Abstract
Squid ovary (SO) was treated with 1% Alcalase or 1% papain at different hydrolysis times and foaming properties of resulting hydrolysates were investigated. SO hydrolysate (SOH) prepared by Alcalase for 30 min (SOH-Al-30) with degree of hydrolysis (DH) of 1.79% showed the highest foaming capacity (FC) (236%). For SOH prepared by papain for 15 min with DH of 0.21%, FC of 221% was attained. Foams from both SOH were collapsed by 27% when incubated at 25 °C for 60 min. The SOH-Al-30 powder showed high solubility and surface hydrophobicity. Particle size of SOH-Al-30 powder was slightly higher than that of squid ovary powder (SOP). FTIR spectra indicated that hydrolysis slightly affected the conformation and interaction of protein molecules. SOH-Al-30 pre-heated at 60 °C showed the highest FC (300%) and had the lowest liquid drainage when whipped for 4 min. Viscoelastic properties and microstructure of foam from pre-heated SOH-Al-30 were much improved than those from SOP. Thus, enzymatic hydrolysis having the controlled DH, followed by pre-heating at optimum temperature with the appropriate whipping time could improve foaming properties of squid ovary.
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Acknowledgements
This research was supported by the Higher Education Research Promotion and the Thailand’s Education Hub for Southern Region of ASEAN Countries (THE-AC, 2015) scholarship and Graduate School, Prince of Songkla University. The TRF Distinguished Research Professor Grant was also acknowledged.
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Singh, A., Benjakul, S. Effect of Partial Enzymatic Hydrolysis on Physicochemical and Foaming Properties of Ovary from Squid Loligo formosana. Waste Biomass Valor 10, 3351–3361 (2019). https://doi.org/10.1007/s12649-018-0348-0
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DOI: https://doi.org/10.1007/s12649-018-0348-0