Thermotolerant Acanthamoeba spp. isolated from recreational water in Gorgan City, north of Iran
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Acanthamoeba as free-living parasites are scattered ubiquitously, throughout the world. This study was aimed to evaluate the presence of Acanthamoeba spp. genotypes in the recreational water sources in Gorgan County, the capital of Golestan Province using both morphological and molecular approaches. Thirty water samples were collected from different recreational waters in Gorgan, the capital of Golestan Province, northern Iran during 2015–2016. Samples were filtered and followed by culture in non-nutrient agar. Acanthamoeba were identified both by morphological and molecular analysis. The pathogenical potential of positive cloned samples were also determined using tolerance test. Twenty-six percent of recreational water were identified as Acanthamoeba spp. based on the morphological analysis and from these positive samples, five samples were successfully sequenced after molecular studies. Phylogenetic analysis showed the clustering of four samples in T4 genotype group and only one sample as T15 genotype. Thermotolerance test revealed that all cloned samples were highly positive. Since the attractiveness of recreational places for people is increasing, the potential risk of this water should be monitored routinely in each region. More studies are needed to better evaluate the risk of this ubiquitous parasite for the human.
KeywordsAcanthamoeba Genotyping techniques Morphological Molecular Iran
The authors would like to express their gratitude to Mrs. Shahrbanoo Naderi for her useful collaboration.
All authors contributed to the study design. RS was leader of the research. SM, EM, AT, MHM and MAY carried out experimental tests and prepared the Manuscript. All authors read and approved the final version of the manuscript.
Funding was provided by Golestan University of Medical Sciences.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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