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Waste and Biomass Valorization

, Volume 10, Issue 5, pp 1131–1140 | Cite as

Effect of Alkali Treatment Combined with High Pressure on Extraction Efficiency of β-d-Glucan from Spent Brewer’s Yeast

  • Xiaoli Tian
  • Ping Yang
  • Wenxia JiangEmail author
Original Paper
  • 64 Downloads

Abstract

β-d-glucan, an important constituent of spent brewer’s yeast cell walls, shows numerous physiological functions, leading to the great potential to be used in food industry. Conventional isolation method of β-d-glucan (alkali treatment at ordinary pressure, ATOP) requires high alkali dosage and excessively long extraction time. In this paper, we found that combination of high pressure treatment and alkali treatment could reduce alkali dosage and significantly shorten processing time. Transmission electron micrographs of yeast cells showed that combination of high pressure treatment and alkali treatment made yeast cell walls swell significantly and become loose, which increased the contact area with alkali solution and contributed to the efficient removal of other impurities. Accordingly, an innovative isolation method (i.e., alkali treatment at high pressure, ATHP) of β-d-glucan from spent brewer’s yeast was developed in this study. The optimal conditions of 0.85% alkali concentration, 6.5:1 liquid–solid ratio, 108 °C temperature (pressure, 0.039 MPa), and 5 min time were obtained by using single-factor experiments and response surface methodology. Under these conditions, β-d-glucan content and extraction rate were 78.11 and 78.38%, respectively. NMR spectra confirmed that glucan prepared by ATHP is a polymer of β-(1,3) linked glucose with β-(1,6) branches.

Keywords

β-d-Glucan Spent brewer’s yeast Alkali treatment at high pressure Saccharomyces cerevisiae 

Notes

Acknowledgements

This project was financially supported by Program for the National High-tech Research and Development Plan (2012AA021403).

References

  1. 1.
    Liu, H., Lin, J.P., Cen, P.L., Pan, Y.J.: Co-production of S-adenosyl-L-methionine and glutathione from spent brewer’s yeast cells. Process Biochem. 39(12), 1993–1997 (2004)CrossRefGoogle Scholar
  2. 2.
    Jiang, M., Chen, K., Liu, Z., Wei, P., Ying, H., Chang, H.: Succinic acid production by Actinobacillus succinogenes using spent brewer’s yeast hydrolysate as a nitrogen source. Appl. Biochem. Biotechnol. 160(1), 244–254 (2010)CrossRefGoogle Scholar
  3. 3.
    Shotipruk, A., Kittianong, P., Suphantharika, M., Muangnapoh, C.: Application of rotary microfiltration in debittering process of spent brewer’s yeast. Bioresour. Technol. 96, 1851–1859 (2005)CrossRefGoogle Scholar
  4. 4.
    Brady, D., Stoll, A.D., Starke, L., Duncan, J.R.: Chemical and enzymatic extraction of heavy metal binding polymers from isolated cell walls of Saccharomyces cerevisiae. Biotechnol. Bioeng. 44(3), 297–302 (1994)CrossRefGoogle Scholar
  5. 5.
    Sugawara, T., Takahashi, S., Osumi, M., Ohno, N.: Refinement of the structures of cell-wall glucans of Schizosaccharomyces pombe by chemical modification and NMR spectroscopy. Carbohydr. Res. 339(13), 2255–2265 (2004)CrossRefGoogle Scholar
  6. 6.
    Hofer, M., Pospisil, M.: Glucan as stimulator of hematopoiesis in normal and gamma-irradiated mice. A survey of the authors’ results. Int. J. Immunopharmacol. 19(9–10), 607–609 (1997)CrossRefGoogle Scholar
  7. 7.
    Kogan, G.: (1→3, 1→6)-β-D-Glucans of yeasts and fungi and their biological activity. Stud. Nat. Prod. Chem. 23, 107–152 (2000)CrossRefGoogle Scholar
  8. 8.
    Thammakiti, S., Suphantharika, M., Phaesuwan, T., Verduyn, C.: Preparation of spent brewer’s yeast β-glucans for potential applications in the food industry. Int J Food Sci. Technol. 39(1), 21–29 (2004)CrossRefGoogle Scholar
  9. 9.
    Santipanichwong, R., Suphantharika, M.: Carotenoids as colorants in reduced-fat mayonnaise containing spent brewer’s yeast beta-glucan as a fat replacer. Food Hydrocolloid. 21(4), 565–574 (2007)CrossRefGoogle Scholar
  10. 10.
    Thanardkit, P., Khunrae, P., Suphantharika, M., Verduyn, C.: Glucan from spent brewer’s yeast: preparation, analysis and use as a potential immunostimulant in shrimp feed. World J. Microbiol. Biotechnol. 18(6), 527–539 (2002)CrossRefGoogle Scholar
  11. 11.
    Yiannikouris, A., Francois, J., Poughon, L., Dussap, C.G., Bertin, G., Jeminet, G., Jouany, J.P.: Alkali extraction of β-D-glucans from Saccharomyces cerevisiae cell wall and study of their adsorptive properties toward zearalenone. J. Agric. Food Chem. 52(11), 3666–3673 (2004)CrossRefGoogle Scholar
  12. 12.
    Huang, G.L.: Extraction of two active polysaccharides from the yeast cell wall. Z. Naturforsch. C. 63(11–12), 919–921 (2008)CrossRefGoogle Scholar
  13. 13.
    Freimund, S., Sauter, M., Kappeli, O., Dutler, H.: A new non-degrading isolation process for 1,3-β-D-glucan of high purity from baker’s yeast Saccharomyces cerevisiae. Bioresour. Technol. 54(2), 159–171 (2003)Google Scholar
  14. 14.
    Liu, X.Y., Wang, Q., Cui, S.W., Liu, H.Z.: A new isolation method of β-D-glucans from spent yeast Saccharomyces cerevisiae. Food Hydrocolloid. 22(2), 239–247 (2008)CrossRefGoogle Scholar
  15. 15.
    AOAC.: Official methods of analysis, methods 955.04, 920.39 (15th edn.). Association of Official Analytical Chemists, Arlington VA (1990)Google Scholar
  16. 16.
    Dallies, N., Francois, J., Paquet, V.: A new method for quantitative determination of polysaccharides in the yeast cell wall. Application to the cell wall defective mutants of Saccharomyces cerevisiae. Yeast. 14(14), 1297–1306 (1998)CrossRefGoogle Scholar
  17. 17.
    Giovani, G., Rosi, I.: Release of cell wall polysaccharides from Saccharomyces cerevisiae thermosensitive autolytic mutants during alcoholic fermentation. Int. J. Food Microbiol. 116(1), 19–24 (2007)CrossRefGoogle Scholar
  18. 18.
    Jaehrig, S.C., Rohn, S., Kroh, L.W., Fleischer, L.G., Kurz, T.: In vitro potential antioxidant activity of (1→3),(1→6)-β-D-glucan and protein fractions from Saccharomyces cerevisiae cell walls. J. Agric. Food Chem. 55(12), 4710–4716 (2007)CrossRefGoogle Scholar
  19. 19.
    Walther, P., Muller, M.: Double-layer coating for field-emission cryo-scanning electron microscopy - Present state and applications. Scanning. 19(5), 343–348 (1997)CrossRefGoogle Scholar
  20. 20.
    Manners, D.J., Masson, A.J., Patterson, J.C.: The structure of a β-(1→3)-D-glucan from yeast cell walls. J. Biochem. 135, 19–30 (1973)CrossRefGoogle Scholar
  21. 21.
    Choi, S.J., Chung, B.H.: Simultaneous production of invertase and yeast extract from baker’s yeast. Korean J. Biotechnol. Bioeng. 13, 308–311 (1998)Google Scholar
  22. 22.
    Chae, H.J., Joo, H., In, M.J.: Utilization of brewer’s yeast cells for the production of food-grade yeast extract. Part 1. effects of different enzymatic treatments on solid and protein recovery and flavor characteristics. Bioresour. Technol. 76(3), 253–258 (2001)CrossRefGoogle Scholar
  23. 23.
    Magnani, M., Calliari, C.M., de Macedo, F.C., Mori, M.P., Colus, I.M.D., Castro-Gomez, R.J.H.: Optimized methodology for extraction of (1→3)(1→6)-β-D-glucan from Saccharomyces cerevisiae and in vitro evaluation of the cytotoxicity and genotoxicity of the corresponding carboxymethyl derivative. Carbohydr. Polym. 78(4), 658–665 (2009)CrossRefGoogle Scholar
  24. 24.
    Kath, F., Kulicke, W.M.: Mild enzymatic isolation of mannan and glucan from yeast Saccharomyces cerevisiae. Angew. Makromol. Chem. 268, 59–68 (1999)CrossRefGoogle Scholar
  25. 25.
    Misaki, A., Johnson, J. Jr., Kirkwood, S., Scaletti, J.V., Smith, F.: Structure of the cell-wall glucan of yeast (Saccharomyces cerevisiae). Carbohydr. Res. 6, 150–164 (1968)CrossRefGoogle Scholar
  26. 26.
    Jamas, S., Easson, D.D., Ostroff, G.R.: Glucan preparation. US Patent. 5,622,939 (1997)Google Scholar
  27. 27.
    Suphantharika, M., Khunrae, P., Thanardkit, P., Verduyn, C.: Preparation of spent brewer’s yeast β-glucans with a potential application as an immunostimulant for black tiger shrimp, Penaeus monodon. Bioresour. Technol. 88(1), 55–60 (2003)CrossRefGoogle Scholar
  28. 28.
    Li, Q.H., Fu, C.L.: Application of response surface methodology for extraction optimization of germinant pumpkin seeds protein. Food Chem. 92(4), 701–706 (2005)CrossRefGoogle Scholar
  29. 29.
    Kim, Y.T., Kim, E.H., Cheong, C., Williams, D.L., Kim, C.W., Lim, S.T.: Structural characterization of β-D-(1→3, 1→6)-linked glucans using NMR spectroscopy. Carbohydr. Res. 328(3), 331–341 (2000)CrossRefGoogle Scholar

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Authors and Affiliations

  1. 1.Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjinChina
  2. 2.College of Food Engineering and BiotechnologyTianjin University of Science & TechnologyTianjinChina

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