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Phylogenetic analysis of goat warble fly (Przhevalskiana silenus) based on mitochondrial COI gene

  • Ehsan RakhshandehrooEmail author
  • Seyed Mostafa Razavi
  • Razieh Farzaneh
  • Atefeh Esmailnejad
  • Mohammad Asadpour
  • Shahab Shams
Original Article
  • 8 Downloads

Abstract

The larvae of the genus Przhevalskiana (Diptera: Oestridae) are the causative agents of subcutaneous myiasis in goats. Several species have been grouped under this genus based on the morphology of different larval stages, albeit with a lot of uncertainties. Thus, application of genetic tools seems to be helpful for taxonomy. During this study, the cytochrome oxidase I (COI) gene was targeted for the characterization of larval stages of goat warble fly. Fragments of 606 bp were amplified for all the specimens. Based on the COI gene analysis, all the recovered specimens were identified as larvae of Przhevalskiana silenus. Molecular data on the genus is relatively rare but present isolates revealed about 87–89% identity with previous isolates of P. silenus. According to the phylogenetic data, the present isolates branched (as a sister group) with a number of Hypoderma spp. including H. bovis, H. diana, H. lineatum and H. sinense. The present findings confirmed that the COI gene could be a suitable marker for genetic characterization and identification of larvae up to the species level.

Keywords

Przhevalskiana silenus Goat warble fly Cytochrome oxidase I (COI) gene Phylogeny 

Notes

Acknowledgements

The authors thank Mr. Mootabi Alavi for his kind help.

Author’s contribution

ER and SMR designed and supervised the experiment. RF carried out the research technically; AE and MA had roles in analyzing data and writing the manuscript. SS participated in collecting the samples and conducting the study.

Funding information

This work was financially supported by a research grant from Shiraz University, Shiraz, Iran (Grant No. 96-GR-VT-1).

Compliance with ethical standards

Conflict of interest

All authors declares that they have no conflict of interest.

References

  1. Le Riche P, Jorgensen R, Campbell J, Efstathiou G (1973) Notes on the goat warble fly, Przhevalskiana aegagri Brauer, 1863, in Cyprus. J Nat Hist 7:615622CrossRefGoogle Scholar
  2. Lunt DH, Zhang DX, Szymura JM, Hewltt OM (1996) The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 5:153–165CrossRefPubMedGoogle Scholar
  3. Madel G (1969) The goat warble-fly Przhevalskiana silenus in Afghanistan. Angew Parasitol 10:204–210Google Scholar
  4. Oryan A, Bahrami S (2012) Pathology of natural Przhevalskiana silenus infestation in goats. Trop Biomed 29:524–531PubMedGoogle Scholar
  5. Otranto D, Puccini V (2000) Further evidence on the internal life cycle of Przhevalskiana silenus (Diptera, Oestridae). Vet Parasitol 88:321–328CrossRefPubMedGoogle Scholar
  6. Otranto D, Traversa D (2004) Molecular evidence indicating that Przhevalskiana silenus, P. aegagri and P. crossii (Diptera, Oestridae) are one species. Acta Parasitol 49:173–176Google Scholar
  7. Otranto D, Tarsitano E, Giangaspero A, Puccini V (2000) Differentiation by polymerase chain reaction-restriction fragment length polymorphism of some Oestridae larvae causing myiasis. Vet Parasitol 90:305–313CrossRefPubMedGoogle Scholar
  8. Otranto D, Traversa D, Guida B, Tarsitano E, Fiorente P, Stevens JR (2003) Molecular characterization of the mitochondrial cytochrome oxidase I (COI) gene of Oestridae larvae causing obligate myiasis. Med Vet Entomol 17:307–315CrossRefPubMedGoogle Scholar
  9. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599CrossRefPubMedGoogle Scholar
  10. Tamura K, Stecher G, Peterson D, Peterson Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729CrossRefPubMedGoogle Scholar
  11. Xiong B, Kochner TD (1991) Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae). Genome 34:306–311CrossRefPubMedGoogle Scholar
  12. Zhang DX, Hewitt GM (1997) Assessment of the universality and utility of a set of conserved mitochondrial COI primers in insects. Insect Mol Biol 6:143–150CrossRefPubMedGoogle Scholar
  13. Zhang DX, Szymura JM, Hewitt GM (1995) Evolution and structural conservation of the control region of insect mitochondrial DNA. J Mol Evol 40:382–391CrossRefPubMedGoogle Scholar
  14. Zumpt F (1965) Myiasis in man and animals of the old world. Butterworths, London, pp 205–214Google Scholar

Copyright information

© Indian Society for Parasitology 2019

Authors and Affiliations

  1. 1.Department of Pathobiology, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Department of Clinical Studies, School of Veterinary MedicineShiraz UniversityShirazIran

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