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Journal of Parasitic Diseases

, Volume 43, Issue 4, pp 672–678 | Cite as

Molecular characterization of β-tubulin gene associated with benzimidazole resistance in larvae of field isolates of Parascaris (Nematoda: Ascarididae)

  • Seyed Hossein Malekpour
  • Ehsan RakhshandehrooEmail author
  • Azadeh Yektaseresht
Original Article
  • 9 Downloads

Abstract

Since the past 2 decades, an increasing number of resistance to the Benzimidazoles (BZs) have been reported in nematode parasites of livestock. More recently, detection of single-nucleotide polymorphisms (SNPs) at codons of 167, 198 or 200 of the β-tubulin gene has been attributed to the occurrence of resistance. In the present study, we investigated the presence of those SNPs in the β-tubulin isotype-1 gene in different isolates of Parascaris in horse. Also, the mitochondrial (mt) and ribosomal genes were sequenced for species confirmation of the isolates. The analysis of sequences inferred from COII gene confirmed that those isolates were P. equorum. The distance between mt genes obtained here and several ascarid species in equids and other hosts suggests the need for the combination of more genetic data with morphologic and other diagnostic measures. The analysis on β-tubulin isotype-1 gene revealed no resistance-related SNPs or substitutions at the expected codon positions and selection pressure with BZs has not occurred for Parascaris worms. Although the molecular data showed the susceptibility of Parascaris isolates against BZs, other mechanisms of resistance should be also investigated to confirm the validity of molecular results.

Keywords

Parascaris equorum Cytochrome oxidase 18s rRNA gene β-Tubulin gene Phylogeny 

Notes

Acknowledgements

This study was granted by Shiraz University, Shiraz, Iran (Grant No: SHM-95).

Compliance with ethical standards

Conflict of interest

Authors declared that there is no any conflict of interest.

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Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  1. 1.Department of Pathobiology, School of Veterinary MedicineShiraz UniversityShirazIran

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