Bacterial Succession in the Thermophilic Phase of Composting of Anaerobic Digestates
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Organic matter degradation and bacterial communities associated to the thermophilic phase of composting were compared using two different types of anaerobic digestates, one from a sewage sludge digester (SD), and the other from an agricultural digester (AD). The composting process exhibited similar variations in temperature, pH, moisture content and bacterial profiles, despite the inherent feedstock differences along with distinctive initial bacterial composition. According to the data obtained from 16S rRNA gene amplicon sequencing, SD constituted more than 20 bacterial phyla with Proteobacteria (21%) and Chloroflexi (21%) being predominant, meanwhile AD was represented by only 7 phyla in which Firmicutes was the most abundant phylum (73%). Nevertheless, bacterial community profiles of the two composting systems became more similarly represented at the phylum level, both dominated by Proteobacteria (65% in AD and 61% in SD), whereas Chromatiaceae and Sphingomonadaceae were the most abundant families in AD and SD, respectively. Highly diverse but similar bacterial communities were detected during the composting of different anaerobic digestates at the thermophilic phase.
KeywordsAgricultural waste Anaerobic digestate Composting Microbial community Sewage sludge
This study was supported by The Scientific and Technological Research Council of Turkey (TUBITAK Project No. 113Y451).
Compliance with Ethical Standards
Conflict of interest
The authors declare that there are no conflicts of interest.
- 2.Liu, D., Zhang, R., Wu, H., Xu, D., Tang, Z., Yu, G., Xu, Z., Shen, Q.: Changes in biochemical and microbiological parameters during the period of rapid composting of dairy manure with rice chaff. Bioresour. Technol. 102, 9040–9049 (2011). https://doi.org/10.1016/j.biortech.2011.07.052 CrossRefGoogle Scholar
- 4.Nakasaki, K., Tran, L.T.H., Idemoto, Y., Abe, M., Rollon, A.P.: Comparison of organic matter degradation and microbial community during thermophilic composting of two different types of anaerobic sludge. Bioresour. Technol. 100, 676–682 (2009). https://doi.org/10.1016/j.biortech.2008.07.046 CrossRefGoogle Scholar
- 12.Xu, J., Lu, Y., Shan, G., Song, X., Huang, J., Li, Q.: Inoculation with compost-born thermophilic complex microbial consortium induced organic matters degradation while reduced nitrogen loss during co-composting of dairy manure and sugarcane leaves. Waste Biomass Valoriz. (2018). https://doi.org/10.1007/s12649-018-0293-y CrossRefGoogle Scholar
- 13.López-González, J.A., Vargas-García, MdelC., López, M.J., Suárez-Estrella, F., Jurado, MdelM., Moreno, J.: Biodiversity and succession of mycobiota associated to agricultural lignocellulosic waste-based composting. Bioresour. Technol. 187, 305–313 (2015). https://doi.org/10.1016/j.biortech.2015.03.124 CrossRefGoogle Scholar
- 14.Sundberg, C., Al-Soud, W.A., Larsson, M., Alm, E., Yekta, S.S., Svensson, B.H., Sørensen, S.J., Karlsson, A.: 454 pyrosequencing analyses of bacterial and archaeal richness in 21 full-scale biogas digesters. FEMS Microbiol. Ecol. 85, 612–626 (2013). https://doi.org/10.1111/1574-6941.12148 CrossRefGoogle Scholar
- 17.Tian, W., Sun, Q., Xu, D., Zhang, Z., Chen, D., Li, C., Shen, Q., Shen, B.: Succession of bacterial communities during composting process as detected by 16S rRNA clone libraries analysis. Int. Biodeterior. Biodegrad. 78, 58–66 (2013). https://doi.org/10.1016/j.ibiod.2012.12.008 CrossRefGoogle Scholar
- 18.Wang, X., Cui, H., Shi, J., Zhao, X., Zhao, Y., Wei, Z.: Bioresource technology relationship between bacterial diversity and environmental parameters during composting of different raw materials. Bioresour. Technol. 198, 395–402 (2015). https://doi.org/10.1016/j.biortech.2015.09.041 CrossRefGoogle Scholar
- 20.Zhang, L., Zhang, H., Wang, Z., Chen, G., Wang, L.: Dynamic changes of the dominant functioning microbial community in the compost of a 90-m3aerobic solid state fermentor revealed by integrated meta-omics. Bioresour. Technol. 203, 1–10 (2016). https://doi.org/10.1016/j.biortech.2015.12.040 CrossRefGoogle Scholar
- 21.Wang, C., Dong, D., Wang, H., Müller, K., Qin, Y., Wang, H., Wu, W.: Metagenomic analysis of microbial consortia enriched from compost: new insights into the role of Actinobacteria in lignocellulose decomposition. Biotechnol. Biofuels. 9, 1–17 (2016). https://doi.org/10.1186/s13068-016-0440-2 CrossRefGoogle Scholar
- 22.APHA/AWWA/WEF: Standard Methods for the Examination of Water and Wastewater. Stand. Methods. 541 (2012). ISBN 9780875532356Google Scholar
- 24.Liang, B., Wang, L.Y., Mbadinga, S.M., Liu, J.F., Yang, S.Z., Gu, J.D., Mu, B.Z.: Anaerolineaceae and Methanosaeta turned to be the dominant microorganisms in alkanes-dependent methanogenic culture after long-term of incubation. AMB Express 5, 37 (2015). https://doi.org/10.1186/s13568-015-0117-4 CrossRefGoogle Scholar
- 26.Oren, A.: The order Halanaerobiales, and the families Halanaerobiaceae and Halobacteroidaceae. In: The Prokaryotes, pp. 153–177. Springer, Berlin (2014)Google Scholar
- 27.Ozbayram, E.G., Kleinsteuber, S., Nikolausz, M., Ince, B., Ince, O.: Enrichment of lignocellulose-degrading microbial communities from natural and engineered methanogenic environments. Appl. Microbiol. Biotechnol. 102, 1035–1043 (2018). https://doi.org/10.1007/s00253-017-8632-7 CrossRefGoogle Scholar
- 33.López-González, J.A., Suárez-Estrella, F., Vargas-García, M.C., López, M.J., Jurado, M.M., Moreno, J.: Dynamics of bacterial microbiota during lignocellulosic waste composting: studies upon its structure, functionality and biodiversity. Bioresour. Technol. 175, 406–416 (2015). https://doi.org/10.1016/j.biortech.2014.10.123 CrossRefGoogle Scholar
- 34.Manz, W., Amann, R., Ludwig, W., Vancanneyt, M., Schleifer, K.H.: Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology. 142, 1097–1106 (1996). https://doi.org/10.1099/13500872-142-5-1097 CrossRefGoogle Scholar
- 36.Mandic-Mulec, I., Stefanic, P., van Elsas, J.D.: Ecology of Bacillaceae. In: The bacterial spore: from molecules to systems, pp. 59–85. American Society of Microbiology, Atlanta (2015)Google Scholar