Advertisement

Development of membrane-based flow-through assay for detection of trypanosomosis in equines

  • Ritesh Kumar
  • Suresh Chandra YadavEmail author
  • Sandeep Kumar
  • Neeraj DilbaghiEmail author
Original Article

Abstract

A rapid flow-through immunoassay using protein A labeled gold nanoparticles (GNPs) for the qualitative detection of trypanosomosis in equine serum samples was developed. The flow-through device consisted of antigen-coated nitrocellulose membrane fixed on absorbent pads. The GNPs based detection probe was used as the colored marker. The assay can be employed for the detection of Trypanosoma specific antibodies at 14 days post-infection in the infected pooled serum samples of equines. The assay can be conducted in < 5 min. The results of the flow-through assay were comparable with the methods like ELISA, dot blot assay and lateral flow assay. The developed assay showed high efficiency for detection of anti-Trypanosoma antibodies in equine sera and can be used for quick screening of serum samples under field conditions.

Keywords

Trypanosomosis Trypanosoma evansi Flow-through assay Gold nanoparticles Rapid immunoassay 

Notes

Acknowledgements

This study was supported by Department of Science and Technology (DST), Govt. of India under DST-IDP Project (IDP/MED/2013/09). We are grateful to the Director, National Research Centre on Equines, Hisar for providing necessary facilities to conduct the study.

Compliance with ethical standards

Conflict of interest

All authors declare that they have no conflict of interest.

Ethical approval

The animal experimentation was carried out according to rules and regulations set forth by “Committee for the Purpose of Control and Supervision of Experiments on Animals” (CPCSEA) Animal Welfare Division, Ministry of Environment, and Government of India. The research protocol for experimentation was duly approved by the “Institute Animal Ethics Committee” of the National Research Centre on Equines, Hisar, Haryana, India.

References

  1. Amendola V, Meneghetti M (2009) Size evaluation of gold nanoparticles by UV–Vis spectroscopy. J Phys Chem C 113:4277–4285.  https://doi.org/10.1021/jp8082425 CrossRefGoogle Scholar
  2. Chen P, Gates-Hollingsworth M, Pandit S et al (2019) Paper-based Vertical Flow Immunoassay (VFI) for detection of bio-threat pathogens. Talanta 191:81–88.  https://doi.org/10.1016/j.talanta.2018.08.043 CrossRefPubMedGoogle Scholar
  3. De Saeger S, Van Peteghem C (1999) Flow-through membrane-based enzyme immunoassay for rapid detection of ochratoxin A in wheat. J Food Prot 62:65–69.  https://doi.org/10.4315/0362-028X-62.1.65 CrossRefPubMedGoogle Scholar
  4. Desquesnes M, Dargantes A, Lai DH et al (2013) Trypanosoma evansi and surra: a review and perspectives on transmission, epidemiology and control, impact, and zoonotic aspects. Biomed Res Int 2013:321237.  https://doi.org/10.1155/2013/321237 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Huang S-H (2006) Gold nanoparticle-based immunochromatographic test for identification of Staphylococcus aureus from clinical specimens. Clin Chim Acta 373:139–143.  https://doi.org/10.1016/j.cca.2006.05.026 CrossRefPubMedGoogle Scholar
  6. Jans H, Liu X, Austin L et al (2009) Dynamic light scattering as a powerful tool for gold nanoparticle bioconjugation and biomolecular binding studies. Anal Chem 81:9425–9432.  https://doi.org/10.1021/ac901822w CrossRefPubMedGoogle Scholar
  7. Joshi PP, Shegokar VR, Powar RM et al (2005) Human trypanosomiasis caused by Trypanosoma evansi in India: the first case report. Am J Trop Med Hyg 73(3):491–495.  https://doi.org/10.1016/j.trstmh.2005.11.003 CrossRefPubMedGoogle Scholar
  8. Kumar A, Boruah BM, Liang X-J (2011) Gold nanoparticles: promising nanomaterials for the diagnosis of cancer and HIV/AIDS. J Nanomater 2011:202187.  https://doi.org/10.1155/2011/202187 CrossRefGoogle Scholar
  9. Kumar J, Chaudhury A, Yadav SC (2016) Comparative evaluation of recombinant HSP70 (N & C-terminal) fragments in the detection of equine trypanosomosis. Vet Parasitol 223:77–87.  https://doi.org/10.1016/j.vetpar.2016.04.015 CrossRefPubMedGoogle Scholar
  10. Kumar R, Dilbaghi N, Kumar S et al (2018) Development of lateral flow assay for point-of-care diagnosis of trypanosomosis in equines. J Equine Vet Sci 70:1–6.  https://doi.org/10.1016/j.jevs.2018.07.007 CrossRefGoogle Scholar
  11. Li ZP, Wang YC, Liu CH, Li YK (2005) Development of chemiluminescence detection of gold nanoparticles in biological conjugates for immunoassay. Anal Chim Acta 551:85–91.  https://doi.org/10.1016/j.aca.2005.07.014 CrossRefGoogle Scholar
  12. Li Y, Schluesener HJ, Xu S (2010) Gold nanoparticle-based biosensors. Gold Bull 43:29–41.  https://doi.org/10.1007/BF03214964 CrossRefGoogle Scholar
  13. Paepens C, De Saeger S, Sibanda L et al (2004) A flow-through enzyme immunoassay for the screening of fumonisins in maize. Anal Chim Acta 523:229–235.  https://doi.org/10.1016/j.aca.2004.07.044 CrossRefGoogle Scholar
  14. Peng Y, Wu J, Wang J et al (2012) Study and evaluation of Wondfo rapid diagnostic kit based on nano-gold immunochromatography assay for diagnosis of Plasmodium falciparum. Parasitol Res 110:1421–1425.  https://doi.org/10.1007/s00436-011-2643-6 CrossRefPubMedGoogle Scholar
  15. Pham VD, Hoang H, Phan TH et al (2012) Production of antibody labeled gold nanoparticles for influenza virus H5N1 diagnosis kit development. Adv Nat Sci Nanosci Nanotechnol 3:045017.  https://doi.org/10.1088/2043-6262/3/4/045017 CrossRefGoogle Scholar
  16. Preechakasedkit P, Pinwattana K, Dungchai W et al (2012) Development of a one-step immunochromatographic strip test using gold nanoparticles for the rapid detection of Salmonella typhi in human serum. Biosens Bioelectron 31:562–566.  https://doi.org/10.1016/j.bios.2011.10.031 CrossRefPubMedGoogle Scholar
  17. Roy N, Nageshan RK, Pallavi R et al (2010) Proteomics of Trypanosoma evansi infection in rodents. PLoS ONE 5(3):e9796.  https://doi.org/10.1371/journal.pone.0009796 CrossRefPubMedPubMedCentralGoogle Scholar
  18. Schneider E, Usleber E, Märtlbauer E (1995) Rapid detection of fumonisin B1 in corn-based food by competitive direct dipstick enzyme immunoassay/enzyme-linked immunofiltration assay with integrated negative control reaction. J Agric Food Chem 43:2548–2552.  https://doi.org/10.1021/jf00057a042 CrossRefGoogle Scholar
  19. Sibanda L, De Saeger S, Van Peteghem C et al (2000) Detection of T-2 toxin in different cereals by flow-through enzyme immunoassay with a simultaneous internal reference. J Agric Food Chem 48:5864–5867.  https://doi.org/10.1021/jf000337k CrossRefPubMedGoogle Scholar
  20. Silva RAMS, Arosemena NAE, Herrera HM et al (1995) Outbreak of trypanosomosis due to Trypanosoma evansi in horses of Pantanal Mato-grossense, Brazil. Vet Parasitol 60:167–171.  https://doi.org/10.1016/0304-4017(94)00757-4 CrossRefPubMedGoogle Scholar
  21. Sreedevi C, Hafeez M, Subramanyam KV et al (2011) Development and evaluation of flow through assay for detection of antibodies against porcine cysticercosis. Trop Biomed 28(1):160–170PubMedGoogle Scholar
  22. Yadav SC, Kumar R, Kumar V et al (2013) Identification of immuno-dominant antigens of Trypanosoma evansi for detection of chronic trypanosomosis using experimentally infected equines. Res Vet Sci 95:522–528.  https://doi.org/10.1016/j.rvsc.2013.04.030 CrossRefPubMedGoogle Scholar
  23. Yadav SC, Kumar R, Kumar J et al (2017) Antigenic characterization of 52–55 kDa protein isolated from Trypanosoma evansi and its application in detection of equine trypanosomosis. Res Vet Sci 114:455–460.  https://doi.org/10.1016/j.rvsc.2017.07.034 CrossRefPubMedGoogle Scholar

Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  1. 1.Department of Bio and Nano TechnologyGuru Jambheshwar University of Science and TechnologyHisarIndia
  2. 2.National Research Centre on EquinesHisarIndia

Personalised recommendations