Antiparasitic effects of Zataria multiflora essential oil nano-emulsion on larval stages of Echinococcus granulosus

  • Monire Karimi Yazdi
  • Ali Haniloo
  • Azadeh Ghaffari
  • Negin TorabiEmail author
Original Article


Various protoscolicidal agents are indicated for the prevention of recurrence of cystic echinococcosis caused by the larval stage of Echinococcus granulosus; however, most of these chemicals have adverse side effects. This study evaluated the effects of Zataria multiflora essential oil (ZEO) nano-emulsion and emulsion at the concentrations of 1, 2, 5, 10, 15, and 20 µl/ml on E. granulosus protoscoleces. Albendazole (5 mg/ml), normal saline, and nano-emulsion without ZEO served as control groups. Optimal concentrations of ZEO nano-emulsion and emulsion on the microcysts were also investigated. ZEO emulsion at a concentration of 20 µl/ml for the duration of 15 min and nano-emulsion for the duration of 10 min resulted in the death of 100% of the protoscoleces. Additionally, densely packed aggregates were formed inside the microcysts treated with ZEO nano-emulsion and emulsion at a concentration of 20 µl/ml after 40 min, but the complete destruction of laminated layers did not occur. The results indicate that ZEO nano-emulsions have a higher protoscolicidal effect than its emulsion, but these two compounds had similar effects on microcysts.


Echinococcus granulosus Protoscoleces Microcyst Nano-emulsion Emulsion Zataria multiflora In vitro 



This study is a result of an approved master’s thesis from Zanjan University of Medical Sciences (A-12-95-12; ZUMS.REC.1396.172). The authors of this article would like to sincerely thank colleagues in the Department of Parasitology and Mycology at Zanjan University of Medical Sciences for their assistance in this project.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Indian Society for Parasitology 2020

Authors and Affiliations

  1. 1.Department of Parasitology and Mycology, School of MedicineZanjan University of Medical Sciences (ZUMS)ZanjanIran
  2. 2.School of PharmacyZanjan University of Medical Sciences (ZUMS)ZanjanIran

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