Waste and Biomass Valorization

, Volume 10, Issue 11, pp 3271–3277 | Cite as

Effect of Co-digestion Ratio and Enzyme Treatment on Biogas Production from Grass Silage and Chicken Litter

  • N. BhatnagarEmail author
  • D. Ryan
  • R. Murphy
  • A. M. EnrightEmail author
Short Communication


Biomethane production potential was evaluated by anaerobic co-digestion of grass silage (GS) and chicken litter (CL) in batch experiments. The aim of this study was to identify optimum enzyme treatment and co-digestion ratio for these substrates. The study is divided into trial A and trial B for enzyme treatment and co-digestion respectively. Mixed substrates were utilised in both trials which were maintained for a period of 30 days in 125 mL serum bottles at 37 °C. Preliminary batch assays in trial A for biomethane potential determination were performed using a co-digestion ratio of 2:1 (GS:CL) treated with five different concentrations of enzyme (w/v)—0.05, 0.1, 0.25, 0.5 and 1%. The highest specific methane yield (SMY) of 61 L CH4/kg was observed for 0.1% (w/v) enzyme treatment. Trial B assays were conducted for nine different GS:CL ratios—0:1, 1:0, 1:1, 1:2, 1:3, 2:3, 3:2, 3:1, and 2:1 respectively. Highest SMY were recorded for the ratios 1:0, 1:1 and 1:3 of GS:CL which correspond to 124 L CH4/kg, 137 L CH4/kg and 135 L CH4/kg respectively.


Anaerobic co-digestion Biomethane potential Grass silage Chicken litter Enzyme treatment 



This study was supported by Alltech (Grant No. PES1162).


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.EnviroCOREInstitute of Technology CarlowCarlowIreland
  2. 2.AlltechDunboyneIreland

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