Waste and Biomass Valorization

, Volume 7, Issue 3, pp 513–525 | Cite as

Deoiled Rice Bran: A Substrate for Co-Production of a Consortium of Hydrolytic Enzymes by Aspergillus niger P-19

Original Paper
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Abstract

With the aim to utilize deoiled rice bran, an agro-industrial waste, as a feedstock for the co-production of multiple carbohydrases, a fungal strain was isolated which could utilize DORB to co-produce a consortium of cellulases, hemicellulases, pectinase and amylases and was named as Aspergillus niger P-19 after molecular identification. Further, optimization for the co-production of all the enzymes was carried out by one factor at a time approach. Time profile studies of the production of enzymes revealed that 5th day of incubation was best suited for the extraction of enzymes. An initial solid to moisture ratio of 1:1.5 and an inoculum size of 5 × 107 spores gds−1 were found to be optimum for maximum productivities. Enzyme yields were significantly improved with the exogenous supplementation of carbon source, nitrogen source, surfactants and lignocellulosic inducers. This is the first report of its kind where DORB has been utilized for the co-production and co-optimization of eight different enzymes which can have a potential application in biofuel industry as the enzyme preparation could effectively hydrolyze steam pre-treated DORB releasing a total reducing sugars of 356.17 ± 9.58 mg gds−1.

Keywords

Deoiled rice bran Cellulases Hemicellulases Pectinase Amylases Enzymes Biofuels 
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Notes

Acknowledgments

Authors are highly thankful for the financial assistance provided by Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India and Department of Science and Technology (DST). Junior Research Fellowship awarded under DST-INSPIRE scheme to Ms. Priya is also acknowledged.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12649_2015_9477_MOESM1_ESM.pdf (75 kb)
Fig. 1 Phylogenetic tree for strain P-19 and related fungal strains based on 18S rDNA sequencing (PDF 75 kb)

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© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of MicrobiologyPanjab UniversityChandigarhIndia
  2. 2.Department of BiotechnologyDAV CollegeChandigarhIndia

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