Evaluation of Various Lipid Extraction Techniques for Microalgae and Their Effect on Biochemical Components

  • Reena Singh
  • Ashutosh Kumar
  • Yogesh Chandra SharmaEmail author
Original Paper


Spirulina is a potential feedstock for biofuel production and rich source of other valuable products such as lipid, reduced sugar and protein content. The aim of this study is to achieve the maximum lipid yield and other value added products from lipid extracted Spirulina by using different techniques. The parameters, pre-treatment of ultra sonication, drying techniques and methods of cell disruption techniques were studied with respect to the biochemical properties. The cell disruption techniques involving temperature and pressure for disruption opted were autoclave, microwave and conventional heating processes. The techniques were analysed for considerable amount of lipid yield. The interaction between drying techniques and pre-treatment with cell disruption techniques were also investigated by two way ANOVA. The effect on morphology of disrupted cell was observed by scanning electron microscopy characterization. The study revealed that the oven dried biomass resulted in better yield of protein and lipid as compared to sun dried biomass. In case of reduced sugar, the fresh algal cell and microwave assisted cell biomass were found to give better yield for sun dried biomass. The experimental data revealed that among used methods for lipid extraction, the microwave and conventional heating methods were found to be the best out of the techniques used for lipid extraction and reduced sugar yield.


Spirulina Drying technique Solvothermal Lipid extraction Biochemical analysis 



The authors are thankful to Indian Institute of Technology (BHU) and Department of Science and Technology for funding the project under DST-IBSA trilateral programme. Authors also thank the Indian Biodiesel Corporation Baramati, Pune, Maharastra for providing the microalgae culture of S. platensis.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Reena Singh
    • 1
  • Ashutosh Kumar
    • 1
  • Yogesh Chandra Sharma
    • 1
    Email author
  1. 1.Department of ChemistryIndian Institute of Technology (BHU) VaranasiVaranasiIndia

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