Neurotoxicity Research

, Volume 31, Issue 2, pp 245–258

Cytotoxic Effects of Environmental Toxins on Human Glial Cells

  • Fiona D‘Mello
  • Nady Braidy
  • Helder Marçal
  • Gilles Guillemin
  • Fanny Rossi
  • Mirielle Chinian
  • Dominique Laurent
  • Charles Teo
  • Brett A. Neilan
ORIGINAL ARTICLE

Abstract

Toxins produced by cyanobacteria and dinoflagellates have increasingly become a public health concern due to their degenerative effects on mammalian tissue and cells. In particular, emerging evidence has called attention to the neurodegenerative effects of the cyanobacterial toxin β-N-methylamino-L-alanine (BMAA). Other toxins such as the neurotoxins saxitoxin and ciguatoxin, as well as the hepatotoxic microcystin, have been previously shown to have a range of effects upon the nervous system. However, the capacity of these toxins to cause neurodegeneration in human cells has not, to our knowledge, been previously investigated. This study aimed to examine the cytotoxic effects of BMAA, microcystin-LR (MC-LR), saxitoxin (STX) and ciguatoxin (CTX-1B) on primary adult human astrocytes. We also demonstrated that α-lipoate attenuated MC-LR toxicity in primary astrocytes and characterised changes in gene expression which could potentially be caused by these toxins in primary astrocytes. Herein, we are the first to show that all of these toxins are capable of causing physiological changes consistent with neurodegeneration in glial cells, via oxidative stress and excitotoxicity, leading to a reduction in cell proliferation culminating in cell death. In addition, MC-LR toxicity was reduced significantly in astrocytes-treated α-lipoic acid. While there were no significant changes in gene expression, many of the probes that were altered were associated with neurodegenerative disease pathogenesis. Overall, this is important in advancing our current understanding of the mechanism of toxicity of MC-LR on human brain function in vitro, particularly in the context of neurodegeneration.

Keywords

Cyanobacteria Neurodegeneration Microcystin Glial cells Neuroinflammation Oxidative stress 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Fiona D‘Mello
    • 1
  • Nady Braidy
    • 2
  • Helder Marçal
    • 1
  • Gilles Guillemin
    • 3
  • Fanny Rossi
    • 4
    • 5
    • 6
    • 7
  • Mirielle Chinian
    • 8
  • Dominique Laurent
    • 4
    • 5
  • Charles Teo
    • 9
  • Brett A. Neilan
    • 1
  1. 1.School of Biotechnology and Biomolecular SciencesThe University of New South WalesSydneyAustralia
  2. 2.Centre for Healthy Brain Ageing, School of Psychiatry, Faculty of MedicineThe University of New South WalesSydneyAustralia
  3. 3.Faculty of Medicine and Health Science, Neuroinflammation groupMacquarie UniversitySydneyAustralia
  4. 4.Université de Toulouse, UPS, UMR-152 (Pharma-Dev)ToulouseFrance
  5. 5.Institut de Recherche pour le Développement (IRD), UMR-152TahitiFrench Polynesia
  6. 6.Pacific Biotech SASTahitiFrench Polynesia
  7. 7.Ecosystèmes Insulaires Océaniens, UMR-241Université de la Polynésie françaiseTahitiFrench Polynesia
  8. 8.Ecosystèmes Insulaires Océaniens UMR-241, Institut Louis Malardé, Laboratoire de recherche sur les Microalgues ToxiquesTahitiFrench Polynesia
  9. 9.Minimally Invasive Cancer CentrePrince of Wales HospitalSydneyAustralia

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