Journal of Parasitic Diseases

, Volume 41, Issue 2, pp 467–472 | Cite as

In vitro ovicidal activity of Peganum harmala seeds extract on the eggs of Fasciola hepatica

  • Mohammad MoazeniEmail author
  • Zahra Sadat Saadaty Ardakani
  • Mohammad Jamal Saharkhiz
  • Jafar Jalaei
  • Ali Asghar Khademolhoseini
  • Shahab Shams Esfand Abad
  • Amir Mootabi Alavi
Original Article


Peganum harmala seeds extract has been previously reported to have antimicrobial and other medicinal properties. The aim of this study was to evaluate the ovicidal activity of the methanolic extract of P. harmala seeds against the eggs of F. hepatica. The phenolic compounds of the methanolic extract of P. harmala seeds were identified by HPLC analysis. Catechin, rutin, p-Coumaric acid, chloregenic acid and hesperetin were found to be the major phenolic compounds. F. hepatica eggs were collected from the gall bladder of naturally infected sheep. The eggs were exposed to two concentrations of P. harmala seeds extract (1 and 3 mg/mL) for 24 and 48 h. To investigate the effect of the P. harmala seeds extract on the miracidial formation, the treated eggs were incubated at 28 °C for 14 days. The results indicated that F. hepatica eggs were susceptible to the methanolic extract of P. harmala seeds. Following 24 h exposure of the eggs to P. harmala seeds extract with concentrations of 1 and 3 mg/mL, the miracidial formation reduced to 5 and 2.2 % respectively (compared with 60 % for the control group). Following 48 h of exposure of the eggs to P. harmala seeds extract with 1 mg/mL concentration, the miracidial formation reduced to 0.5 %. In this exposure time, no miracidial formation was observed in the eggs exposed to P. harmala seeds extract with concentration of 3 mg/mL. Therefore, the results of this study indicated that P. harmala seeds extract has high ovicidal activity against the eggs of F. hepatica. Accordingly, this extract may have the potential flukicidal activity against the immature and mature F. hepatica.


Fasciola hepatica Miracidial formation Ovicidal Methanolic extract Peganum harmala Phenolic compounds 


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Copyright information

© Indian Society for Parasitology 2016

Authors and Affiliations

  • Mohammad Moazeni
    • 1
    Email author
  • Zahra Sadat Saadaty Ardakani
    • 1
  • Mohammad Jamal Saharkhiz
    • 2
  • Jafar Jalaei
    • 3
  • Ali Asghar Khademolhoseini
    • 1
  • Shahab Shams Esfand Abad
    • 1
  • Amir Mootabi Alavi
    • 1
  1. 1.Department of Pathobiology, School of Veterinary MedicineShiraz UniversityShirazIran
  2. 2.Department of Horticulture, College of AgricultureShiraz UniversityShirazIran
  3. 3.Department of Pharmacology and Toxicology, School of Veterinary MedicineShiraz UniversityShirazIran

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