Journal of Parasitic Diseases

, Volume 40, Issue 3, pp 1009–1013 | Cite as

Histopathological and bacterial study of skin and gill of grass carp, Ceteopharyngodon idella, (Valenciennes 1844) exposed to copper sulfate and potassium permanganate

  • Fatemeh Jooyandeh
  • Ali Sadeghpour
  • Hossein KharaEmail author
  • Zabihollah Pajand
Original Article


The gill histology and bacterial load of skin of the grass carp juveniles were investigated in relation to various concentrations of copper sulfate and potassium permanganate. For this purpose, the sublethal doses were determined after a pre-test and then the experiment was done in five treatments (for copper sulfate: 1, 1.94, 3.71, 7.07 and 15 mg/l and for potassium permanganate: 0.25, 0.52, 1.91, 2.27 and 5 mg/l) with three replicates inside the glass aquaria. Also, one group without disinfecting product was considered as control for each experiment. The microbial and histopathological investigations were done after 96 h exposure. According to results, the lowest bacterial load (CFU/g) of skin was observed in 15 mg/l copper sulfate treatment and 0.25 mg/l potassium permanganate treatment (P < 0.05). Also, the histological investigation showed a range of histopathological alternations in gills tissue including lamellar necrosis, hyperplasia, lamellar adhesion, haemorrhage, clubbing of gill lamellae. The severity of these alternations increased with increasing of the doses of the copper sulfate and potassium permanganate. In this regard, the highest histological damages were observed in 15 mg/l copper sulfate and 5 mg/l potassium permanganate respectively. Our results showed that low dosage of potassium permanganate has best effect on reducing of bacterial load of skin with lowest adverse effects on gill tissue.


Copper sulfate Potassium permanganate Histology Gill Grass carp 


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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Fatemeh Jooyandeh
    • 1
  • Ali Sadeghpour
    • 1
  • Hossein Khara
    • 1
    Email author
  • Zabihollah Pajand
    • 2
  1. 1.Department of Fishery, College of Natural Resources, Lahijan BranchIslamic Azad UniversityLahijanIran
  2. 2.The International Research Institute of SturgeonRashtIran

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