Neurotoxicity Research

, 16:356

Time-Course Changes of Acetylcholinesterase Activity in Blood and Some Tissues in Rats After Intoxication by Russian VX

  • Jana Zdarova Karasova
  • Jiri Bajgar
  • Daniel Jun
  • Ruzena Pavlikova
  • Kamil Kuca
Article

Abstract

The toxic effect of organophosphates is attributed to irreversible inhibition of acetylcholinesterase (AChE; EC 3.1.1.7), the enzyme that hydrolyses the neurotransmitter acetylcholine. Inhibition potency in vivo of one of the most toxic nerve agents—Russian VX (RVX; N,N-diethyl-2-[methyl-(2-methylpropoxy)phosphoryl]sulfanylethanamine) (1 × LD50 dose administered intramuscularly, i.m.) was studied in rats. AChE in blood was inhibited by 50%, 3 min after i.m. RVX. Butylcholinesterase (BChE; EC 3.1.1.8) in plasma was inhibited less rapidly and only by 10–20%, 20 min after RVX. AChE and BChE activities in diaphragm were reduced only 35% and 15% at 30 min. While AChE and BChE activities were reduced only about 20% and 15%, respectively, the decline in activity was rapid, occurring within 3 min. These findings indicate that RVX most potently inhibits ChE outside the central nervous system.

Keywords

Russian VX Acetylcholinesterase Butyrylcholinesterase Nerve agent 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jana Zdarova Karasova
    • 1
  • Jiri Bajgar
    • 1
  • Daniel Jun
    • 2
  • Ruzena Pavlikova
    • 1
  • Kamil Kuca
    • 1
    • 2
    • 3
  1. 1.Department of ToxicologyFaculty of Military Health SciencesHradec KraloveCzech Republic
  2. 2.Center of Advanced StudiesFaculty of Military Health SciencesHradec KraloveCzech Republic
  3. 3.Department of ChemistryFaculty of Sciences, J.E. Purkinje UniversityUsti nad LabemCzech Republic

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