Waste and Biomass Valorization

, Volume 9, Issue 6, pp 957–968 | Cite as

Hydrolysis, Detoxification and Alcoholic Fermentation of Hemicellulose Fraction from Palm Press Fiber

  • Philipe Luan Brito
  • Crisley Mara de Azevedo Ferreira
  • André Felipe Ferreira Silva
  • Lílian de Araújo Pantoja
  • David Lee Nelson
  • Alexandre Soares dos Santos
Original Paper

Abstract

The palm press fiber, resulting from the extraction of oil from the fruit of the oil palm (Elaeis guineensis) is an abundant agro-industrial co-product with a potential for development of biorefineries. This study evaluated the use of the hemicellulose fraction contained in the palm press fiber as a source of sugars for the production of bioethanol by Scheffersomyces stipitis. The optimal condition for hemicellulose hydrolysis, determined by response surface methodology, utilized 30% of dry biomass in 5% H2SO4 at 121 °C for 60 min, and resulted in removal of 88.4% of this polysaccharide. The soluble fraction recovered after the acid pretreatment, called hemicellulosic hydrolyzate, contained 83 g L−1 of reducing sugars. The hydrolyzate also contained 12 g L−1 of acetic acid, 489 mg L−1 of furfural and 46 mg L−1 of 5-hydroxymethylfurfural. The detoxification of the hydrolyzate with activated charcoal, overliming and a combination thereof was evaluated for removal of unwanted byproducts. The best detoxification treatment reduced the concentrations of phenolic compounds and furfural present in the hemicellulosic hydrolyzate by 96% and 99%, respectively. S. stipitis NRRLY 7124 and S. stipitis CBS 6054 were tested for the fermentation of the hydrolyzate. The highest yield of ethanol, 0.33 gethanol gsugar−1, was obtained with the NRRLY 7124 strain in the fermentation of the hydrolyzate detoxified by overliming. An estimated production of 12.1 L of ethanol per ton of palm press fiber derived solely from the hemicellulosic fraction was achieved.

Keywords

Bioethanol Biorefinery Elaeis guineensis Oil palm Pichia Scheffersomyces 

Notes

Acknowledgements

This work was supported by scholarships and financial assistance for research and development provided by the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the Coordenação de Aperfeiçoamento de Pessoal de Ensino Superior (CAPES). The strains of microorganisms utilized in this work were kindly given by Professor Thomas W. Jeffries of the University of Wisconsin. The palm press fiber was donated by the Agropalma, PA, Brazil.

Supplementary material

12649_2017_9882_MOESM1_ESM.docx (29 kb)
Supplementary material 1 (DOCX 29 KB)
12649_2017_9882_MOESM2_ESM.docx (45 kb)
Supplementary material 2 (DOCX 45 KB)

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Philipe Luan Brito
    • 1
  • Crisley Mara de Azevedo Ferreira
    • 2
  • André Felipe Ferreira Silva
    • 3
  • Lílian de Araújo Pantoja
    • 3
  • David Lee Nelson
    • 1
  • Alexandre Soares dos Santos
    • 4
  1. 1.Biofuels Graduate ProgramFederal University of Vales do Jequitinhonha e MucuriDiamantinaBrazil
  2. 2.Chemistry Graduate ProgramFederal University of Vales do Jequitinhonha e MucuriDiamantinaBrazil
  3. 3.Institute of Science and TechnologyFederal University of Vales do Jequitinhonha e MucuriDiamantinaBrazil
  4. 4.Department of Basic Sciences, Faculty of Biological Science and HealthFederal University of Vales do Jequitinhonha e MucuriDiamantinaBrazil

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