Neurotoxicity Research

, Volume 33, Issue 2, pp 353–361 | Cite as

The Role of HINT1 in Methamphetamine-Induced Conditioned Place Preference

  • Ju-ping Li
  • Peng Liu
  • Gang Lei
  • Zheng Chu
  • Fei Liu
  • Chao-feng Shi
  • Yong-hui DangEmail author
  • Teng ChenEmail author


Drug addiction is a chronically relapsing disorder in humans; yet, the underlying mechanism remained unclear. Recent studies suggested that the histidine triad nucleotide binding protein 1 (HINT1) may play significant roles in diverse neuropsychiatric diseases including drug addiction. In our present study, we used different batches of mice to establish the different stages of methamphetamine (METH)-induced conditioned place preference (CPP) to explore the dynamic changes throughout the process of addiction in different brain regions, including prefrontal cortex (PFC), nucleus accumbens (NAc), corpus striatum (CPu), and hippocampus (Hip). We found that in NAc of the METH group mice, the HINT1 expression level initially increased after acquisition phases, and then dropped to the normal level after extinction phase, and again increased after reinstatement phase. However, there was no statistical difference in the HINT1 expression level in other three encephalic regions (PFC, CPu, and Hip). Therefore, the HINT1 protein, particularly in the NAc, plays a vital role in the METH-induced CPP. However, the precise mechanisms will require further investigation.


HINT1 Methamphetamine Conditioned place preference 



This research was supported by the National Science Foundation of China (NSFC81171262, 81771435, 81371473 to Yong-hui Dang), and the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2016JM8078 to Yong-hui Dang).

Compliance with Ethical Standards

The experimental protocol was approved by the Institutional Animal Care Committee of Xi’an Jiaotong University.

Conflict of Interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Ju-ping Li
    • 1
    • 2
  • Peng Liu
    • 1
  • Gang Lei
    • 1
  • Zheng Chu
    • 1
  • Fei Liu
    • 1
    • 3
  • Chao-feng Shi
    • 4
  • Yong-hui Dang
    • 1
    • 5
    • 6
    Email author
  • Teng Chen
    • 1
    • 5
    • 6
    Email author
  1. 1.College of Medicine and ForensicsXi’an Jiaotong University Health Science CenterXi’anPeople’s Republic of China
  2. 2.Institute of lawNorthwest University of Political Science and LawXi’anPeople’s Republic of China
  3. 3.Affiliated Stomatology Hospital of Xi’an Jiaotong University Health Science CenterXi’anPeople’s Republic of China
  4. 4.Institute of Material Evidence IdentificationKaifeng Municipal Public Security BureauKaifengPeople’s Republic of China
  5. 5.Key Laboratory of the Health Ministry for Forensic MedicineXi’an Jiaotong UniversityXi’anPeople’s Republic of China
  6. 6.Key Laboratory of Forensic Medicine of Shaanxi ProvinceXi’an Jiaotong University Health Science CenterXi’anPeople’s Republic of China

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