Indian Journal of Physics

, Volume 89, Issue 10, pp 997–1005 | Cite as

Formation of silver nanoparticle at phospholipid template using Langmuir–Blodgett technique and its Surface-enhanced Raman Spectroscopy application

  • M. Mahato
  • R. Sarkar
  • P. Pal
  • G. B. Talapatra
Original paper


The biosynthesis of metal nanoparticle and their suitable assembly has recently gained tremendous interest for its application in biomedical arena such as substrates for surface-enhanced Raman scattering and others. In this article, an easy, low-cost, fast, bio-friendly and toxic-reducing agent free protocol has been described for the preparation of silver nanoparticle film using biocompatible 1,2-dipalmitoyl-sn-glycero-3-phosphocholine phospholipid Langmuir monolayer template. Interactions, docking and attachment of silver ions to the above-mentioned phospholipid monolayer have been studied by surface pressure–area isotherm and compressibility analysis at the air–water interface. We have deposited the Langmuir–Blodgett monolayer/multilayer containing silver nanoparticle onto glass/SiO2/quartz substrates. The formation of phospholipid–silver nanoparticle complex in Langmuir–Blodgett film has been characterized by field emission-scanning electron microscopy and high-resolution tunneling electron microscopy images. We have applied this deposited film as a substrate for surface-enhanced Raman scattering application using rhodamine 123 to understand the existence of the surface plasmon activity of silver nanoparticle.


AgNP Biosynthesis Phospholipid Langmuir–Blodgett technique 


87.85.jj 68.47.Pe 78.67.Bf 



We thank DST, Government of India (Project No. SR/S2/CMP-0079/2010(G)) for partial financial support. Thanks also go to the authority of the IACS for providing central instrumental facilities of FE-SEM, HR-TEM, FTIR and micro-Raman system.


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Copyright information

© Indian Association for the Cultivation of Science 2015

Authors and Affiliations

  • M. Mahato
    • 1
  • R. Sarkar
    • 2
  • P. Pal
    • 3
  • G. B. Talapatra
    • 3
  1. 1.Department of Basic Sciences and Social Sciences, School of TechnologyNorth Eastern Hill UniversityShillongIndia
  2. 2.Department of PhysicsJogesh Chandra Chaudhuri CollegeKolkataIndia
  3. 3.Department of SpectroscopyIndian Association for the Cultivation of ScienceJadavpur, KolkataIndia

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