postharvest Biol. Technol. 139, 106–114 (2018)

  31. 31.

    Polle, A., Otter, T., Seifert, F.: Apoplastic peroxidases and lignification in needles of Norway Spruce (Picea abies 1). Plant Cell Physiol. 106(1), 53–60 (1994)

  32. 32.

    AbuQamar, S., Moustafa, K., Tran, L.S.: Mechanisms and strategies of plant defense against Botrytis cinerea. Crit. Rev. Biotechnol. 37(2), 262–274 (2017)

  33. 33.

    Zhao, P., Ren, A., Dong, P., Sheng, Y., Chang, X., Zhang, X.: The antimicrobial peptaibol trichokonin IV promotes plant growth and induces systemic resistance against Botrytis cinerea infection in moth orchid. J. Phytopathol. 166(5), 346–354 (2018)

  34. 34.

    van den Winkel, D., Bastiaans, H.M.M., Bickelhaupt, F.: Phosphasilene synthesis and reactivity: an improved route to 1-(2,4,6-tri-tert-butylphenyl)-2-tert-butyl-2-(2,4,6-tri-isopropylphenyl)phosphasilene. J. Organomet. Chem. 405(2), 183–194 (1991)

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This work was supported by the Special Fund for Agroscientific Research in the Public Interest (No. 201503135-14); Scientific Research Projects of Shanghai Science and Technology Committee (16391902000).

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Correspondence to Min-Tian Gao.

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Hou, R., Shi, J., Ma, X. et al. Effect of Phenolic Acids Derived from Rice Straw on Botrytis cinerea and Infection on Tomato. Waste Biomass Valor (2020).

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  • Agricultural waste
  • Bioactives
  • Phenolic compound
  • Antimicrobial activity
  • Plant growth
  • Fungicide