The first molecular detection of a Theileria-like species (Apicomplexa: Piroplasmida) in Meriones persicus from western Iran
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The archived blood of rodents species such as Meriones, Mus, and Microtus species was investigated in order to detect any piroplasms species. In this study 18S rRNA target gene of piroplasm parasite was amplified by PCR in a Meriones persicus; so, the Locus 1 and Locus 2 of 18S rRNA were sequenced, successfully. A Theileria-like taxa was suspected in accordance with the BLAST analysis of 18S rRNA L1 and L2 with 96% and 91% sequence homology, respectively. The present study was the first report of a Theileria-like species in M. persicus from Iran.
KeywordsHaemoparasite Piroplasm PCR Sanger sequencing Phylogenetic tree Iran
The researchers would like to express their gratitude to the authorities in Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, for their contribution in providing laboratory facilities. Authors deeply thank, Dr. Ahmad Mahmoudi, Ali Mohammadi, and Hamed Hanifi, the staff of Department of Epidemiology and Biostatistics of Institute Pasteur of Iran, whose participated in sampling and identification of rodent species.
AH-C designed the study. AH-C, SZH, FK, AKR, EM, and MHK collaborated to the sample collection, laboratory assays and the manuscript writing.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
We hereby declare all ethical standards have been respected in preparation of the submitted article. This research is not involving human participants. We follow the guideline for the care and use of laboratory animals National Research Council (2010). Ethical approval of Lorestan University of Medical Sciences for the research project is: LUMS.REC.1395.104.
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