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Neurotoxicity Research

, Volume 35, Issue 2, pp 410–420 | Cite as

Mild Exercise Differently Affects Proteostasis and Oxidative Stress on Motor Areas During Neurodegeneration: A Comparative Study of Three Treadmill Running Protocols

  • Karla P. Melo
  • Carolliny M. Silva
  • Michael F. Almeida
  • Rodrigo S. Chaves
  • Tania Marcourakis
  • Sandra M. Cardoso
  • Marilene Demasi
  • Luis E. S. Netto
  • Merari F. R. FerrariEmail author
ORIGINAL ARTICLE

Abstract

Proteostasis and oxidative stress were evaluated in motor cortex and spinal cord of aged Lewis rats exposed to 1 mg/kg/day of rotenone during 4 or 8 weeks, prior or after practicing three protocols of mild treadmill running. Results demonstrated that exercise done after the beginning of neurodegeneration reverted the increased oxidative stress (measured by H2O2 levels and SOD activity), increased neuron strength, and improved proteostasis in motor cortex. Spinal cord was not affected. Treadmill running practiced before neurodegeneration protected cortical motor neurons of the rotenone-exposed rats; but in this case, oxidative stress was not altered, whereas proteasome activity was increased and autophagy decreased. Spinal cord was not protected when exercise was practiced before neurodegeneration. Prolonged treadmill running (10 weeks) increased oxidative stress, autophagy, and proteasome activity, whereas neuron viability was decreased in motor cortex. In spinal cord, this protocol decreased oxidative stress and increased proteasome activity. Major conclusions were that treadmill running practiced before or after the beginning of neurodegeneration may protect motor cortex neurons, whereas prolonged mild running seems to be beneficial for spinal cord.

Keywords

Spinal cord Motor cortex Autophagy Rotenone Physical exercise Proteasome 

Notes

Acknowledgements

The authors are grateful to Professors Debora R. Fior-Chadi and Edilamar Menezes and Dr. Tiago Fernandes for helping with treadmill running. This study was supported by research grants from Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) (2011/06434-7; 2013/08028-1; 2015/18961-2), Conselho Nacional de desenvolvimento Cientifico e Tecnológico (CNPq) (471999/2013-0; 401670/2013-9) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). K.P.M. received fellowships from CNPq and FAPESP (2017/14273-0). M.F.A, C.M.S. and R.S.C. received fellowships from FAPESP (2011/15281-0, 2011/15283-2, and 2011/00478-2, respectively).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Karla P. Melo
    • 1
  • Carolliny M. Silva
    • 1
  • Michael F. Almeida
    • 1
  • Rodrigo S. Chaves
    • 1
  • Tania Marcourakis
    • 2
  • Sandra M. Cardoso
    • 3
  • Marilene Demasi
    • 4
  • Luis E. S. Netto
    • 1
  • Merari F. R. Ferrari
    • 1
    Email author
  1. 1.Departamento de Genetica e Biologia Evolutiva, Instituto de BiocienciasUniversidade de Sao PauloSao PauloBrazil
  2. 2.Department of Clinical and Toxicological Analyses, School of Pharmaceutical SciencesUniversity of Sao PauloSao PauloBrazil
  3. 3.Center for Neuroscience and Cell Biology and Faculty of MedicineUniversity of CoimbraCoimbraPortugal
  4. 4.Laboratorio de Bioquimica e BiofisicaInstituto ButantanSao PauloBrazil

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