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Neurotoxicity Research

, Volume 19, Issue 1, pp 55–62 | Cite as

Memory Impairment Induced by Sodium Fluoride is Associated with Changes in Brain Monoamine Levels

  • Marcela Pereira
  • Patrícia A. Dombrowski
  • Estela M. Losso
  • Lea R. Chioca
  • Cláudio Da Cunha
  • Roberto AndreatiniEmail author
Article

Abstract

Previous studies suggest that sodium fluoride (NaF) can impair performance in some memory tasks, such as open-field habituation and two-way active avoidance. In the present study, we evaluated the effect of NaF intake (100 ppm in drinking water for 30 days) and its short-term (15 days) withdrawal on open-field habituation and brain monoamine level. Adult male rats were allocated to three groups: tap water (NaF 1.54 ppm) for 45 days (control group); 15 days of tap water followed by NaF for 30 days; and NaF for 30 days followed by 15 days of tap water. The results showed that NaF impairs open-field habituation and increases noradrenaline (NA) and serotonin (5-HT) in the striatum, hippocampus and neocortex. Dopamine (DA) increase was restricted to the striatum. Short-term NaF withdrawal did not reverse these NaF-induced changes, and both NaF treatments led to a mild fluorosis in rat incisors. No treatment effect was seen in body weight or fluid/water consumption. These results indicate that sodium fluoride induces memory impairment that outlasts short-term NaF withdrawal (2 weeks) and may be associated with NA and 5-HT increases in discrete brain regions.

Keywords

Cognitive impairment Dopamine Memory Monoamines Open-field habituation Serotonin Sodium fluoride 

Abbreviations

DA

Dopamine

NA

Noradrenaline

NaF

Sodium fluoride

DHPG

3,4-Dihydroxyphenylglycol

DOPAC

Dihydroxyphenylacetic acid

5-HIAA

5-Hydroxyindoleacetic acid

5-HT

Serotonin

HVA

Homovanilic acid

Notes

Acknowledgements

This research was partially supported by Fundação Araucária. MP, RA and CC are recipient of CNPq fellowships; PAD and LRC are recipients of CAPES fellowships.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Marcela Pereira
    • 1
  • Patrícia A. Dombrowski
    • 1
  • Estela M. Losso
    • 2
  • Lea R. Chioca
    • 1
  • Cláudio Da Cunha
    • 1
  • Roberto Andreatini
    • 1
    Email author
  1. 1.Laboratório de Fisiologia e Farmacologia do Sistema Nervoso Central, Departamento de Farmacologia, Setor de Ciências BiológicasUniversidade Federal do Paraná, Centro PolitécnicoCuritibaBrazil
  2. 2.Health Division, PedodonticsUniversidade PositivoCuritibaBrazil

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