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Waste and Biomass Valorization

, Volume 10, Issue 5, pp 1419–1431 | Cite as

Cytotoxicity, Antioxidant and Antiviral Potential of Aqueous Extract from Nostoc muscorum Cultivated in Various Inexpensive Media

  • Emad A. ShalabyEmail author
  • Mohamed B. Atta
  • Ibtesam A. Sleem
  • Mousa A. Mohamed
  • David A. Lightfoot
  • Hany A. El-Shemy
Original Paper
  • 85 Downloads

Abstract

The genus Nostoc is a valuable source of a wide spectrum of natural products, which have many different biological activities such as antioxidant, anticancer, anti-HIV, antimalarial, antifungal and antimicrobial drugs. The current work was aimed to evaluate the biological activities of Nostoc muscorum microalga cultivated on different waste water samples (domestic, agricultural and industrial waste water). The algae was cultivated in different kinds of waste water; sterilized BG11 media, non-sterilized treated sewage waste water media (NS-TSW), sterilized treated sewage waste water media (S-TSW), 50% BG11 + 50% sterilized TSW media (50% S-TSW), non-sterilized industrial waste water media (NS-IW), sterilized industrial waste water media (S-IW), 50% BG11 + 50% sterilized industrial waste water media (50% S-IW), non-sterilized agriculture waste water media (NS-AW), sterilized agriculture waste water media (S-AW), and 50% BG11 + 50% sterilized AW media (50% S-AW). The experiment was conducted in triplicate and cultures were incubated at 25 ± 1 °C under continuous shaking (150 rpm) and illumination (2000 lx) for 15 days. The pigments content and biological activity include antioxidant (using diphenyl picryl hydrazyl and 2,2-azino-bis ethylbenzthiazoline-6-sulfonic acid radical methods), antiviral activity against Herpes simplex virus and the cytotoxicity for aqueous extracts in vivo and in vitro assays were determined. The given results reported that use of waste water as media for cultivation of microalgae is a suitable and inexpensive method when compared with ordinary cultivation methods. Also We can conclude that the algal species has high ability to produce active ingredients used as antioxidants and antivirals especially from algae cultivated in S-TSW, 50% S-TSW, NS-AW and 50% S-AW without any toxicity in mice.

Keywords

Antioxidant Antiviral Cytotoxicity In vivo toxicity Nostoc muscorum Waste water 

Abbreviations

ABTS

2,2-Azino-bis ethylbenzthiazoline-6-sulfonic acid

AW

Agricultural waste water

ALT

Alanine aminotransferase

APC

Allophycocyanin

AST

Aspartate aminotransferase

BHA

Butylated hydroxyl anisol

BUN

Blood urea nitrogen

MTT

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

DPPH

Diphenyl picryl hydrazyl

HDL

High density lipoprotein

GLU

Glucose

IW

Industrial waste water

LDL

Low density lipoprotein

PE

Phycoerythrin

TSW

Treated sewage waste water

Notes

Acknowledgements

The authors are thankful to Faculty of Agriculture in University of Cairo and University of Tanta for providing the facilities.

Author Contributions

Conceived and designed the experiments: MBA, EAS, MAM. Performed the experiments: IAS, EAS, GIM Collection of data: IAS, MBA, EAS, MAM. Analyzed the data: MBA, EAS, IAS, HAE. DAL Contributed reagents/materials/analysis tools: MBA, IAS, EAS, GIM. Wrote the paper: EAS, HAE, DAL. Revising of manuscript: EAS, HAE, DAL.

Funding

This work was partially supported by a grant from the Science and Technology Development Fund (STDF-Project ID: 312), Cairo, Egypt.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interests.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Emad A. Shalaby
    • 1
    Email author
  • Mohamed B. Atta
    • 2
  • Ibtesam A. Sleem
    • 2
  • Mousa A. Mohamed
    • 2
  • David A. Lightfoot
    • 3
  • Hany A. El-Shemy
    • 1
  1. 1.Biochemistry Department, Faculty of AgricultureCairo UniversityGizaEgypt
  2. 2.Food Science and Technology Department, Faculty of AgricultureTanta UniversityTantaEgypt
  3. 3.Genomics Core-FacilitySouthern Illinois University at CarbondaleCarbondleUSA

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