Journal of Parasitic Diseases

, Volume 43, Issue 4, pp 651–657 | Cite as

Contamination of food-borne parasites from green-leafy vegetables sold in public markets of San Jose City, Nueva Ecija, Philippines

  • Khryst Celine C. VizonEmail author
  • Zosimo G. BattadII
  • Diane Shiela C. Castillo
Original Article


Vegetables contribute a significant portion of the typical Filipino diet. Hence, it is a concern that based on previous studies, vegetables could potentially act as vectors for parasitic transmission. This study aimed to further investigate these occurrences by assessing the food-borne parasitic contamination of vegetables sold in the public markets of San Jose City, Nueva Ecija. A total of 288 vegetable samples (cabbage, lettuce and Chinese cabbage) were collected from March to August 2018 in the wet market and supermarkets of San Jose City, Nueva Ecija. Results showed that 116 out of 288 (40.28%) vegetable samples were positive of food-borne parasites such as Ascaris sp., Hymenolepis diminuta, Isospora sp., Hookworm/Strongylid sp./free-living nematode larvae, Strongylid sp. egg, Taenia sp., and Trichuris sp. Isospora sp. has the highest contamination rate (16.67%) and mean density (13 eggs/g) from the sampled vegetables. Possible sources of these identified parasites are contaminated soil and water harbored by the sampled vegetables during cultivation, transport and handling/preparation by vendors. Further studies are needed to establish a more reliable information that could lead to public health improvement and food safety awareness for the community.


Parasitic contamination Vegetables Unwashed versus treated Food-borne parasites 



We would like to thank the Department of Biological Sciences, Central Luzon State University for the equipment and facilities provided for this study.

Authors contribution

Conceived and designed the experiments: KCCV, ZGB, DSCC; Performed the experiment, Data analysis: KCCV; All authors participated in writing the final paper.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Abe E, Ajah L, Ayuba S, Mogaji H, Ekpo U (2016) Geohelminths contamination fruits and vegetables sold in Lafia Markets. Annu Res Rev Biol 11(2):1–8CrossRefGoogle Scholar
  2. Al-Megrin WAI (2010) Prevalence of intestinal parasites in leafy vegetables in Riyadh, Saudi Arabia. Int J Trop Med 5(2):20–23CrossRefGoogle Scholar
  3. Choi DW, Lee S (1972) Incidence of parasites found on vegetables collected from markets and vegetable gardens in Taegu Area. Korean J Parasitol 10(1):44–51CrossRefGoogle Scholar
  4. Duedu KO, Yarnie EA, Tetteh-Quarco PB, Attah SK, Donkor ES, Ayeh-Kumi PF (2014) A comparative survey of the prevalence of human parasites found in fresh vegetables sold in supermarkets and open-aired markets in Accra, Ghana. BMC Res Notes 7(1):1–6CrossRefGoogle Scholar
  5. Ebrahimzadeh A, Jamshidi A, Mohammadi S (2013) The parasitic contamination of raw vegetables consumed in Zahedan. Iran. Health Scope 1(4):202–206Google Scholar
  6. Fallah AA, Makhtumi Y, Pirali-Kheirabadi K (2015) Seasonal study of parasitic contamination in fresh salad vegetables marketed in Shahrekord, Iran. Food Control 1:20. CrossRefGoogle Scholar
  7. Gupta N, Khan DK, Santra SC (2009) Prevalence of intestinal helminth eggs on vegetables grown in wastewater-irrigated areas of Titagarh, West Bengal, India. Food Control 20:942–945CrossRefGoogle Scholar
  8. Hajjami K, Ennaji MM, Amdiouni H, Fouad S, Cohen N (2013) Parasitic contamination fresh vegetable consumed in Casablanca City (Morocco) and risk from consumer. Int J Sci Technol 2(7):543–549Google Scholar
  9. Hall EJ (2013) Chapter 57: small intestine. In: Washabau RJ, Day MJ (eds) Canine and feline gastroeneterology. Elsevier-Saunders, North York, pp 651–728Google Scholar
  10. Kłapeć T, Borecka A (2012) Contamination of vegetables, fruits and soil with geohelminths eggs on organic farms in Poland. Ann Agric Environ Med 19(3):421–425PubMedGoogle Scholar
  11. Kozan E, Sevimi FK, Kose M, Eserm M, Cicek H (2007) Examination of helminth contaminated wastewaters used for agricultural purposes in Afyonkarahisar. Turkiye Parazitol Derg 31:197–200PubMedGoogle Scholar
  12. Maipanich W, Chaisiri K, Yoonuan T, Sato M, Sato MO, Pongvongsa T et al (2011) Zoonotic helminth contamination of the environment in rural villages of Southern Lao PDR. J Trop Med Parasitol 34(2):54–61Google Scholar
  13. Ogbolu D, Alli O, Ogunleye V, Olusoga-Ogbolu F, Olaosun I (2009) The presence of intestinal parasites in selected vegetables from open markets in South Western Nigeria. Afr J Med Med Sci 38(4):319–324PubMedGoogle Scholar
  14. Ordoñez K, Paller VG, Goh XT, Lim Y (2018) Parasite contamination of vegetables from selected organic and conventional farms in Laguna and Benguet Province, Philippines. Trop Agric Sci 41(4):1741–1746Google Scholar
  15. Parasites (2018) Accessed 1 May 2019
  16. Su GLS, Mariano CMR, Matti NSA, Ramos GB (2012) Assessing parasitic infestation of vegetables in selected markets in Metro Manila, Philippines. Asian Pac J Trop Dis 2(1):51–54. CrossRefGoogle Scholar
  17. Suh KN, Kozarsky P, Keystone JS (2015) Cyclospora cayetanensis, Cytoisospora (Isospora) belli, Sarcocystis species, Balantidium coli, and Blastocystis species. In: Bennett JE, Dolin R, Blaser MJ (eds) Mandell Douglas, and Bennett’s principles and practice of infectious diseases, 8th edn. Elsevier-Saunders, North York, pp 3184–3191Google Scholar

Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  • Khryst Celine C. Vizon
    • 1
    Email author
  • Zosimo G. BattadII
    • 2
  • Diane Shiela C. Castillo
    • 1
  1. 1.Department of Environmental Science, College of Arts and SciencesCentral Luzon State UniversityScience City of MuñozPhilippines
  2. 2.Department of Biological Sciences, College of Arts and SciencesCentral Luzon State UniversityScience City of MuñozPhilippines

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