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Waste and Biomass Valorization

, Volume 10, Issue 3, pp 641–653 | Cite as

Extraction of Betacyanin and Betaxanthin Pigments from Red Beetroots by Chitosan Extracted from Shrimp Wastes

  • Mohammad Sadegh Tanabtabzadeh
  • Vahid JavanbakhtEmail author
  • Amir Hossain Golshirazi
Original Paper
  • 152 Downloads

Abstract

Red beetroot as the main source of natural red dye is rich in betalain pigments, which are divided into betacyanins and betaxanthins with antioxidant, antimicrobial and antiviral activity. In this study, chitosan as a deacetylated derivative of chitin, non-toxic, biodegradable and biocompatible polysaccharide, was extracted from shrimp wastes by demineralization, deproteinization and deacetylation processes. The extracted chitosan was characterized by SEM and FTIR analyses. The weight efficiency of chitosan from shrimp wastes and the degree of deacetylation were 26.25 and 86%, respectively. The extracted chitosan was used to betacyanin and betaxanthin adsorption from red beetroots. Betacyanin and betaxanthin adsorption experiments were conducted based on a rotatable central composite design and analyzed using response surface methodology for three independent factors, initial solution pH (3–7), initial concentration (50–150 mg/L) and adsorbent amount (0.01–0.05 g). The optimum adsorption capacity for the betacyanin and betaxanthin pigments were 144.15 and 114.50 mg/g, respectively. The chitosan extracted from shrimp wastes could be used as a proper and available adsorbent for adsorption of betacyanin and betaxanthin pigments from beetroot plant.

Keywords

Shrimp wastes Chitosan Beetroot 

Notes

Acknowledgements

Financial support of this work by ACECR Institute of Higher Education (Isfahan Branch) is gratefully appreciated.

References

  1. 1.
    Al Sagheer, F., Al-Sughayer, M., Muslim, S., Elsabee, M.Z.: Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf. Carbohydr. Polym. 77(2), 410–419 (2009)CrossRefGoogle Scholar
  2. 2.
    Kumari, S., Rath, P., Kumar, A.S.H., Tiwari, T.: Extraction and characterization of chitin and chitosan from fishery waste by chemical method. Environ. Technol. Innov. 3, 77–85 (2015)CrossRefGoogle Scholar
  3. 3.
    Park, B.K., Kim, M.-M.: Applications of chitin and its derivatives in biological medicine. Int. J. Mol. Sci. 11(12), 5152–5164 (2010)CrossRefGoogle Scholar
  4. 4.
    Cira, L.A., Huerta, S., Hall, G.M., Shirai, K.: Pilot scale lactic acid fermentation of shrimp wastes for chitin recovery. Process Biochem. 37(12), 1359–1366 (2002)CrossRefGoogle Scholar
  5. 5.
    Mahlous, M., Tahtat, D., Benamer, S., Khodja, A.N.: Gamma irradiation-aided chitin/chitosan extraction from prawn shells. Nucl. Instr. Meth. Phys. Res. Sect. B 265(1), 414–417 (2007)CrossRefGoogle Scholar
  6. 6.
    Abdou, E.S., Nagy, K.S., Elsabee, M.Z.: Extraction and characterization of chitin and chitosan from local sources. Bioresour. Technol. 99(5), 1359–1367 (2008)CrossRefGoogle Scholar
  7. 7.
    Shushizadeh, M.R., Pour, E.M., Zare, A., Lashkari, Z.: Persian gulf β-chitin extraction from sepia pharaonis sp. cuttlebone and preparation of its derivatives. Bioact. Carbohydr. Diet. Fibre 6(2), 133–142 (2015)CrossRefGoogle Scholar
  8. 8.
    Sonaje, K., Chen, Y.-J., Chen, H.-L., Wey, S.-P., Juang, J.-H., Nguyen, H.-N., Hsu, C.-W., Lin, K.-J., Sung, H.-W.: Enteric-coated capsules filled with freeze-dried chitosan/poly (γ-glutamic acid) nanoparticles for oral insulin delivery. Biomaterials 31(12), 3384–3394 (2010)CrossRefGoogle Scholar
  9. 9.
    Wruss, J., Waldenberger, G., Huemer, S., Uygun, P., Lanzerstorfer, P., Müller, U., Höglinger, O., Weghuber, J.: Compositional characteristics of commercial beetroot products and beetroot juice prepared from seven beetroot varieties grown in Upper Austria. J. Food Compos. Anal. 42, 46–55 (2015)CrossRefGoogle Scholar
  10. 10.
    López, N., Puértolas, E., Condón, S., Raso, J., Alvarez, I.: Enhancement of the extraction of betanine from red beetroot by pulsed electric fields. J. Food Eng. 90(1), 60–66 (2009)CrossRefGoogle Scholar
  11. 11.
    De Azeredo, H.M.C., Pereira, A.C., De Souza, A.C.R., Gouveia, S.T., Mendes, K.C.B.: Study on efficiency of betacyanin extraction from red beetroots. Int. J. Food Sci. Technol. 44(12), 2464–2469 (2009)CrossRefGoogle Scholar
  12. 12.
    Kovačević, S.Z., Tepić, A.N., Jevrić, L.R., Podunavac-Kuzmanović, S.O., Vidović, S.S., Šumić, Z.M., Ilin, Ž.M.: Chemometric guidelines for selection of cultivation conditions influencing the antioxidant potential of beetroot extracts. Comput. Electron. Agric. 118, 332–339 (2015)CrossRefGoogle Scholar
  13. 13.
    Marszałek, K., Krzyżanowska, J., Woźniak, Ł., Skąpska, S.: Kinetic modelling of polyphenol oxidase, peroxidase, pectin esterase, polygalacturonase, degradation of the main pigments and polyphenols in beetroot juice during high pressure carbon dioxide treatment. LWT-Food Sci. Technol. 85, 412–417 (2016)CrossRefGoogle Scholar
  14. 14.
    Sawicki, T., Bączek, N., Wiczkowski, W.: Betalain profile, content and antioxidant capacity of red beetroot dependent on the genotype and root part. J. Funct. Foods 27, 249–261 (2016)CrossRefGoogle Scholar
  15. 15.
    Zvitov, R., Nussinovitch, A.: Low DC electrification of gel-plant tissue ‘sandwiches’ facilitates extraction and separation of substances from Beta vulgaris beetroots. Food Hydrocolloids 19(6), 997–1004 (2005)CrossRefGoogle Scholar
  16. 16.
    Tran, T.N., Athanassiou, A., Basit, A., Bayer, I.S.: Starch-based bio-elastomers functionalized with red beetroot natural antioxidant. Food. Chem. 216, 324–333 (2017)CrossRefGoogle Scholar
  17. 17.
    Wootton-Beard, P.C., Ryan, L.: A beetroot juice shot is a significant and convenient source of bioaccessible antioxidants. J. Funct. Foods 3(4), 329–334 (2011)CrossRefGoogle Scholar
  18. 18.
    Clifford, T., Howatson, G., West, D.J., Stevenson, E.J.: The potential benefits of red beetroot supplementation in health and disease. Nutrients 7(4), 2801–2822 (2015)CrossRefGoogle Scholar
  19. 19.
    Raikos, V., McDonagh, A., Ranawana, V., Duthie, G.: Processed beetroot (Beta vulgaris L.) as a natural antioxidant in mayonnaise: effects on physical stability, texture and sensory attributes. Food Sci. Hum. Wellness 5(4), 191–198 (2016)CrossRefGoogle Scholar
  20. 20.
    Bazaria, B., Kumar, P.: Effect of whey protein concentrate as drying aid and drying parameters on physicochemical and functional properties of spray dried beetroot juice concentrate. Food Biosci. 14, 21–27 (2016)CrossRefGoogle Scholar
  21. 21.
    Jajja, A., Sutyarjoko, A., Lara, J., Rennie, K., Brandt, K., Qadir, O., Siervo, M.: Beetroot supplementation lowers daily systolic blood pressure in older, overweight subjects. Nutr. Res. 34(10), 868–875 (2014)CrossRefGoogle Scholar
  22. 22.
    Younes, I., Rinaudo, M.: Chitin and chitosan preparation from marine sources. Structure, properties and applications. Mar. Drugs 13(3), 1133–1174 (2015)CrossRefGoogle Scholar
  23. 23.
    de Queiroz Antonino, R.S.C.M., Fook, Lia, de Oliveira Lima, B.R.P., de Farias Rached, V.A., Lima, R.Í., da Silva Lima, E.P.N., Peniche Covas, R.J., Lia, C.A., Fook, M.V.: Preparation and characterization of chitosan obtained from shells of shrimp (Litopenaeus vannamei Boone). Mar. Drugs 15(5), 141 (2017)CrossRefGoogle Scholar
  24. 24.
    Domszy, J.G., Roberts, G.A.: Evaluation of infrared spectroscopic techniques for analysing chitosan. Macromol. Chem. Phys. 186(8), 1671–1677 (1985)CrossRefGoogle Scholar
  25. 25.
    Kousalya, G., Gandhi, M.R., Viswanathan, N., Meenakshi, S.: Preparation and metal uptake studies of modified forms of chitin. Int. J. Biol. Macromol. 47(5), 583–589 (2010)CrossRefGoogle Scholar
  26. 26.
    Longhinotti, E., Pozza, F., Furlan, L., Sanchez, M.d.N.d.M., Klug, M., Laranjeira, M., Fávere, V.T.: Adsorption of anionic dyes on the biopolymer chitin. J. Braz. Chem. Soc. 9(5), 435–440 (1998)CrossRefGoogle Scholar
  27. 27.
    Amini, M., Younesi, H., Bahramifar, N., Lorestani, A.A.Z., Ghorbani, F., Daneshi, A., Sharifzadeh, M.: Application of response surface methodology for optimization of lead biosorption in an aqueous solution by Aspergillus niger. J. Hazard. Mater. 154(1), 694–702 (2008)CrossRefGoogle Scholar
  28. 28.
    Ngah, W.W., Endud, C., Mayanar, R.: Removal of copper (II) ions from aqueous solution onto chitosan and cross-linked chitosan beads. React. Funct. Polym. 50(2), 181–190 (2002)CrossRefGoogle Scholar
  29. 29.
    Sankararamakrishnan, N., Dixit, A., Iyengar, L., Sanghi, R.: Removal of hexavalent chromium using a novel cross linked xanthated chitosan. Bioresour. Technol. 97(18), 2377–2382 (2006)CrossRefGoogle Scholar
  30. 30.
    Kumar, M., Tripathi, B.P., Shahi, V.K.: Crosslinked chitosan/polyvinyl alcohol blend beads for removal and recovery of Cd (II) from wastewater. J. Hazard. Mater. 172(2), 1041–1048 (2009)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Mohammad Sadegh Tanabtabzadeh
    • 1
  • Vahid Javanbakht
    • 1
    Email author
  • Amir Hossain Golshirazi
    • 1
  1. 1.ACECR Institute of Higher Education (Isfahan Branch)IsfahanIran

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