Effect of Alkali Treatment Combined with High Pressure on Extraction Efficiency of β-d-Glucan from Spent Brewer’s Yeast
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β-d-glucan, an important constituent of spent brewer’s yeast cell walls, shows numerous physiological functions, leading to the great potential to be used in food industry. Conventional isolation method of β-d-glucan (alkali treatment at ordinary pressure, ATOP) requires high alkali dosage and excessively long extraction time. In this paper, we found that combination of high pressure treatment and alkali treatment could reduce alkali dosage and significantly shorten processing time. Transmission electron micrographs of yeast cells showed that combination of high pressure treatment and alkali treatment made yeast cell walls swell significantly and become loose, which increased the contact area with alkali solution and contributed to the efficient removal of other impurities. Accordingly, an innovative isolation method (i.e., alkali treatment at high pressure, ATHP) of β-d-glucan from spent brewer’s yeast was developed in this study. The optimal conditions of 0.85% alkali concentration, 6.5:1 liquid–solid ratio, 108 °C temperature (pressure, 0.039 MPa), and 5 min time were obtained by using single-factor experiments and response surface methodology. Under these conditions, β-d-glucan content and extraction rate were 78.11 and 78.38%, respectively. NMR spectra confirmed that glucan prepared by ATHP is a polymer of β-(1,3) linked glucose with β-(1,6) branches.
Keywordsβ-d-Glucan Spent brewer’s yeast Alkali treatment at high pressure Saccharomyces cerevisiae
This project was financially supported by Program for the National High-tech Research and Development Plan (2012AA021403).
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