Therapeutic potential effect of bone marrow-derived mesenchymal stem cells on chronic liver disease in murine Schistosomiasis Mansoni
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Some reports have shown that mesenchymal stem cells (MSCs) therapy could ameliorate chemically-induced hepatic fibrosis. This research assesses the therapeutic action of bone marrow mesenchymal stem cells (BM-MSCs) on chronic diseased liver in Schistosoma mansoni infected mice. All infected female mice divided into three groups, one group (15 mice) treated with oral praziquantel (PZQ), second group (15 mice) received intravenous injection of BM-MSCs and third group (15 mice) treated with both MSCs + PZQ. Two control groups (15 mice each) subdivided into one infected and second healthy one. BM-MSCs were obtained from bones of both femur and tibia of male mice (30 mice), then cultured and characterized morphologically by detection of CD105 by flow cytometer. Liver tissues for all groups were examined histopathologically. Measuring of the collagen 1 gene expression was done by real-time PCR and immunohistochemical study to detect stem cells differentiation for detection of MSCs engraftments in liver tissue. MSCs treatment caused marked improvement and regression of fibrosis, and prevents deposition of collagen and reduced the expression of collagen 1 gene in infected mice on their liver tissues, especially when used with PZQ in mice treatment. It can be concluded that, MSCs is a good therapeutic method for liver fibrosis caused by S. mansoni infection.
KeywordsMSCs Schistosoma mansoni Praziquantel Collagen 1 gene OV-6
We thank Dr.Soheir Sayed Mahmoud, Professor of Parasitology in Theodor Bilharze Research Institute, for her valuable advices during animal experimentation. Further we thank Dr.Olfat Aly Hammam, Professor of Pathology in Theodor Bilharze Research Institute, Egypt for her active participation in histopathological and immunohistochemical parts of this work.
MH Abdel-Hady and SM Nada shared in the study design and research topics. SH Abd-Allah initiated the research idea and shared in performing the laboratory work of obtaining BM-MSCs. MS Badawey shared in the study design, in performing the laboratory work, and reviewing the manuscript. AS Metwally, GM Fathy, SA Abdel-Rahman and Amira A. Saleh shared in the laboratory work, interpretation of the results, collecting references, wrote and reviewed the manuscript. Ayman A. Saleh and MAbu El-Magd shared in real-time PCR study to detect stem cells differentiation. A fawzy, shared in performing the laboratory work of obtaining BM-MSCs, cultured and characterization.
Compliance with ethical standard
Conflict of interest
Authors declare that there is no any conflict of interests.
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