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Recycled Paper Sludge Microbial Community as a Potential Source of Cellulase and Xylanase Enzymes

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Abstract

Waste disposal from paper and pulp industry is of great environmental concern. Sludge from recycled paper mills favors colonization with different microbial species due to its chemical composition. In this environment, the microbiota can express enzymes that hydrolyze celluloses and hemicelluloses, such as cellulases, β-glucosidases, and xylanases. Understanding microbiota living in such disposal may give directions to what kind of enzymes or bioproducts may be possible to obtain from this microorganism and how to deal with the waste during the process. We have accessed microbial community associated with two steps in the recycled paper sludge generation by high-throughput DNA sequencing: fibrous residue, and wastewater sludge. Bacteroidetes, Proteobacteria and Firmicutes phyla represented more than 96% of the total. From the eukaryote perspective, Vorticella was the most frequent in fibrous residue samples while Acanthamoeba was the most common on wastewater sludge samples. Among the isolates cellulase activity was detectable only from fungi isolates. Half of the bacterial isolates presented xylanase activity, and most of them in high level of activity. Both a metabarcoding and culturing approaches are valuable tools for enzymatic screening in industrial waste disposal.

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Acknowledgements

The authors acknowledge Brazilian Agricultural Research Corporation (EMBRAPA) for funding the project “Energetic Forest-Sustainable Production and Conversion of Biomass into Energy” under which this study has been prepared; National Research Council for Scientific and Technological Development (CNPq) and Foundation for Research Support of Santa Catarina (FAPESC) for the fellowships (LBBT and KGHH), respectively.

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Authors and Affiliations

  1. Centro de Ciências Tecnológicas, PPGEA, FURB, Rua São Paulo 3250, 89030-000, Blumenau, SC, Brazil

    Kássia G. H. Heinz & Lorena B. B. Tavares

  2. EMBRAPA Florestas, Estrada da Ribeira km 111, P.O. Box 319, 83411-000, Colombo, PR, Brazil

    Patrícia R. S. Zanoni

  3. Instituto do Petróleo e dos Recursos Naturais, PUCRS, Av. Ipiranga, 6681, Prédio 96J, 90619-900, Porto Alegre, RS, Brazil

    Rafael R. Oliveira, Renata Medina-Silva & Adriana Giongo

  4. Faculdade de Biociências, PUCRS, Av. Ipiranga, 6681, Prédio 12C, 90619-900, Porto Alegre, RS, Brazil

    Renata Medina-Silva, Taiz L. L. Simão, Fernanda J. Trindade & Leandro M. Pereira

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  1. Kássia G. H. Heinz
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  2. Patrícia R. S. Zanoni
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Heinz, K.G.H., Zanoni, P.R.S., Oliveira, R.R. et al. Recycled Paper Sludge Microbial Community as a Potential Source of Cellulase and Xylanase Enzymes. Waste Biomass Valor 8, 1907–1917 (2017). https://doi.org/10.1007/s12649-016-9792-x

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