Preparation and characterization of excretory and secretory antigen of Cotylophoron cotylophorum and Gastrothylax crumenifer
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Excretory and secretory antigen of adult Cotylophoron cotylophorum and Gastrothylax crumenifer was prepared and characterized by SDS-PAGE. Adult paramphistomes were collected in PBS from rumen of sheep slaughtered at Perambur and Villivakkam slaughter houses, Chennai, Tamil Nadu. Excretory/Secretory (E/S) antigens of G. crumenifer and C. cotylophorum were prepared by incubating in PBS at 28.3 °C overnight in a shaking incubator and centrifuged. The culture supernatant was used as antigen. The protein concentration of E/S antigens of C. cotylophorum and G. crumenifer ranged from 0.4 to 7.1 mg/ml and 3.3 to 5.9 mg/ml, respectively. SDS-PAGE analysis of E/S antigen of C. cotylophorum revealed two polypeptide bands from the range of 32 and 40 kDa. Whereas, SDS-PAGE analysis of E/S antigen of G. crumenifer revealed only one polypeptide band of 35 kDa. Excretory/secretory antigen may be used for serodiagnosis of paramphistomosis infection in sheep.
KeywordsParamphistomes Cotylophoron cotylophorum Gastrothylax crumenifer E/S antigen sheep Tamil Nadu
GA Collection, preparation and characterization of ES antigen of paramphistomes; CS collection of literature and preparation of manuscript; KN Assistance in literature collection, typing and preparation of manuscript; SG Assistance in preparation of manuscript.
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
Since, the study was carried out by collection of paramphistomes from the slaughtered sheep and there is no involvement of experimental animals in this study.
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