Ultrastructural studies on Setaria digitata by scaning electron microscopy
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Abstract
Ultrastructural details of adult male and female worms of Setaria species collected from the peritoneal cavities of bovines slaughtered in and around Hyderabad, Telangana, India were studied using scanning electron microscopy. The worms exhibited peribuccal crown with round mouth opening encircled by a pair of lateral appendages and slightly notched dorso and ventro projections at the anterior end where as bosses and fine cuticular striations were seen all over the body. Posterior end of female worm ended in a round knob with a pair of lateral appendages whereas three pairs of precloacal, a pair of adcloacal, three pairs of postcloacal papillae, a central papillae just in front of the cloaca, a single tongue shaped spicule emerging from the cloaca, and a phasmid in front of a pair of lateral appendages were seen at the posterior end of male worm. The ultrastructural details confirmed the worms as Seteria digitata.
Keywords
Setaria digitata SEM BovineNotes
Acknowledgements
First author is thankful to Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India for the financial support in the form of P.G. stipend and for providing facilities at RUSKA Laboratory College of Veterinary Science, SVVU, Rajendranagar, Hyderabad, Telangana state, India to carryout the research work.
References
- Almeida AJ, Deobhankar KP, Bhopale MK, Zaman V, Renapurkar DM (1991) Scanning electron microscopy of Setaria cervi adult male worms. Int J Parasitol 21:119–121Google Scholar
- Chabaud AG, Bain O (1994) The evolutionary expansion of the Spirurida. Int J Parasitol 24:1179–1201CrossRefPubMedGoogle Scholar
- Dhas KPL, Decruse SW, Raj RK (1993) Synthesis and release of proteins homologous to excretory-secretory antigens during embryogenesis of Setaria digitata. J Biosci 18:311–318CrossRefGoogle Scholar
- Fujita J, Imai S, Ishii T, Nunoya T, Takahashi K, Tomita T, Oikawa R (1985) Heterotropic parasitism of Setaria digitata (Linstow, 1906) in the heart of a cattle. Jpn J Vet Sci 47(6):999–1002 CrossRefGoogle Scholar
- Innes JRM, Shoho C (1953) Cerebrospinal nematodiasis: focal encephalomyelomalacia of animals caused by nematodes (Setaria digitata); a disease which may occur in man. Arch neurol psyuchiat 70:325–349Google Scholar
- Jayasinghe DE, Wijesundera WSS (2003) Differentiation of Setaria digitata and Setaria labiatopapillosa using molecular markers. Vet J 165:136–142CrossRefPubMedGoogle Scholar
- Kim NS, Kim HC, Sim C, Ji JR, Kim NS, Park BK (2010) Congenital infection with Setaria digitata and Setaria marshalli in the thoracic cavity of a Korean calf: a case report. Vet Med Czech 55:275–280Google Scholar
- Nakano H, Tozuka M, Ikadai H, Goto R, Kudo N, Katayama Y, Muranaka M, Anzai T, Oyamada T (2006) Morphological survey of bovine Setaria in the abdominal cavities of cattle in aomori and kumamoto prefectures, Japan. J Vet Med Sci 69:413–415Google Scholar
- Rhee JK, Choi EY, Park BK, Jang BG (1994) Application of scanning electron microscopy in assessing the prevalence of some Setaria species in Korean cattle. Korean J Parasitol 32:1–6CrossRefPubMedGoogle Scholar
- Shoho C (1958) Studies on Cerebrospinal nematodiasis in Ceylon. Ceylon Vet J 6:10–15Google Scholar
- Shoho C, Uni S (1977) Scanning electron microscopy (SEM) of some Setaria species (Filaroidea, Nematoda). Z Parasitenkd 53:93–104Google Scholar
- Sigraskar SU, Chouduri PC, Singhari NA (1999) Bubaline microfilariasis : i epizootiological studies. Buffalo bull 18:64–66Google Scholar
- Soulsby EJL (1982) Helminths, arthropods and protozoa of domestic animals. Bailliere Tindall, London, pp 317–318Google Scholar
- Subhachalat P, Shirasaka S, Nakajima H, Adachi Y (1999) Setaria digitata in cattle of Thailand identified by sodium dodecyle sulphate polyacrylamide gel electrophoresis. J Vet Med Sci 61:443–445Google Scholar
- Whitlock JH (1960) Diagnosis of parasitisms 1st ed Lea & Febger Philadelphia, p 208Google Scholar
- Yadav AK, Tandon V (1991) Scanning electron microscopy of Setaria digitata (Nematode:Filaroidea). J Vet Parasitol 5:76–80Google Scholar
- Yatawara L, Wickramasinghe S, Nagataki M, Rajapakse RPVJ, Agatsuma T (2007) Molecular characterization and phylogenetic analysis of Setaria digitata of Sri Lanka based on CO1 and 12SrDNA genes. Vet Parasitol 148:161–165Google Scholar